Topic
Detection limit
About: Detection limit is a research topic. Over the lifetime, 34379 publications have been published within this topic receiving 644817 citations. The topic is also known as: limit of detection & lower detection limit.
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TL;DR: In this article, a thiolated aptamer specific for AFB1 was linked to the surface of CdTe quantum dots via ligand exchange, and the fluorescence of modified-Q-dots was strongly quenched by GO.
Abstract: Aflatoxin B1 (AFB1), a secondary fungal metabolite of Aspergillus flavus, was employed as a model mycotoxin to establish an aptamer based assay that exploits the quenching of the fluorescence of CdTe quantum dots (Q-dots) by graphene oxide (GO). A thiolated aptamer specific for AFB1 was linked to the surface of Q-dots via ligand exchange. The fluorescence of the aptamer modified-Q-dots is strongly quenched by GO. If, however, AFB1 is added, fluorescence is restored depending on the quantity of AFB1 added. The system was evaluated both in phosphate buffer solution and in peanut oil. If performed in an aqueous system, the assay possesses good selectivity, a wide dynamic range (from 3.2 nM to 320 μM) and a low limit of detection (1.0 nM). If performed in peanut oil solution, the dynamic range is from 1.6 nM to 160 μM, and the limit of detection is 1.4 nM. In our perception, this is a simple, sensitive and selective method for the determination of AFB1 that also may be extended to the analysis of other mycotoxins.
134 citations
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TL;DR: The amounts of free iron were detected using a catalytic action of Fe(III) and Fe(II) on the oxidation of N,N-dimethyl-p-phenylenediamine in the presence of hydrogen peroxide.
134 citations
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TL;DR: A single-drop microextraction method was developed for separation and preconcentration of mercury species, which relies on the formation of the corresponding dithizonates and microextracted of these neutral chelates onto a microdrop of an ionic liquid.
134 citations
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TL;DR: The dispersive liquid-liquid microextraction (DLLME) was combined with the flame atomic absorption spectrometry (FAAS) for determination of lead in the water samples and some effective parameters on the micro Extraction and the complex formation were selected and optimized.
134 citations
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TL;DR: The attractive behavior of the new "mercury-free" chromium sensor holds great promise for on-site environmental and industrial monitoring of chromium (VI).
134 citations