Dalfopristin

About: Dalfopristin is a research topic. Over the lifetime, 696 publications have been published within this topic receiving 26621 citations. The topic is also known as: RP-54476 & Dalfopristina.

Antibiotic resistance and mechanisms implicated in fecal enterococci recovered from pigs, cattle and sheep in a Portuguese slaughterhouse

Abstract: A total of 194 enterococcal isolates were recovered from 198 fecal samples of pigs, cattle, and sheep obtained in a Portuguese slaughterhouse. The enterococcal species most prevalent were Enterococcus faecium and E. hirae. High percentages of resistance were detected for tetracycline in pig isolates (95.7%), sheep isolates (76.7%), and cattle isolates (49%); erythromycin resistance was higher in pig isolates than in cattle or sheep isolates. Intermediate level of resistance was obtained to quinupristin/dalfopristin in all animal isolates (15.1–23.5%). High-level resistance to aminoglycosides was detected, HLR-S and -K was higher in pig isolates (44.3 and 32.9%, respectively) compared with cattle or sheep isolates, and modest percentages of HLR-G were obtained in pig and cattle isolates (7.1 and 3.9%, respectively). The aac(6`)-aph(2”), aph(3`)-IIIa, ant(6)-Ia, cat(A), erm(B), and tet(M) genes were demonstrated in most of the gentamycin-, kanamycin-, streptomycin-, chloramphenicol-, erythromycin- and tetracycline-resistant isolates, respectively. The association between the tet(M) gene and Tn916/Tn1545-like or Tn5397-like transposons was detected in 30.8 and 11.2% of the isolates, respectively. Food animals could be a reservoir for antibiotic resistance genes, and slaughterhouse cross-contamination of animals carcasses may be a food safety risk.

Enhancing the Antibacterial Activity of Erythromycin with Titanium Dioxide Nanoparticles against MRSA.

Abstract: Background Staphylococcus aureus (S. aureus) is the most common infectious agent in the community and hospitals. Infections with S. aureus are now becoming difficult to be treated by using conventional antibiotics due to its emerging methicillin-resistant S. aureus (MRSA) strain. Objective In the present study, MRSA was isolated from clinical samples and evaluated for resistance against different antibiotics, TiO2 nanoparticles, and their combinations. Methods Clinical samples were collected from Ayub Medical Complex (AMC), Abbottabad, Pakistan, and identified by different biochemical tests and polymerase chain reactions (PCR). Kirby-Bauer disk diffusion method was performed to evaluate antimicrobial susceptibility. Minimum Inhibitory Concentration (MIC) of ampicillin, ciprofloxacin, erythromycin, and vancomycin was found out by agar dilution method while the broth dilution method was used for the MIC of TiO2 nanoparticles and their combinations with erythromycin. Results All 13/100 (13%) MRSA were successfully identified. All isolates were susceptible to quinupristin/ dalfopristin, teicoplanin, and vancomycin, while the highest resistance was seen with erythromycin, penicillin, and tetracycline. MIC showed high resistance against ampicillin (0.25-512 mg/L) and erythromycin (0.25-1024 mg/L). Conclusion The MIC value of 2 mM TiO2 nanoparticles was found to be the most effective concentration after 12 h of incubation, while the combination of erythromycin with 3 mM TiO2 nanoparticles was found to be more potent which significantly lowered down the MIC of erythromycin to 2-16 mg/L.