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Detection limit

About: Detection limit is a research topic. Over the lifetime, 34379 publications have been published within this topic receiving 644817 citations. The topic is also known as: limit of detection & lower detection limit.


Papers
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Journal ArticleDOI
TL;DR: In this paper, a reagent-injection chemiluminescent method for the determination of hydrogen peroxide (H2O2) has been developed, which is based on the catalytic (cobalt (II)) oxidation of luminol by H2O 2 in an alkaline solution.
Abstract: A reagent-injection chemiluminescent method for the determination of hydrogen peroxide (H2O2) has been developed. The method is based on the catalytic (cobalt (II)) oxidation of luminol by hydrogen peroxide in an alkaline solution. One mixed reagent is used for the analysis using the reagent injection method. The mixed reagent is optimized for pH and concentration of luminol and Co2+. Apart from Fe2+, none of the 14 species tested showed interference at their seawater levels. Because Fe2+ oxidation is rapid compared to the rate of decay of hydrogen peroxide, the Fe2+ interference can be eliminated by storing seawater samples for 1 h. The combination of reagents and the adaptation of reagent injection lead to a subnanomolar detection limit. The accuracy of the method is at least 0.42 nM. The precision of the method is 17 pM (for a 0.57 nM sample). The method has been used aboard ship to determine the concentrations of hydrogen peroxide in seawater samples in the open ocean as well as on the continental shelf.

167 citations

Journal ArticleDOI
TL;DR: In this article, peroxyoxalate chemiluminescence is applied to the high-performance liquid chromatographic detection of polycyclic aromatic hydrocarbons (PAH), and the method yields linear responses to PAH over 3 orders of magnitude, and in some cases detection limits are better than those determined by fluorescence using the same fluorometer.
Abstract: Peroxyoxalate chemiluminescence is applied to the high-performance liquid chromatographic detection of polycyclic aromatic hydrocarbons (PAH). PAH are excited by energy transfer from the decomposition products of the reaction between hydrogen peroxide and bis(2,4,6-trichlorophenyl) oxalate. These reagents are introduced by postcolumn mixing, and the emission is observed by using a conventional fluorescence detector with its source turned off. The method yields linear responses to PAH over 3 orders of magnitude, and in some cases detection limits are better than those determined by fluorescence using the same fluorometer. Chemiluminescence detection is compared to ultraviolet absorbance and fluorescence detection of PAH in terms of sensitivity and selectivity.

167 citations

Journal ArticleDOI
TL;DR: A flow cytometer system was used to detect aqueous rhodamine 6G by laser-induced fluorescence and the results presented here allow a projection to single-molecule detection with reasonable improvements to the apparatus.
Abstract: A flow cytometer system was used to detect aqueous rhodamine 6G by laser-induced fluorescence. Best results were obtained with careful spectral and spatial filtering. At the detection limit, the probability of a rhodamine 6G molecule being present in the detector's probed volume of 11 pL is about 0.6 . With a flow rate of 0.42 ..mu..L/s, a detection limit of 8.9 x 10/sup -14/ M was obtained for a 1-s time constant. At the detection limit, 18 ag or 22,000 molecules of rhodamine 6G flowed through the probed volume during the signal integration period. Signal linearity extends over greater than 5 orders of magnitude limited only by saturation of the detection electronics at high concentration. The results presented here allow a projection to single-molecule detection with reasonable improvements to the apparatus. 25 references, 5 figures, 7 tables.

167 citations

Journal ArticleDOI
TL;DR: A paired-ion, reversed-phase, high-performance liquid chromatography procedure using electrochemical detection and internal standard quantitation based on isoascorbic acid (IA), which overcomes a major problem associated with AA instability and eliminates the necessity of assaying samples immediately after they are prepared for analysis.

166 citations

Journal ArticleDOI
TL;DR: A solid-phase extraction (SPE) and liquid chromatographic (LC) method was developed for the determination of selected fluoroquinolone (FQ) drugs including ofloxacin, norfloxac in municipal wastewater samples.

166 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20241
20236,071
202212,796
20211,671
20201,442
20191,445