Topic
Detection limit
About: Detection limit is a research topic. Over the lifetime, 34379 publications have been published within this topic receiving 644817 citations. The topic is also known as: limit of detection & lower detection limit.
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TL;DR: A sensitive and selective electrochemical biosensor is developed for the determination of chloramphenicol exploring its direct electron transfer processes in in-vitro model and pharmaceutical samples and a significant accumulation by the drug sensitive H. influenza strain is observed for the first time in this study using a biosensor.
104 citations
TL;DR: A lateral flow nucleic acid biosensor based on copper-dependent DNA-cleaving DNAzyme and gold nanoparticles has been developed for the visual detection of copper ions in an aqueous solution with a detection limit of 10 nM.
104 citations
TL;DR: In this paper, a simple, low cost, and green method was developed for the synthesis of water-soluble and well-dispersed fluorescent carbon nanodots (CDs) via a one-step hydrothermal treatment of potatoes.
Abstract: A simple, low cost, and green method was developed for the synthesis of water-soluble and well-dispersed fluorescent carbon nanodots (CDs) via a one-step hydrothermal treatment of potatoes. The as-prepared CDs exhibit a strong blue fluorescence, with a quantum yield of up to 15%. We further explored the use of these CDs as a novel sensing probe for the label-free, sensitive and selective detection of Fe3+. This is based on strong fluorescence quenching due to the complex formed between the CDs and Fe3+. The detection limit was as low as 0.025 μmol L−1, and different concentrations corresponded to different sensitivities. The linear ranges were 1.0–5.0 μmol L−1 and 5.0–50.0 μmol L−1 at lower and higher concentration ranges respectively, and the recoveries of the spiked water samples were 93.7–101.5%. Therefore, the as-prepared CDs could meet the requirements for the monitoring of Fe3+ in environmental samples.
104 citations
TL;DR: High-affinity anti-abrin-a monoclonal and polyclonal antibodies were used to develop a sandwich immunochromatographic assay and silver enhancement technology was used to further increase the sensitivity.
104 citations
TL;DR: A label-free fluorometric sensor for Cu(2+) sensing was developed based on the unique multivalent metal ion-binding property of H39GFP and fluorescence quenching effect of Cu( 2+) by electron transfer.
Abstract: Protein engineering by resurfacing is an efficient approach to provide new molecular toolkits for biotechnology and bioanalytical chemistry. H39GFP is a new variant of green fluorescent protein (GFP) containing 39 histidine residues in the primary sequence that was developed by protein resurfacing. Herein, taking H39GFP as the signal reporter, a label-free fluorometric sensor for Cu2+ sensing was developed based on the unique multivalent metal ion-binding property of H39GFP and fluorescence quenching effect of Cu2+ by electron transfer. The high affinity of H39GFP with Cu2+ (Kd, 16.2 nM) leads to rapid detection of Cu2+ in 5 min with a low detection limit (50 nM). Using acetylthiocholine (ATCh) as the substrate, this H39GFP/Cu2+ complex-based sensor was further applied for the turn-on fluorescence detection of acetylcholinesterase (AChE) activity. The assay was based on the reaction between Cu2+ and thiocholine, the hydrolysis product of ATCh by AChE. The proposed sensor is highly sensitive (limit of dete...
104 citations