Topic
Detection limit
About: Detection limit is a research topic. Over the lifetime, 34379 publications have been published within this topic receiving 644817 citations. The topic is also known as: limit of detection & lower detection limit.
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TL;DR: A sensitive and specific sandwich assay for human alpha-thrombin that takes advantage of sandwich binding of two affinity aptamers for increased specificity, gold nanoparticles for signal amplification, magnetic beads for fast magnetic separation, and inductively coupled plasma mass spectrometry for ultrasensitive detection.
Abstract: We describe a sensitive and specific sandwich assay for human α-thrombin. The assay takes advantage of sandwich binding of two affinity aptamers for increased specificity, gold nanoparticles for signal amplification, magnetic beads for fast magnetic separation, and inductively coupled plasma mass spectrometry for ultrasensitive detection. Other proteins, such as immunoglobulin G, serum albumin, transferrin, fibrinogen, and lysozyme did not show interference with the assay for human α-thrombin. The detection limit of human α-thrombin was as low as 0.5 fmol, corresponding to 10 pM thrombin in 50 μL, and the dynamic range covered ∼3 orders of magnitude.
128 citations
TL;DR: An improved method is described, making use of separation by high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD) for quantification of primary sugars, sugar alcohols and anhydrosugars in atmospheric aerosols, well suited to handling large numbers of samples.
127 citations
TL;DR: In this paper, a simple and highly selective flow injection (FI) on-line preconcentration and separation flame atomic absorption spectrometric (FAAS) method was developed for the determinations of trace amounts of silver, gold and palladium.
127 citations
TL;DR: An ion-pair liquid chromatography method with on-line photodiode-array and electrospray mass spectrometry detection was developed and demonstrated by analyzing sulphonated azo dyes in soft drinks, fruit jam, and salted vegetables.
127 citations
TL;DR: A capillary electrophoresis method with ultraviolet (UV) detection was developed and optimized for the enantiomer separation of norepinephrine, epinephrine and isoprenaline using dual cyclodextrins of 2-hydroxypropyl-beta-CD and heptakis as chiral selectors.
127 citations