De Bruijn graph

About: De Bruijn graph is a research topic. Over the lifetime, 690 publications have been published within this topic receiving 24735 citations.

Velvet: Algorithms for de novo short read assembly using de Bruijn graphs

Abstract: We have developed a new set of algorithms, collectively called "Velvet," to manipulate de Bruijn graphs for genomic sequence assembly. A de Bruijn graph is a compact representation based on short words (k-mers) that is ideal for high coverage, very short read (25-50 bp) data sets. Applying Velvet to very short reads and paired-ends information only, one can produce contigs of significant length, up to 50-kb N50 length in simulations of prokaryotic data and 3-kb N50 on simulated mammalian BACs. When applied to real Solexa data sets without read pairs, Velvet generated contigs of approximately 8 kb in a prokaryote and 2 kb in a mammalian BAC, in close agreement with our simulated results without read-pair information. Velvet represents a new approach to assembly that can leverage very short reads in combination with read pairs to produce useful assemblies.

Introduction to Parallel Algorithms and Architectures: Arrays, Trees, Hypercubes

Abstract: Preface Acknowledgments Notation 1 Arrays and Trees 1.1 Elementary Sorting and Counting 1.1.1 Sorting on a Linear Array Assessing the Performance of the Algorithm Sorting N Numbers with Fewer Than N Processors 1.1.2 Sorting in the Bit Model 1.1.3 Lower Bounds 1.1.4 A Counterexample-Counting 1.1.5 Properties of the Fixed-Connection Network Model 1.2 Integer Arithmetic 1.2.1 Carry-Lookahead Addition 1.2.2 Prefix Computations-Segmented Prefix Computations 1.2.3 Carry-Save Addition 1.2.4 Multiplication and Convolution 1.2.5 Division and Newton Iteration 1.3 Matrix Algorithms 1.3.1 Elementary Matrix Products 1.3.2 Algorithms for Triangular Matrices 1.3.3 Algorithms for Tridiagonal Matrices -Odd-Even Reduction -Parallel Prefix Algorithms 1.3.4 Gaussian Elimination 1.3.5 Iterative Methods -Jacobi Relaxation -Gauss-Seidel Relaxation Finite Difference Methods -Multigrid Methods 1.4 Retiming and Systolic Conversion 1.4.1 A Motivating Example-Palindrome Recognition 1.4.2 The Systolic and Semisystolic Model of Computation 1.4.3 Retiming Semisystolic Networks 1.4.4 Conversion of a Semisystolic Network into a Systolic Network 1.4.5 The Special Case of Broadcasting 1.4.6 Retiming the Host 1.4.7 Design by Systolic Conversion-A Summary 1.5 Graph Algorithms 1.5.1 Transitive Closure 1.5.2 Connected Components 1.5.3 Shortest Paths 1.5.4 Breadth-First Spanning Trees 1.5.5 Minimum Weight Spanning Trees 1.6 Sorting Revisited 1.6.1 Odd-Even Transposition Sort on a Linear Array 1.6.2 A Simple Root-N(log N + 1)-Step Sorting Algorithm 1.6.3 A (3 Root- N + o(Root-N))-Step Sorting Algorithm 1.6.4 A Matching Lower Bound 1.7 Packet Routing 1.7.1 Greedy Algorithms 1.7.2 Average-Case Analysis of Greedy Algorithms -Routing N Packets to Random Destinations -Analysis of Dynamic Routing Problems 1.7.3 Randomized Routing Algorithms 1.7.4 Deterministic Algorithms with Small Queues 1.7.5 An Off-line Algorithm 1.7.6 Other Routing Models and Algorithms 1.8 Image Analysis and Computational Geometry 1.8.1 Component-Labelling Algorithms -Levialdi's Algorithm -An O (Root-N)-Step Recursive Algorithm 1.8.2 Computing Hough Transforms 1.8.3 Nearest-Neighbor Algorithms 1.8.4 Finding Convex Hulls 1.9 Higher-Dimensional Arrays 1.9.1 Definitions and Properties 1.9.2 Matrix Multiplication 1.9.3 Sorting 1.9.4 Packet Routing 1.9.5 Simulating High-Dimensional Arrays on Low-Dimensional Arrays 1.10 problems 1.11 Bibliographic Notes 2 Meshes of Trees 2.1 The Two-Dimensional Mesh of Trees 2.1.1 Definition and Properties 2.1.2 Recursive Decomposition 2.1.3 Derivation from KN,N 2.1.4 Variations 2.1.5 Comparison With the Pyramid and Multigrid 2.2 Elementary O(log N)-Step Algorithms 2.2.1 Routing 2.2.2 Sorting 2.2.3 Matrix-Vector Multiplication 2.2.4 Jacobi Relaxation 2.2.5 Pivoting 2.2.6 Convolution 2.2.7 Convex Hull 2.3 Integer Arithmetic 2.3.1 Multiplication 2.3.2 Division and Chinese Remaindering 2.3.3 Related Problems -Iterated Products -Rooting Finding 2.4 Matrix Algorithms 2.4.1 The Three-Dimensional Mesh of Trees 2.4.2 Matrix Multiplication 2.4.3 Inverting Lower Triangular Matrices 2.4.4 Inverting Arbitrary Matrices -Csanky's Algorithm -Inversion by Newton Iteration 2.4.5 Related Problems 2.5 Graph Algorithms 2.5.1 Minimum-Weight Spanning Trees 2.5.2 Connected Components 2.5.3 Transitive Closure 2.5.4 Shortest Paths 2.5.5 Matching Problems 2.6 Fast Evaluation of Straight-Line Code 2.6.1 Addition and Multiplication Over a Semiring 2.6.2 Extension to Codes with Subtraction and Division 2.6.3 Applications 2.7 Higher-Dimensional meshes of Trees 2.7.1 Definitions and Properties 2.7.2 The Shuffle-Tree Graph 2.8 Problems 2.9 Bibliographic Notes 3 Hypercubes and Related Networks 3.1 The Hypercube 3.1.1 Definitions and Properties 3.1.2 Containment of Arrays -Higher-Dimensional Arrays -Non-Power-of-2 Arrays 3.1.3 Containment of Complete Binary Trees 3.1.4 Embeddings of Arbitrary Binary Trees -Embeddings with Dilation 1 and Load O(M over N + log N) -Embeddings with Dilation O(1) and Load O (M over N + 1) -A Review of One-Error-Correcting Codes -Embedding Plog N into Hlog N 3.1.5 Containment of Meshes of Trees 3.1.6 Other Containment Results 3.2 The Butterfly, Cube-Connected-Cycles , and Benes Network 3.2.1 Definitions and Properties 3.2.2 Simulation of Arbitrary Networks 3.2.3 Simulation of Normal Hypercube Algorithms 3.2.4 Some Containment and Simulation Results 3.3 The Shuffle-Exchange and de Bruijn Graphs 3.3.1 Definitions and Properties 3.3.2 The Diaconis Card Tricks 3.3.3 Simulation of Normal Hypercube Algorithms 3.3.4 Similarities with the Butterfly 3.3.5 Some Containment and Simulation Results 3.4 Packet-Routing Algorithms 3.4.1 Definitions and Routing Models 3.4.2 Greedy Routing Algorithms and Worst-Case Problems 3.4.3 Packing, Spreading, and Monotone Routing Problems -Reducing a Many-to-Many Routing Problem to a Many-to-One Routing Problem -Reducing a Routing Problem to a Sorting Problem 3.4.4 The Average-Case Behavior of the Greedy Algorithm -Bounds on Congestion -Bounds on Running Time -Analyzing Non-Predictive Contention-Resolution Protocols 3.4.5 Converting Worst-Case Routing Problems into Average-Case Routing Problems -Hashing -Randomized Routing 3.4.6 Bounding Queue Sizes -Routing on Arbitrary Levelled Networks 3.4.7 Routing with Combining 3.4.8 The Information Dispersal Approach to Routing -Using Information Dispersal to Attain Fault-Tolerance -Finite Fields and Coding Theory 3.4.9 Circuit-Switching Algorithms 3.5 Sorting 3.5.1 Odd-Even Merge Sort -Constructing a Sorting Circuit with Depth log N(log N +1)/2 3.5.2 Sorting Small Sets 3.5.3 A Deterministic O(log N log log N)-Step Sorting Algorithm 3.5.4 Randomized O(log N)-Step Sorting Algorithms -A Circuit with Depth 7.45 log N that Usually Sorts 3.6 Simulating a Parallel Random Access Machine 3.6.1 PRAM Models and Shared Memories 3.6.2 Randomized Simulations Based on Hashing 3.6.3 Deterministic Simulations using Replicated Data 3.6.4 Using Information Dispersal to Improve Performance 3.7 The Fast Fourier Transform 3.7.1 The Algorithm 3.7.2 Implementation on the Butterfly and Shuffle-Exchange Graph 3.7.3 Application to Convolution and Polynomial Arithmetic 3.7.4 Application to Integer Multiplication 3.8 Other Hypercubic Networks 3.8.1 Butterflylike Networks -The Omega Network -The Flip Network -The Baseline and Reverse Baseline Networks -Banyan and Delta Networks -k-ary Butterflies 3.8.2 De Bruijn-Type Networks -The k-ary de Bruijn Graph -The Generalized Shuffle-Exchange Graph 3.9 Problems 3.10 Bibliographic Notes Bibliography Index Lemmas, Theorems, and Corollaries Author Index Subject Index

De novo assembly and genotyping of variants using colored de Bruijn graphs

Abstract: Gil McVean and colleagues report algorithms for de novo assembly and genotyping of variants using colored de Bruijn graphs and provide these in a software implementation called Cortex. Their methods can detect and genotype both simple and complex genetic variants in either an individual or a population.

How to apply de Bruijn graphs to genome assembly

Abstract: A mathematical concept known as a de Bruijn graph turns the formidable challenge of assembling a contiguous genome from billions of short sequencing reads into a tractable computational problem.

MetaVelvet: an extension of Velvet assembler to de novo metagenome assembly from short sequence reads

Abstract: An important step in 'metagenomics' analysis is the assembly of multiple genomes from mixed sequence reads of multiple species in a microbial community. Most conventional pipelines use a single-genome assembler with carefully optimized parameters. A limitation of a single-genome assembler for de novo metagenome assembly is that sequences of highly abundant species are likely misidentified as repeats in a single genome, resulting in a number of small fragmented scaffolds. We extended a single-genome assembler for short reads, known as 'Velvet', to metagenome assembly, which we called 'MetaVelvet', for mixed short reads of multiple species. Our fundamental concept was to first decompose a de Bruijn graph constructed from mixed short reads into individual sub-graphs, and second, to build scaffolds based on each decomposed de Bruijn sub-graph as an isolate species genome. We made use of two features, the coverage (abundance) difference and graph connectivity, for the decomposition of the de Bruijn graph. For simulated datasets, MetaVelvet succeeded in generating significantly higher N50 scores than any single-genome assemblers. MetaVelvet also reconstructed relatively low-coverage genome sequences as scaffolds. On real datasets of human gut microbial read data, MetaVelvet produced longer scaffolds and increased the number of predicted genes.