Topic
Dengue virus
About: Dengue virus is a research topic. Over the lifetime, 12671 publications have been published within this topic receiving 461406 citations. The topic is also known as: DENV.
Papers published on a yearly basis
Papers
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TL;DR: Cross neutralization tests using monkey antiserum confirmed the results of the one-way neutralization test using antisersum to reference dengue strains and also indicated antigenic variation within serotypes 1, 2 and 4.
Abstract: Cross neutralization tests using monkey antiserum confirmed the results of the one-way neutralization test using antiserum to reference dengue strains and also indicated antigenic variation within serotypes 1, 2 and 4.
189 citations
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TL;DR: In vivo protection studies with neem leaves extract at its maximum non-toxic concentrations 120-30 mg/ml resulted in inhibition of the virus replication as confirmed by the absence of Dengue related clinical symptoms in suckling mice and absence of virus specific 511 bp amplicon in RT-PCR.
189 citations
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TL;DR: Compound 6 was identified as one of the inhibitors and had an average 50% effective concentration of 119 nM against dengue virus serotype 2 in a human cell line and Mechanism-of-action studies demonstrated that compound 6 acts at an early stage during d Dengue virus infection.
Abstract: The incidence of dengue fever epidemics has increased dramatically over the last few decades. However, no vaccine or antiviral therapies are available. Therefore, the need for safe and effective antiviral drugs has become imperative. The entry of dengue virus into a host cell is mediated by its major envelope (E) protein. The crystal structure of the E protein reveals a hydrophobic pocket that is presumably important for low-pH-mediated membrane fusion. High-throughput docking with this hydrophobic pocket was performed, and hits were evaluated in cell-based assays. Compound 6 was identified as one of the inhibitors and had an average 50% effective concentration of 119 nM against dengue virus serotype 2 in a human cell line. Mechanism-of-action studies demonstrated that compound 6 acts at an early stage during dengue virus infection. It arrests dengue virus in vesicles that colocalize with endocytosed dextran and inhibits NS3 expression. The inhibitors described in this report can serve as molecular probes for the study of the entry of flavivirus into host cells.
189 citations
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TL;DR: The dual sites of action of an HS-like molecule suggest that, at least in monkey kidney and human hepatic cells, the HS residues in the cell membrane appear to act as mediators for DENV-2 entry, an interesting alternative target for flavivirus therapy.
188 citations
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TL;DR: An NS3-specific antiserum is used, under nondenaturing conditions, to demonstrate that NS2B and NS3 form a complex both in vitro and in vivo, and a potential conserved cleavage site that resembles other sites cleaved by the NS3/NS2B proteinase is revealed.
188 citations