Topic
Diphenylcarbazide
About: Diphenylcarbazide is a research topic. Over the lifetime, 288 publications have been published within this topic receiving 5403 citations. The topic is also known as: diphenylcarbazide.
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TL;DR: The extraction medium used contains chloroform-heptane-methanol mixture with a phosphate buffer gives a sufficient extraction of FFA from serum and contamination with interfering agents is avoided.
655 citations
TL;DR: A colorimetric micromethod, based on the formation of FFA-Cu soaps, was developed for determining free fatty acids (FFA) in 50 μl of plasma, which requires only capillary samples and is suitable for repeated registration of the concentration of F FA during experimental conditions.
432 citations
TL;DR: In this article, an analysis of pH influence, particle size, adsorbent weight, concentration and oxidation state of metal was carried out on cross-linked chitosan.
230 citations
TL;DR: The use of diphenylcarbazide as an electron donor coupled to the photoreduction of 2,6-dichlorophenolindophenol by tris-washed chloroplasts or subchloroplast fragments provides a simple and sensitive assay for photosystem 2 of chloroplast.
Abstract: The use of diphenylcarbazide as an electron donor coupled to the photoreduction of 2,6-dichlorophenolindophenol by tris-washed chloroplasts or subchloroplast fragments provides a simple and sensitive assay for photosystem 2 of chloroplasts. By varying the concentration of tris buffer at pH 8.0 during an incubation period it is shown that the destruction of oxygen evolution activity is accompanied by a corresponding emergence of an ability to photooxidize diphenylcarbazide, as evidenced by absorbance changes due to diphenylcarbazide at 300 nm. The temperature-sensitive oxidation of diphenylcarbazide is inhibited by DCMU and by high ionic strengths. This activity appears to measure the primary photochemical reaction of photosystem 2.
187 citations
TL;DR: The proposed method offers the sensitivity, convenience, and accuracy needed for the determination of FFA in situations where the size of sample available is limiting, as with small rodents.
181 citations