Showing papers on "DPPH published in 1979"
Patent•
09 Oct 1979
TL;DR: In this article, a small amount of eithanol is added to the crude extract, and insoluble substance is isolated by carrying out thin-layer chromatography three times three times.
Abstract: NEW MATERIAL:Heterocyclic phenol compound AOS Elemental analysis: C 6264, H 607, N 562, O 2568%; molecular weight: 284; mp: 1246-1254 degC; solubility: slightly soluble in water, insoluble in n-hexane and easily soluble in ether and ethanol; color reaction: positive to nitrous acid, Rhodamine B, DPPH or PDAB; shows brown color with PNA or PNA and sodium carbonate, and blue to green with 50% sulfuric acid; color; light brown USE:Natural antioxidant of fats and oils, food, confectionery, etc containing thereof, etc PROCESS:Defatted safflower seeds are extracted with a polar organic solvent, to obtain crude extract (the yield is about 2%) A small amount of eithanol is added to the crude extract, and insoluble substance is isolated The filtrate is cooled, centrifuged, and-subjected to liquid column chromatography Then, AOS is isolated by carrying out thin-layer chromatography three times
3 citations