Dendrobium fimbriatum

Abstract: Species identification of five Dendrobium plants was conducted using phylogenetic analysis and the validity of the method was verified. Some Dendrobium plants (Orchidaceae) have been used as herbal medicines but the difficulty in identifying their botanical origin by traditional methods prevented their full modern utilization. Based on the emerging field of molecular systematics as a powerful classification tool, a phylogenetic analysis was conducted using sequences of two plastid genes, the maturase-coding gene (matK) and the large subunit of ribulose 1,5-bisphosphate carboxylase-coding gene (rbcL), as DNA barcodes for species identification of Dendrobium plants. We investigated five medicinal Dendrobium species, Dendrobium fimbriatum, D. moniliforme, D. nobile, D. pulchellum, and D. tosaense. The phylogenetic trees constructed from matK data successfully distinguished each species from each other. On the other hand, rbcL, as a single-locus barcode, offered less species discriminating power than matK, possibly due to its being present with little variation. When results using matK sequences of D. officinale that was deposited in the DNA database were combined, D. officinale and D. tosaense showed a close genetic relationship, which brought us closer to resolving the question of their taxonomic identity. Identification of the plant source as well as the uniformity of the chemical components is critical for the quality control of herbal medicines and it is important that the processed materials be validated. The methods presented here could be applied to the analysis of processed Dendrobium plants and be a promising tool for the identification of botanical origins of crude drugs.

Abstract: population. Plants have played a significant role in maintaining human health and improving the quality of human life for thousands of years and have served humans as well as valuable source of new natural products. Despite the availability of different approaches for the discovery of therapeutics, plant products still remain as one of the best reservoirs of new structural types. About 25% of all prescriptions sold in the United States are from natural products (Farnsworth, 1990). Orchidaceae is a highly evolved and widely distributed monocotyledonous family with a large number of terrestrial, saprophytic and epiphytic species. It comprises more than 30, 000 species in approximately 750 genera (Kong et al., 2003), and new ones are being discovered by almost every botanical expedition in tropical areas. Many orchids are used in traditional system of medicine as a remedy for a number of ailments. The tubers and pseudobulbs of several orchids like Orchis latifolia, Orchis mascula, Cymbidium aloifolium, Zeuxine strateumatica, and some species of Dendrobium, Eulophia and Habenaria are used as a restorative and in the treatment of various diseases (Puri, 1970). The seeds of Cymbidium aloifolium used for healing of wounds. The powdered roots of Vanda tessellate is considered as antidote for poisoning. It is also used in rheumatic pains and abdominal complaints. Dendrobium fimbriatum has been used for liver upsets and nervous debility, while Dendrobium teretifolium for headache and pain reliever in other parts of body. Other orchid genera like Oberonia, Eria, Bulbophyllum, Eulophia, Geodorum, Grammatophylum, and Hetaeria are also reported to be used as medicine in different parts of the world to cure various diseases (Hawkes, 1944; Withner et al., 1974). Phytochemical investigations of the orchid family were performed for alkaloid constituents (, 1974),

Abstract: Dendrobium is a large genus of tropical epiphytic orchids. Some members of this genus are in danger of extinction across China. To investigate orchid mycorrhizal associations of the genus Dendrobium, plants from two Dendrobium species (Dendrobium officinale and Dendrobium fimbriatum) were collected from two habitats in Guangxi Province, China, and clone libraries were constructed to identify the mycorrhizal fungi of individual plants. A low and high degree of specificity was observed in D. officinale and D. fimbriatum, respectively. Phylogenetic analysis revealed that the majority of Dendrobium mycorrhizal fungi are members of the Tulasnellaceae, but, in some plants, members of the Ceratobasidiaceae and Pluteaceae were also found. In D. officinale, individual plants associated with more than three fungi simultaneously, and, in some cases, associations with five fungi at the same time. One fungus was shared by individual plants of D. officinale collected from the two habitats. In D. fimbriatum, only one fungal partner was found in each population, and this fungus differed between populations. The two species of Dendrobium sampled from the same habitat did not share any fungal taxa. These results provide valuable information for conservation of these orchid species.

Abstract: The bioassay-guided chemical investigation of the stems of Dendrobium fimbriatum Hook led to the isolation of seven first reported bibenzyl dimers with a linkage of a methylene moiety, fimbriadimerbi-benzyls A-G (1-7), together with a new dihydrophenanthrene derivative (S)-2,4,5,9-tetrahydroxy-9, 10-dihydrophenanthrene (8) and thirteen known compounds (9-21). The structure of the new compound was established by spectroscopic analysis. Biological evaluation of bibenzyl derivatives against five human cell lines indicated that seven of those compounds exhibited broad-spectrum and cytotoxic activities with IC50 values ranging from 2.2 to 21.2 mu M. Those rare bibenzyl dimers exhibited cytotoxic activities in vitro and the cytotoxicity decreased as the number of oxygen-containing groups in the structure decreases. (C) 2014 Elsevier Ltd. All rights reserved.

Abstract: Aim To investigate the chemical constituents of Dendrobium fimbriatum Hook Methods Various chromatographic techniques were employed for isolation and purification of the constituents The structures were elucidated by IR, MS, 1HNMR, 13CNMR and 2D-NMR Results Eight compounds were obtained and identified by spectral analysis as fimbriatone, confusarin, crepidatin, physcion, rhein, ayapin, scopolin methyl ether and n-octacostyl ferulate Conclusion Fimbriatone is a new compound, physcion and rhein are firstly isolated from Genus Dendrobium The others are found from this species for the first time Fimbriatone showed potential inhibitory effects on BGC cell line