scispace - formally typeset
Search or ask a question
Topic

Detection limit

About: Detection limit is a research topic. Over the lifetime, 34379 publications have been published within this topic receiving 644817 citations. The topic is also known as: limit of detection & lower detection limit.


Papers
More filters
Journal ArticleDOI
TL;DR: Such combination of a complete integrated immunoassay, an attractive analytical performance, and the distinct miniaturization/portability advantages of electrochemical microsystems offers considerable promise for designing self-contained and disposable chips for decentralized clinical diagnostics or on-site environmental testing.
Abstract: A microfluidic device for conducting electrochemical enzyme immunoassays is described. The new "lab-on-a-chip" protocol integrates precolumn reactions of alkaline phosphatase-labeled antibody (anti-mouse IgG) with the antigen (mouse IgG), followed by electrophoretic separation of the free antibody and antibody-antigen complex. The separation is followed by a postcolumn reaction of the enzyme tracer with the 4-aminophenyl phosphate substrate and a downstream amperometric detection of the liberated 4-aminophenol product Factors influencing the reaction, separation, and detection processes were optimized, and the analytical performance was characterized. An applied field strength of 256 V/cm results in free antibody and antibody-antigen complex migration times of 125 and 340 s, respectively. A remarkably low detection limit of 2.5 x 10(-16) g/mL (1.7 x 10(-18) M) is obtained for the mouse IgG model analyte. Such combination of a complete integrated immunoassay, an attractive analytical performance, and the distinct miniaturization/portability advantages of electrochemical microsystems offers considerable promise for designing self-contained and disposable chips for decentralized clinical diagnostics or on-site environmental testing.

180 citations

Journal ArticleDOI
15 Nov 2013-Talanta
TL;DR: The carbon dots obtained shows a strong emission at the wavelength of 400 nm with an optimum excitation of 305 nm and the fluorescence of the carbon dots was significantly quenched particularly by lead (II) ions in a selective manner.

180 citations

Journal ArticleDOI
13 Sep 2001-Talanta
TL;DR: A sensor for H( 2)O(2) amperometric detection based on a Prussian Blue bulk modified carbon screen-printed electrode was developed and has been optimised with respect to the lowest limit of detection achieved.

180 citations

Journal ArticleDOI
TL;DR: The results demonstrate that multiplexed immuno-MRM-MS can be made reproducible across independent laboratories and has the potential to be adopted widely for assaying proteins in matrices as complex as plasma.

178 citations

Journal ArticleDOI
TL;DR: The analytical performance and degree of automation of the glycerol method makes it well suited for serial studies oflipolysis in human fat cells in the presence of lipolytic or antilipolytic agents.

178 citations


Network Information
Related Topics (5)
Mass spectrometry
72.2K papers, 2M citations
90% related
High-performance liquid chromatography
47.3K papers, 1M citations
90% related
Cyclic voltammetry
55.9K papers, 1.4M citations
84% related
Aqueous solution
189.5K papers, 3.4M citations
84% related
Reagent
60K papers, 1.2M citations
83% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20241
20236,071
202212,796
20211,671
20201,442
20191,445