Topic
Cyclase
About: Cyclase is a research topic. Over the lifetime, 10162 publications have been published within this topic receiving 388566 citations.
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TL;DR: Genomic and cDNA clones for vetispiradiene synthase, a sesquiterpene cyclase found in Hyoscyamus muticus, were isolated using a combination of reverse transcription-polymerase chain reactions and conventional cloning procedures and showed sequence similarity between the tobacco and H. muticus enzymes.
185 citations
TL;DR: Removal of calcium by EDTA appear to increase PGE stimulation of adenyl cyclase without altering NaF stimulation, confirming the hypothesis that calcium plays a role in regulating the sentivity of cell membranes to hormones or the hormone-like prostaglandins.
184 citations
TL;DR: This work has utilized purified reactants and cofactors to examine the form of the stimulatory guanine nucleotide-binding regulatory component of adenylate cyclase that serves as a substrate for ADP-ribosylation by cholera toxin and investigated some of the consequences of that covalent modification.
184 citations
TL;DR: Cell membranes were prepared from turkey erythrocytes by fragmentation into small particles or larger pieces which led to a loss of responsiveness to epinephrine and could be restored by prior incubation with a sulfhydryl reagent such as mercaptoethanol.
183 citations
TL;DR: Data point to a significant phenotypic change having taken place in this clonal cell line with prolonged maintenance in culture, with the emergence of a calcitonin receptor linked to adenylate cyclase and protein kinase activation.
Abstract: The clonal cell line UMR 106, which was originally derived from a rat transplantable osteogenic sarcoma with an osteoblastic phenotype, was subcloned after the emergence of a calcitonin-responsive adenylate cyclase was noted in late passages. Detailed studies on the stimulation of adenylate cyclase and activation profile of the cyclic AMP-dependent protein kinase isoenzymes in response to parathyroid hormone (PTH) and salmon calcitonin (SCT) were conducted on two subclones (UMR 106-01 and UMR 106-06). Both subclones responded in an identical manner to PTH, which stimulated adenylate cyclase and activated both isoenzyme I and isoenzyme II of cyclic AMP-dependent protein kinase. In contrast, only UMR 106-06 cells responded to calcitonin. At 3×10−8M SCT, there was a sevenfold stimulation of adenylate cyclase, 84% activation of isoenzyme I, and 44% activation of isoenzyme II. The activation profiles of the isoenzymes to PTH and SCT in UMR 106-06 were similar. Furthermore, their response to SCT correlates with the presence of specific, saturable binding of125I-labeled SCT. Binding parameters indicate apparent Kd=0.8 nM and 6,000 receptors/cell. These data point to a significant phenotypic change having taken place in this clonal cell line with prolonged maintenance in culture, with the emergence of a calcitonin receptor linked to adenylate cyclase and protein kinase activation.
182 citations