Topic
Cyclase
About: Cyclase is a research topic. Over the lifetime, 10162 publications have been published within this topic receiving 388566 citations.
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TL;DR: The results strongly suggest that guanine nucleotide binding proteins are involved in T. cruzi adenylate cyclase activation and may be useful for studying cell differentiation mechanisms in eukaryotes.
110 citations
TL;DR: The ability of glucagon to increase hepatocyte intracellular cyclic AMP concentrations was reduced markedly by the tumour‐promoting phorbol ester TPA, and it is suggested that TPA exerts its inhibitory effect on adenylate cyclase through the action of protein kinase C.
110 citations
TL;DR: Evidence that transformed cells have an increased NADH/NAD ratio and decreased adenylate cyclase and evidence that NADH will inhibit the AMP cyclase of plasma membrane has brought up the question of a sensor of NADH in the plasma membrane.
110 citations
TL;DR: In this paper, an improved system for AC-Hly synthesis and activation in Escherichia coli was developed, and the results showed that increased expression of the cyaC gene leads to a higher proportion of activated ACHly, while its invasive and hemolytic activity was higher.
Abstract: Bordetella pertussis adenylate cyclase-hemolysin (AC-Hly), encoded by the cyaA gene, belongs to the RTX family of toxins with extensive glycine-rich repeats in the carboxy-terminal portion. AC-Hly possesses both adenylate cyclase toxic and hemolytic activities that depend on a posttranslational modification mediated by the product of the cyaC gene. An improved system for AC-Hly synthesis and activation in Escherichia coli was developed. The results show that with purified AC-Hly (i) increased expression of the cyaC gene leads to a higher proportion of activated AC-Hly, (ii) the increase in protective activity of the activated recombinant AC-Hly correlates with the increase in its invasive and hemolytic activities, and (iii) the activated recombinant AC-Hly, but not the nonactivated recombinant AC-Hly, is a protective antigen against B. pertussis infection in a murine respiratory model. This suggests that possibly an immunodominant epitope required for protective activity is linked to the CyaC-mediated modification. Surprisingly, the protective and hemolytic activities of activated recombinant AC-Hly were lower than those of AC-Hly produced by B. pertussis, while its invasive activity was higher. This indicates that the modification of AC-Hly in B. pertussis and that in E. coli may differ.
110 citations
110 citations