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Embryonic stem cell

About: Embryonic stem cell is a(n) research topic. Over the lifetime, 35174 publication(s) have been published within this topic receiving 1921313 citation(s). The topic is also known as: ES cells & ESC. more


Open accessJournal ArticleDOI: 10.1016/J.CELL.2006.07.024
Kazutoshi Takahashi1, Shinya Yamanaka1Institutions (1)
25 Aug 2006-Cell
Abstract: Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was dispensable. These cells, which we designated iPS (induced pluripotent stem) cells, exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Subcutaneous transplantation of iPS cells into nude mice resulted in tumors containing a variety of tissues from all three germ layers. Following injection into blastocysts, iPS cells contributed to mouse embryonic development. These data demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors. more

Topics: KOSR (74%), Induced pluripotent stem cell (72%), Stem cell (70%) more

21,746 Citations

Open accessJournal ArticleDOI: 10.1016/J.CELL.2007.11.019
Kazutoshi Takahashi1, Koji Tanabe1, Mari Ohnuki1, Megumi Narita1  +3 moreInstitutions (1)
30 Nov 2007-Cell
Abstract: SUMMARY Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generationofiPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts. more

Topics: Induced pluripotent stem cell (72%), Embryonic stem cell (71%), Stem cell (70%) more

16,624 Citations

Open accessJournal ArticleDOI: 10.1126/SCIENCE.282.5391.1145
06 Nov 1998-Science
Abstract: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages. After undifferentiated proliferation in vitro for 4 to 5 months, these cells still maintained the developmental potential to form trophoblast and derivatives of all three embryonic germ layers, including gut epithelium (endoderm); cartilage, bone, smooth muscle, and striated muscle (mesoderm); and neural epithelium, embryonic ganglia, and stratified squamous epithelium (ectoderm). These cell lines should be useful in human developmental biology, drug discovery, and transplantation medicine. more

Topics: Human embryonic stem cell line (74%), Embryoid body (67%), Embryonic Germ Cells (65%) more

14,753 Citations

Journal ArticleDOI: 10.1126/SCIENCE.1151526
21 Dec 2007-Science
Abstract: Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells. These induced pluripotent human stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such induced pluripotent human cell lines should be useful in the production of new disease models and in drug development, as well as for applications in transplantation medicine, once technical limitations (for example, mutation through viral integration) are eliminated. more

Topics: Induced pluripotent stem cell (70%), Embryonic stem cell (70%), Embryoid body (70%) more

9,307 Citations

Open accessJournal ArticleDOI: 10.1073/PNAS.78.12.7634
Abstract: This report describes the establishment directly from normal preimplantation mouse embryos of a cell line that forms teratocarcinomas when injected into mice. The pluripotency of these embryonic stem cells was demonstrated conclusively by the observation that subclonal cultures, derived from isolated single cells, can differentiate into a wide variety of cell types. Such embryonic stem cells were isolated from inner cell masses of late blastocysts cultured in medium conditioned by an established teratocarcinoma stem cell line. This suggests that such conditioned medium might contain a growth factor that stimulates the proliferation or inhibits the differentiation of normal pluripotent embryonic cells, or both. This method of obtaining embryonic stem cells makes feasible the isolation of pluripotent cells lines from various types of noninbred embryo, including those carrying mutant genes. The availability of such cell lines should made possible new approaches to the study of early mammalian development. more

Topics: Embryonic stem cell (71%), Stem cell (69%), Embryoid body (68%) more

5,271 Citations

No. of papers in the topic in previous years

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Topic's top 5 most impactful authors

George Q. Daley

131 papers, 25.7K citations

Hossein Baharvand

129 papers, 4.4K citations

Austin Smith

100 papers, 30.3K citations

Christine L. Mummery

90 papers, 8.1K citations

Hans R. Schöler

89 papers, 12.9K citations

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