Divalent

About: Divalent is a research topic. Over the lifetime, 8154 publications have been published within this topic receiving 234108 citations. The topic is also known as: bivalent.

LTRPC7 is a Mg·ATP-regulated divalent cation channel required for cell viability

Abstract: The molecular mechanisms that regulate basal or background entry of divalent cations into mammalian cells are poorly understood. Here we describe the cloning and functional characterization of a Ca2+- and Mg2+-permeable divalent cation channel, LTRPC7 (nomenclature compatible with that proposed in ref. 1), a new member of the LTRPC family of putative ion channels. Targeted deletion of LTRPC7 in DT-40 B cells was lethal, indicating that LTRPC7 has a fundamental and nonredundant role in cellular physiology. Electrophysiological analysis of HEK-293 cells overexpressing recombinant LTRPC7 showed large currents regulated by millimolar levels of intracellular Mg.ATP and Mg.GTP with the permeation properties of a voltage-independent divalent cation influx pathway. Analysis of several cultured cell types demonstrated small magnesium-nucleotide-regulated metal ion currents (MagNuM) with regulation and permeation properties essentially identical to the large currents observed in cells expressing recombinant LTRPC7. Our data indicate that LTRPC7, by virtue of its sensitivity to physiological Mg.ATP levels, may be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell.

The role of divalent cations in the N-methyl-D-aspartate responses of mouse central neurones in culture.

Abstract: 1. Single-channel currents activated by N-methyl-D-aspartate (NMDA) agonists were analysed in the presence of various extracellular concentrations of divalent cations in outside-out patches from mouse neurones in primary culture. 2. In nominally Mg2+-free solutions the opening and closing of the channels leads to rectangular current pulses, the mean duration of which varies little with membrane potential. After addition of Mg2+, the single-channel currents recorded at negative potentials appear in bursts of short openings separated by brief closures. 3. The duration of the short openings decreases with increasing Mg2+ concentration, while the duration of the short closures is independent of the Mg2+ concentration. Depolarization increases the duration of the short openings and decreases the duration of the short closures. 4. The dependence of the burst structure on the Mg2+ concentration and on membrane potential is compatible to a first approximation with a model in which Mg2+ ions enter the open channel and block it by binding at a deep site. A better approximation requires, however, additional assumptions such as Mg2+ permeation and/or interactions between Ca2+ and Mg2+. 5. Increasing the extracellular Ca2+ concentration from 1 to 100 mM produces three effects on the currents flowing through NMDA channels. It shifts the reversal potential towards a positive value (+30 mV); it reduces the outward current flowing through the NMDA channels at very positive potentials; it reduces the inward current flowing at negative potentials. 6. The interpretation of the effects of Ca2+ appears to require three hypotheses: that Ca2+ permeates the NMDA channel, that there exists a significant surface potential at the entrance of the NMDA channel in physiological solutions and that both Ca2+ and monovalent cations bind to the channel, binding being stronger in the case of Ca2+ ions. 7. While Co2+ and, to a lesser extent, Mn2+ mimic the effects of Mg2+ on the NMDA channel, Ca2+, Ba2+ and Cd2+ do not. The distinction between Mg2+-like and Ca2+-like divalent cations corresponds to a difference in the speed of exchange of the water molecules surrounding the cations in solutions. Thus, it is possible that permeation occurs for all the divalent cations, but is slower for those which are slowly dehydrated.

Luminescent Transitions Associated With Divalent Copper Impurities and the Green Emission from Semiconducting Zinc Oxide

Abstract: Luminescent transitions at divalent copper impurities in ZnO give rise to a close-spaced, no-phonon doublet at \ensuremath{\sim}2.8590 eV, as well as a broad multiphonon side band. The transition is found to be an optical charge transfer between a highly shielded localized level of the copper impurity and a level which is strongly perturbed by the valence-band states of the crystal. The analysis provides information about the role of deep acceptor states in electronic processes in covalent solids.