Topic
Dengue fever
About: Dengue fever is a research topic. Over the lifetime, 17463 publications have been published within this topic receiving 485745 citations. The topic is also known as: Dengue & dengue disease.
Papers published on a yearly basis
Papers
More filters
••
TL;DR: A live-attenuated chimeric vaccine against JE virus is developed by replacing the premembrane and envelope genes of the YF 17D vaccine with the corresponding genes from SA 14-14-2 an attenuated JEirus strain to produce YF-JE chimera.
154 citations
•
TL;DR: The clinical findings in 206 patients with dengue fever (DF) or with d Dengue hemorrhagic fever (DHF) during the epidemic of 1996 at Lucknow are described.
Abstract: This paper describes the clinical findings in 206 patients with dengue fever (DF) or with dengue hemorrhagic fever (DHF) during the epidemic of 1996 at Lucknow. The age group affected most was 11 to 30 years and 21% of the patients were less than 10 years old. The male:female ratio was 1.9:1. The onset was abrupt in all the patients, severe frontal headache was observed in 97%, myalgia in 90%, skin rash in 40%, vomiting in 29% and arthralgia in knee and hip joints in 9%. Anuria was seen in two patients. Lymphadenopathy was noted in 14%, hepatomegaly in 4%, being associated with mild jaundice in one patient, and splenomegaly in 2% of the patients. Involvement of the heart and lungs was seen in one patient each and no case with encephalitis was recorded. Hemorrhages from various sites were observed in 54% patients and 17 patients had profound shock. The commonest bleeding site was gums. Profound shock was preceded by various warning signs, the commonest being sudden hypotension. Among the patients with profound shock the mortality was 47% while the overall fatality rate was 3.8%. A number of the risk factors existed for a long time in this part of the world, but what precipitated the present epidemic at this time, is not known.
154 citations
••
TL;DR: Rhesus macaques inoculated intravenously with a high dose of d Dengue virus produced dengue hemorrhage, which may provide a unique platform to define the early events in dengingue virus infection and help identify which blood components contribute to the pathogenesis of denge disease.
154 citations
••
TL;DR: Intracellular dengue virus RNA from cells infected with transcript-derived virus contained an introduced BstEII site, proving that infectivity was derived from RNA transcripts and not from contamination with parental d Dengue virus.
Abstract: The dengue virus type 2 genomic RNA was amplified by reverse transcription-PCR and cloned as four cDNA fragments. We could not assemble these four fragments into full-length cDNA in Escherichia coli. The full-length dengue virus cDNA was constructed by homologous recombination in yeast, either as part of a yeast artificial chromosome or in a yeast-E. coli shuttle vector. Full-length cDNA clones were propagated once in E. coli to prepare useful quantities of DNA. In vitro transcription of these clones produced full-length RNA transcripts. Introduction of these transcripts into LLC-MK2 cells produced typical dengue infection, as judged by cytopathic effects and indirect immunofluorescence. Infectivity was sensitive to RNase digestion and was dependent on the presence of cap analog in the transcription reaction mixture. Virus in the medium was passaged on C6-36 cells to produce stocks, and these stocks had titers and plaque morphologies similar to those of the parental dengue virus type 2. Intracellular dengue virus RNA from cells infected with transcript-derived virus contained an introduced BstEII site, proving that infectivity was derived from RNA transcripts and not from contamination with parental dengue virus. Transcript-derived virus was comparable to dengue virus type 2 for growth and protein expression in tissue culture cells. Sequence analysis of the dengue virus cDNA in one full-length clone revealed only one unexpected silent mutation. By using yeast technology, it will be easy to introduce specific mutations into the dengue virus cDNA, allowing analysis of the virus phenotype in cells transfected with mutant transcripts.
153 citations
••
TL;DR: Virologic, serologic, and clinical responses to infection were studied in monkeys inoculated with dengue 1-4 viruses and no abnormalities were observed in serial hematocrit, prothrombin time, and determinations of total protein.
Abstract: Virologic, serologic, and clinical responses to infection were studied in 122 Macaca mulatta monkeys and 17 monkeys of three other species that were inoculated with dengue 1-4 viruses passaged in tissue culture. Susceptible rhesus monkeys, inoculated with either high (1037-10".pfu) or low (8-50 pfu) doses of virus always developed antibody. Frequently with dengue 2 infection, but less frequently with dengue 1 infection, lymphadenomegaly, depression of leukocyte count, and lymphocytosis were noted. In approximately 90% of infected animals viremia began two to six days after inoculation; 90% of dengue 2 and 4 viremias lasted six days or less; the average duration of dengue 1 viremia was somewhat longer, and of dengue 3 viremia shorter than this. HAI titers to the homologous antigen in convalescent sera were usually twofold higher than titers to heterologous dengue viruses; antibody response to dengue 4 infection was relatively specific. No abnormalities were observed in serial hematocrit, prothrombin time, and determinations of total protein. Levels of complement in serum rose several days after the start of serial bleedings in both infected and control animals. The courses of infection due to dengue viruses are similar in humans and monkeys.
153 citations