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DNA

About: DNA is a research topic. Over the lifetime, 107156 publications have been published within this topic receiving 4706496 citations. The topic is also known as: deoxyribonucleic acid & deoxyribose nucleic acid.


Papers
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Journal ArticleDOI
TL;DR: In this paper, a procedure for extracting plasmid DNA from bacterial cells is described, which is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day, yet yields DNA which is pure enough to be digestible by restriction enzymes.
Abstract: A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes. The principle of the method is selective alkaline denaturation of high molecular weight chromosomal DNA while covalently closed circular DNA remains double-stranded. Adequate pH control is accomplished without using a pH meter. Upon neutralization, chromosomal DNA renatures to form an insoluble clot, leaving plasmid DNA in the supernatant. Large and small plasmid DNAs have been extracted by this method.

13,805 citations

Journal ArticleDOI
Julius Marmur1
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.

11,573 citations

Journal ArticleDOI
TL;DR: Labeled DNAs (and restriction endonuclease fragments derived from them) are useful probes for detecting rare homologous sequences by in situ hybridization and reassociation kinetic analysis.

10,489 citations

Journal ArticleDOI
TL;DR: A method is presented for the rapid isolation of high molecular weight plant DNA which is free of contaminants which interfere with complete digestion by restriction endonucleases, and which yields total cellular DNA.
Abstract: A method is presented for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length) which is free of contaminants which interfere with complete digestion by restriction endonucleases. The procedure yields total cellular DNA (i.e. nuclear, chloroplast, and mitochondrial DNA). The technique is ideal for the rapid isolation of small amounts of DNA from many different species and is also useful for large scale isolations.

10,481 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20251
20234,676
20226,593
20212,407
20202,527
20192,567