Showing papers on "Energy source published in 1999"

Ketone bodies: a review of physiology, pathophysiology and application of monitoring to diabetes

Abstract: Ketone bodies are produced by the liver and used peripherally as an energy source when glucose is not readily available. The two main ketone bodies are acetoacetate (AcAc) and 3-beta-hydroxybutyrate (3HB), while acetone is the third, and least abundant, ketone body. Ketones are always present in the blood and their levels increase during fasting and prolonged exercise. They are also found in the blood of neonates and pregnant women. Diabetes is the most common pathological cause of elevated blood ketones. In diabetic ketoacidosis (DKA), high levels of ketones are produced in response to low insulin levels and high levels of counterregulatory hormones. In acute DKA, the ketone body ratio (3HB:AcAc) rises from normal (1:1) to as high as 10:1. In response to insulin therapy, 3HB levels commonly decrease long before AcAc levels. The frequently employed nitroprusside test only detects AcAc in blood and urine. This test is inconvenient, does not assess the best indicator of ketone body levels (3HB), provides only a semiquantitative assessment of ketone levels and is associated with false-positive results. Recently, inexpensive quantitative tests of 3HB levels have become available for use with small blood samples (5-25 microl). These tests offer new options for monitoring and treating diabetes and other states characterized by the abnormal metabolism of ketone bodies.

Seal & cut electrosurgical instrument

Abstract: An electrosurgical instrument has a handle and a body which position and close a jaw about a tissue site for simultaneously cutting and sealing relatively large tissue structures. The jaw includes an electrosurgical cutting member, which may be a blade or wire, against which tissue is biased along a cut line, and a clamping assembly that clamps a region adjacent to or surrounding the cut line. The clamping assembly includes sealing electrodes for heating the region and welding tissue along the side of the cut as the cutter parts the tissue. The clamping assembly preferably has first and second clamping jaws extending in parallel to grip the tissue as tension is released by the cut, allowing dependable and complete sealing of the cut ends over an extended time while the tissue is immobilized. Sealing electrodes formed of thermally conductive material may be covered by a thinner coating or thermally non-impeding or heat transfer cover, which preferably includes a material to assure biocompatibility and prevent sticking. The handle, body and jaw may be configured in the shape of a hemostat to allow simple positioning, or may be configured with an elongated body and a mechanism that operates the jaw through a stationary or telescoping handle for endosurgical use through a small incision. The blade may extend entirely to the tip, or the clamp assembly may extend forward of the blade. An energy ratio input element may attach to an energy source to apportion electrosurgical energy between the cutting member and the sealing electrodes. The element may include a pair of transformers, or a transformer with a split secondary or a tapped secondary winding, with the primary configured for connection to the output of an RF scalpel drive console. The windings of the transformer match impedances and power requirements of the different cutting and sealing electrodes to coordinate effective tissue sealing and welding with the cutting operation.

Bipolar surgical instruments having focused electrical fields

Abstract: A bipolar surgical device comprises a pair of actuable jaws. A first electrode member which optionally includes a line of electrically coupled tissue-penetrating elements is formed on one of the jaws, and a second electrode member which optionally includes a line of electrically coupled tissue-penetrating elements is formed on the same or the other jaw. The electrode members are laterally spaced-apart and arranged in a parallel, usually linear manner so that the lateral distance therebetween remains generally constant. In operation, tissue may be grasped between the jaws so that the electrode members contact and/or the tissue-penetrating elements enter into the tissue. By energizing the electrode members at opposite polarities using a high frequency energy source, tissue between the jaws will be heated, coagulated, and/or necrosed, while heating of tissue outside of the lines will be minimized.

A nanomechanical device based on the B–Z transition of DNA

Abstract: The assembly of synthetic, controllable molecular mechanical systems1,2,3,4,5,6,7 is one of the goals of nanotechnology. Protein-based molecular machines, often driven by an energy source such as ATP, are abundant in biology8,9. It has been shown previously that branched motifs of DNA can provide components for the assembly of nanoscale objects10, links11 and arrays12. Here we show that such structures can also provide the basis for dynamic assemblies: switchable molecular machines. We have constructed a supramolecular device consisting of two rigid DNA ‘double-crossover’ (DX) molecules connected by 4.5 double-helical turns. One domain of each DX molecule is attached to the connecting helix. To effect switchable motion in this assembly, we use the transition between the B and Z13,14 forms of DNA. In conditions that favour B-DNA, the two unconnected domains of the DX molecules lie on the same side of the central helix. In Z-DNA-promoting conditions, however, these domains switch to opposite sides of the helix. This relative repositioning is detected by means of fluorescence resonance energy transfer spectroscopy, which measures the relative proximity of two dye molecules attached to the free ends of the DX molecules. The switching event induces atomic displacements of 20–60 A.

Function and Regulation of Yeast Hexose Transporters

Abstract: Glucose, the most abundant monosaccharide in nature, is the principal carbon and energy source for nearly all cells. The first, and rate-limiting, step of glucose metabolism is its transport across the plasma membrane. In cells of many organisms glucose ensures its own efficient metabolism by serving as an environmental stimulus that regulates the quantity, types, and activity of glucose transporters, both at the transcriptional and posttranslational levels. This is most apparent in the baker’s yeast Saccharomyces cerevisiae, which has 20 genes encoding known or likely glucose transporters, each of which is known or likely to have a different affinity for glucose. The expression and function of most of these HXT genes is regulated by different levels of glucose. This review focuses on the mechanisms S. cerevisiae and a few other fungal species utilize for sensing the level of glucose and transmitting this information to the nucleus to alter HXT gene expression. One mechanism represses transcription of some HXT genes when glucose levels are high and works through the Mig1 transcriptional repressor, whose function is regulated by the Snf1-Snf4 protein kinase and Reg1-Glc7 protein phosphatase. Another pathway induces HXT expression in response to glucose and employs the Rgt1 transcriptional repressor, a ubiquitin ligase protein complex (SCFGrr1) that regulates Rgt1 function, and two glucose sensors in the membrane (Snf3 and Rgt2) that bind glucose and generate the intracellular signal to which Rgt1 responds. These two regulatory pathways collaborate with other, less well-understood, pathways to ensure that yeast cells express the glucose transporters best suited for the amount of glucose available.

Fatty acids, lipotoxicity and insulin secretion.

Abstract: tant energy source for most body tissues, particularly during periods of food deprivation, but recent evidence suggests that these same molecules subserve a much broader function in whole body fuel homeostasis by virtue of their ability to act as potent signalling entities in a variety of cellular processes. One such auxiliary role of NEFA is to heighten the responsiveness of the pancreatic beta cell to a variety of insulin secretagogues. Importantly, this fatty acid-beta cell interaction, though designed by nature for physiological purposes, can, under certain circumstances, take on a pathophysiological dimension. Some new developments surrounding this Jekyll and Hyde character of fatty acids will be reviewed briefly below.

Regulation of glucose production by the liver.

Abstract: Glucose is an essential nutrient for the human body. It is the major energy source for many cells, which depend on the bloodstream for a steady supply. Blood glucose levels, therefore, are carefully maintained. The liver plays a central role in this process by balancing the uptake and storage of glucose via glycogenesis and the release of glucose via glycogenolysis and gluconeogenesis. The several substrate cycles in the major metabolic pathways of the liver play key roles in the regulation of glucose production. In this review, we focus on the short- and long-term regulation glucose-6-phosphatase and its substrate cycle counter-part, glucokinase. The substrate cycle enzyme glucose-6-phosphatase catalyzes the terminal step in both the gluconeogenic and glycogenolytic pathways and is opposed by the glycolytic enzyme glucokinase. In addition, we include the regulation of GLUT 2, which facilitates the final step in the transport of glucose out of the liver and into the bloodstream.

A molecular sensor that allows a gut commensal to control its nutrient foundation in a competitive ecosystem

Abstract: Little is known about how members of the indigenous microflora interact with their mammalian hosts to establish mutually beneficial relationships. We have used a gnotobiotic mouse model to show that Bacteroides thetaiotaomicron, a component of the intestinal microflora of mice and humans, uses a repressor, FucR, as a molecular sensor of L-fucose availability. FucR coordinates expression of an operon encoding enzymes in the L-fucose metabolic pathway with expression of another locus that regulates production of fucosylated glycans in intestinal enterocytes. Genetic and biochemical studies indicate that FucR does this by using fucose as an inducer at one locus and as a corepressor at the other locus. Coordinating this commensal's immediate nutritional requirements with production of a host-derived energy source is consistent with its need to enter and persist within a competitive ecosystem.

Optical Spectroscopy of the IRAS 1 Jy Sample of Ultraluminous Infrared Galaxies

Abstract: This paper discusses the optical spectroscopic properties of the IRAS 1 Jy sample (f60 > 1 Jy) of ultraluminous infrared galaxies (ULIGs: LIR > 1012 L☉; H0 = 75 km s-1 Mpc-1 and q0 = 0). One hundred eight of the 118 1 Jy ULIGs have been observed at Δλ = 8.3 A resolution over the wavelength range ~4500-8900 A. These data are combined with large, previously published sets of optical spectroscopic data of lower luminosity infrared galaxies to look for systematic trends with infrared luminosity over the luminosity range LIR ≈ 1010.5-1013 L☉. As found in previous studies, the fraction of Seyfert galaxies among luminous infrared galaxies increases abruptly above LIR ≈ 1012.3 L☉—about 50% of the galaxies with LIR > 1012.3 L☉ present Seyfert characteristics. Many of the optical and infrared spectroscopic properties of the Seyfert galaxies are consistent with the presence of a genuine active galactic nucleus (AGN). About 30% of these galaxies are Seyfert 1 galaxies with broad-line regions similar to those of optical quasars. Published near-infrared spectroscopy also suggests that many of the Seyfert 2 galaxies (especially those with warm IRAS 25-60 μm colors) are in fact obscured Seyfert 1 galaxies with broad (2000 km s-1) recombination lines at 2 μm, where dust obscuration is less important. The percentage of Seyfert 1 ULIGs increases with infrared luminosity, contrary to the predictions of the standard unification model for Seyfert galaxies. Comparisons of the broad-line luminosities of optical and obscured Seyfert 1 ULIGs with those of optically selected quasars of comparable bolometric luminosity suggest that the dominant energy source in most of these ULIGs is the same as in optical quasars, namely mass accretion onto a supermassive black hole, rather than a starburst. These results are consistent with recently published ISO, ASCA, and VLBI data. On the other hand, there is no unambiguous optical or near-infrared spectroscopic evidence for AGNs in ULIGs optically classified as H II region galaxies (~30% of the whole sample) or as LINERs (~40%). The apparent lack of energetically important AGNs in these objects supports the results from recent mid-infrared spectroscopy with ISO. Photoionization by hot stars from recent starbursts appears to be the dominant source of ionization in the objects with H II region-like spectra, while both hot stars and shocks may contribute to the ionization in ULIGs with LINER-like spectra. The weaker Hβ and Mg I b stellar absorption features, larger Hα emission equivalent widths, and bluer optical continuum colors in objects with higher infrared luminosities suggest that the starbursts took place more recently (few times 107 yr) and/or are more important (~10% of the galaxy mass) in ULIGs than in their lower luminosity counterparts. As found in optically selected starbursts, the emission-line gas in ULIGs is dustier than the stellar populations that are producing the optical continuum. The color excess derived from the Balmer-line ratio does not significantly depend on the infrared luminosity, optical spectral type, or IRAS 25-60 μm color of the luminous infrared galaxies. These results suggest that the optical method used to determine the color excess in infrared galaxies underestimates the amount of dust in the dustier objects.

What is river health

Abstract: Summary 1. Traditionally the assessment of river water quality has been based solely on the measurement of physical, chemical and some biological characteristics. While these measurements may be efficient for regulating effluent discharges and protecting humans, they are not very useful for large-scale management of catchments or for assessing whether river ecosystems are being protected. 2. Measurements of aquatic biota, to identify structural or functional integrity of ecosystems, have recently gained acceptance for river assessment. Empirical evidence from studies of river ecosystems under stress suggests that a small group of biological ecosystem-level indicators can assess river condition. However, physical and chemical features of the environment affect these indicators, the structure and function of which may be changed by human activities. 3. The term ‘river health’, applied to the assessment of river condition, is often seen as being analogous with human health, giving many a sense of understanding. Unfortunately, the meaning of ‘river health’ remains obscure. It is not clear what aspects of river health sets of ecosystem-level indicators actually identify, nor how physical, chemical and biological characteristics may be integrated into measures rather than just observations of cause and effect. 4. Increased examination of relationships between environmental variables that affect aquatic biota, such as habitat structure, flow regime, energy sources, water quality and biotic interactions and biological condition, are required in the study of river health.

The Canada-United Kingdom Deep Submillimeter Survey. II. First Identifications, Redshifts, and Implications for Galaxy Evolution*

Abstract: Identifications are sought for 12 submillimeter sources detected in a deep submillimeter survey. Six are securely identified, two have probable identifications, and four remain unidentified with IAB > 25. Spectroscopic and estimated photometric redshifts indicate that four of the sources have z 3. The spectral energy distributions of the identifications, as defined by measurements or upper limits to the flux densities at 8000 A, at 15, 450, 850 μm, and at 6 cm, are consistent with the spectral energy distributions of high-extinction starbursts such as Arp 220. The far-IR luminosities of the sources at z > 0.5 are of order 3 × 1012 h-250 L☉, i.e., slightly larger than that of Arp 220. As with local ultraluminous infrared galaxies, the optical luminosities of the identified galaxies are comparable to present-day L*, and the optical morphologies of many of the galaxies show evidence for mergers or highly disruptive interactions. Based on this small sample, the cumulative bolometric luminosity function shows strong evolution to z~1, but weaker or possibly even negative evolution beyond. The redshift dependence of the far-IR luminosity density does not appear, at this early stage, to be inconsistent with that seen in the ultraviolet luminosity density. Although the computation of bolometric luminosities is quite uncertain, the population of very luminous galaxies that is detected in the surveys at z > 1 is already matching, in the far-IR, the bolometric output in the ultraviolet of the whole optically selected population. Assuming that the energy source in the far-IR is massive stars, this suggests that the total luminous output from star formation in the universe will be dominated by the far-IR emission once the lower luminosity sources, below the current far-IR detection threshold, are included. Furthermore, the detected systems have individual star formation rates (exceeding 300 h-250 M☉ yr-1) that are much higher than seen in the ultraviolet-selected samples and that are sufficient to form substantial stellar populations on dynamical timescales of 108 yr. The association with mergerlike morphologies and the obvious presence of dust makes it attractive to identify these systems as forming the metal-rich spheroid population, in which case we would infer that much of this activity has occurred relatively recently, at z~2.

Nitrogen management and the future of food : lessons from the management of energy and carbon

Abstract: The food system dominates anthropogenic disruption of the nitrogen cycle by generating excess fixed nitrogen. Excess fixed nitrogen, in various guises, augments the greenhouse effect, diminishes stratospheric ozone, promotes smog, contaminates drinking water, acidifies rain, eutrophies bays and estuaries, and stresses ecosystems. Yet, to date, regulatory efforts to limit these disruptions largely ignore the food system. There are many parallels between food and energy. Food is to nitrogen as energy is to carbon. Nitrogen fertilizer is analogous to fossil fuel. Organic agriculture and agricultural biotechnology play roles analogous to renewable energy and nuclear power in political discourse. Nutrition research resembles energy end-use analysis. Meat is the electricity of food. As the agriculture and food system evolves to contain its impacts on the nitrogen cycle, several lessons can be extracted from energy and carbon: (i) set the goal of ecosystem stabilization; (ii) search the entire production and consumption system (grain, livestock, food distribution, and diet) for opportunities to improve efficiency; (iii) implement cap-and-trade systems for fixed nitrogen; (iv) expand research at the intersection of agriculture and ecology, and (v) focus on the food choices of the prosperous. There are important nitrogen-carbon links. The global increase in fixed nitrogen may be fertilizing the Earth, transferring significant amounts of carbon from the atmosphere to the biosphere, and mitigating global warming. A modern biofuels industry someday may produce biofuels from crop residues or dedicated energy crops, reducing the rate of fossil fuel use, while losses of nitrogen and other nutrients are minimized.

Tomato seed and root exudate sugars: composition, utilization by Pseudomonas biocontrol strains and role in rhizosphere colonization

Abstract: The role of tomato seed and root exudate sugars as nutrients for Pseudomonas biocontrol bacteria was studied. To this end, the major exudate sugars of tomato seeds, seedlings and roots were identified and quantified using high-performance liquid chromatographic (HPLC) analysis. Glucose, fructose and maltose were present in all studied growth stages of the plant, but the ratios of these sugars were strongly dependent on the developmental stage. In order to study the putative role of exudate sugar utilization in rhizosphere colonization, two approaches were adopted. First, after co-inoculation on germinated tomato seeds, the root-colonizing ability of the efficient root-colonizing P. fluorescens strain WCS365 in a gnotobiotic quartz sand-plant nutrient solution system was compared with that of other Pseudomonas biocontrol strains. No correlation was observed between the colonizing ability of a strain and its ability to use the major exudate sugars as the only carbon and energy source. Secondly, a Tn5lacZ mutant of P. fluorescens strain WCS365, strain PCL1083, was isolated, which is impaired in its ability to grow on simple sugars, including those found in exudate. The mutation appeared to reside in zwf, which encodes glucose-6-phosphate dehydrogenase. The mutant grows as well as the parental strain on other media, including tomato root exudate. After inoculation of germinated sterile tomato seeds, the mutant cells reached the same population levels at the root tip as the wild-type strain, both alone and in competition, indicating that the ability to use exudate sugars does not play a major role in tomato root colonization, despite the fact that sugars have often been reported to represent the major exudate carbon source. This conclusion is supported by the observation that the growth of mutant PCL1083 in vitro is inhibited by glucose, a major exudate sugar, at a concentration of 0.001%, which indicates that the glucose concentration in the tomato rhizosphere is very low.

The Canada-UK Deep Sub-Millimeter Survey II: First identifications, redshifts and implications for galaxy evolution

Abstract: Identifications are sought for 12 sub-mm sources detected by Eales et al (1998). Six are securely identified, two have probable identifications and four remain unidentified with I_AB > 25. Spectroscopic and estimated photometric redshifts indicate that four of the sources have z 3. The spectral energy distributions of the identifications are consistent with those of high extinction starbursts such as Arp 220. The far-IR luminosities of the sources at z > 0.5 are of order 3 x 10^12 h_50^-2 L_sun, i.e. slightly larger than that of Arp 220. Based on this small sample, the cumulative bolometric luminosity function shows strong evolution to z ~ 1, but weaker or possibly even negative evolution beyond. The redshift dependence of the far-IR luminosity density does not appear, at this early stage, to be inconsistent with that seen in the ultraviolet luminosity density. Assuming that the energy source in the far-IR is massive stars, the total luminous output from star-formation in the Universe is probably dominated by the far-IR emission. The detected systems have individual star-formation rates (exceeding 300 h_50^-2 M_O yr^-1) that are much higher than seen in the ultraviolet selected samples, and which are sufficient to form substantial stellar populations on dynamical timescales of 10^8 yr. The association with merger-like morphologies and the obvious presence of dust makes it attractive to identify these systems as forming the metal-rich spheroid population, in which case we would infer that much of this activity has occurred relatively recently, at z ~ 2.

Construction of Environmental DNA Libraries in Escherichia coli and Screening for the Presence of Genes Conferring Utilization of 4-Hydroxybutyrate

Abstract: Environmental DNA libraries from three different soil samples were constructed. The average insert size was 5 to 8 kb and the percentage of plasmids with inserts was approximately 80%. The recombinant Escherichia coli strains (approximately 930,000) were screened for 4-hydroxybutyrate utilization. Thirty-six positive E. coli clones were obtained during the initial screen, and five of them contained a recombinant plasmid (pAH1 to pAH5) which conferred a stable 4-hydroxybutyrate-positive phenotype. These E. coli clones were studied further. All five were able to grow with 4-hydroxybutyrate as sole carbon and energy source and exhibited 4-hydroxybutyrate dehydrogenase activity in crude extracts. Sequencing of pAH5 revealed a gene homologous to the gbd gene of Ralstonia eutropha, which encodes a 4-hydroxybutyrate dehydrogenase. Two other genes (orf1 and orf6) conferring utilization of 4-hydroxybutyrate were identified during subcloning and sequencing of the inserts of pAH1 and pAH3. The deduced orf1 gene product showed similarities to members of the DedA family of proteins. The sequence of the deduced orf6 gene product harbors the fingerprint pattern of enoyl-coenzyme A hydratases/isomerases. The other sequenced inserts of the plasmids recovered from the positive clones revealed no significant similarity to any other gene or gene product whose sequence is available in the National Center for Biotechnology Information databases.

Polycyclic aromatic hydrocarbon degradation by a new marine bacterium, Neptunomonas naphthovorans gen. nov., sp. nov

Abstract: Two strains of bacteria were isolated from creosote-contaminated Puget Sound sediment based on their ability to utilize naphthalene as a sole carbon and energy source. When incubated with a polycyclic aromatic hydrocarbon (PAH) compound in artificial seawater, each strain also degraded 2-methylnaphthalene and 1-methylnaphthalene; in addition, one strain, NAG-2N-113, degraded 2,6-dimethylnaphthalene and phenanthrene. Acenaphthene was not degraded when it was used as a sole carbon source but was degraded by both strains when it was incubated with a mixture of seven other PAHs. Degenerate primers and the PCR were used to isolate a portion of a naphthalene dioxygenase iron-sulfur protein (ISP) gene from each of the strains. A phylogenetic analysis of PAH dioxygenase ISP deduced amino acid sequences showed that the genes isolated in this study were distantly related to the genes encoding naphthalene dioxygenases of Pseudomonas and Burkholderia strains. Despite the differences in PAH degradation phenotype between the new strains, the dioxygenase ISP deduced amino acid fragments of these organisms were 97.6% identical. 16S ribosomal DNA-based phylogenetic analysis placed these bacteria in the gamma-3 subgroup of the Proteobacteria, most closely related to members of the genus Oceanospirillum. However, morphologic, physiologic, and genotypic differences between the new strains and the oceanospirilla justify the creation of a novel genus and species, Neptunomonas naphthovorans. The type strain of N. naphthovorans is strain NAG-2N-126.

Prolonging cell‐free protein synthesis with a novel ATP regeneration system

Abstract: A new approach for the regeneration of adenosine triphosphate (ATP) during cell-free protein synthesis was developed to prolong the synthesis and also to avoid the accumulation of inorganic phosphate. This approach was demonstrated in a batch system derived from Escherichia coli. Contrary to the conventional methods in which exogenous energy sources contain high-energy phosphate bonds, the new system was designed to generate continuously the required high-energy phosphate bonds within the reaction mixture, thereby recycling the phosphate released during protein synthesis. If allowed to accumulate, phosphate inhibits protein synthesis, most likely by reducing the concentration of free magnesium ion. Pediococcus sp. pyruvate oxidase, when introduced in the reaction mixture along with thiamine pyrophosphate (TPP) and flavin adenine dinucleotide (FAD), catalyzed the generation of acetyl phosphate from pyruvate and inorganic phosphate. Acetyl kinase, already present with sufficient activity in Escherichia coli S30 extract, then catalyzed the regeneration of ATP. Oxygen is required for the generation of acetyl phosphate and the H(2)O(2) produced as a byproduct is sufficiently degraded by endogenous catalase activity. Through the continuous supply of chemical energy, and also through the prevention of inorganic phosphate accumulation, the duration of protein synthesis is extended up to 2 h. Protein accumulation levels also increase. The synthesis of human lymphotoxin receives greater benefit than than that of chloramphenicol acetyl transferase, because the former is more sensitive to phosphate inhibition. Finally, through repeated addition of pyruvate and amino acids during the reaction period, protein synthesis continued for 6 h in the new system, resulting in a final yield of 0.7 mg/mL.

Low temperature silicon wafer bond process with bulk material bond strength

Abstract: The present invention includes a method for bonding one semiconductor surface to a second semiconductor surface. The method includes providing a first article that has a semiconductor surface and a second article that has a semiconductor surface. The semiconductor surfaces are annealed with an energy source wherein energy is confined to the semiconductor surfaces. The annealed surfaces are bonded to each other.

Mixing and Energetics of the Oceanic Thermohaline Circulation

Abstract: Using an idealized tube model and scaling analysis, the physics supporting the oceanic thermohaline circulation is examined. Thermal circulation in the tube model can be classified into two categories. When the cooling source is at a level higher than that of the heating source, the thermal circulation is friction-controlled; thus, mixing is not important in determining the circulation rate. When the cooling source is at a level lower than that of the heating source, the circulation is mixing controlled; thus, weak (strong) mixing will lead to weak (strong) thermal circulation. Within realistic parameter regimes the thermohaline circulation requires external sources of mechanical energy to support mixing in order to maintain the basic stratification. Thus, the oceanic circulation is only a heat conveyor belt, not a heat engine. Simple scaling shows that the meridional mass and heat fluxes are linearly proportional to the energy supplied to mixing. The rate of tidal dissipation in the open oceans (excluding the shallow marginal seas) is about 0.9‐1.3 (31012 W); the rate of potential energy generated by geothermal heating is estimated to be 0.5 3 1012 W. Accordingly, the global-mean rate of mixing inferred from oceanic climatological data is about 0.22 3 1024 m2 s21. Using a primitive equation model, numerical experiments based on a fixed energy source for mixing have been carried out in order to test the scaling law. In comparison with models under fixed rate of mixing, a model under a fixed energy for mixing is less sensitive to changes in the forcing conditions due to climatic changes. Under a surface relaxation condition for temperature and standard parameters, the model is well within the region of Hopf bifurcation, so decadal variability is expected.

Micro thermoelectric modules and their application to wristwatches as an energy source

Abstract: Microthermoelectric (TE) coolers are currently used in high power electronic devices such as laser diodes to stabilize temperature. With the aim of miniaturizing this technology, we have developed a fabrication technique to create micro-TE modules with a cross-section element size of about 100 /spl mu/m/spl times/100 /spl mu/m and a height of several hundred mm. We have previously reported the cooling properties of a module fabricated by this technique. With the recent advances in micro-electric technology, which has decreased the energy consumption in an electric wristwatch to about 1 /spl mu/W, there has been increased discussion about utilizing these micro-TE modules to generate energy of several /spl mu/W to power a wristwatch. In response, we have developed a micro-TE module with an overall size of 2 /spl times/ 2 /spl times/ 1.3 mm consisting of more than 50 pairs of elements, and have succeeded in making and marketing a TE powered wristwatch. This paper presents the fabrication and the properties of the micro-TE module, as well as the TE powered wristwatch.

Triglyceride content of bovine oocytes and early embryos

Abstract: A microfluorescence technique was used to measure the triglyceride content of a minimum of two bovine oocytes or preimplantation embryos up to the hatched blastocyst stage. Embryos were produced in vitro from abattoir-derived ovaries and grown in medium containing synthetic oviductal fluid, amino acids and BSA (SOFaaBSA medium); 10% fetal calf serum was added to some of the embryos at the four-cell stage. Before maturation, the triglyceride content of oocytes was 59 +/- 1.37 ng and it decreased (P < 0.05) after maturation to 46 +/- 0.85 ng. A decrease in triglyceride content (P < 0.05) was also observed after fertilization with the formation of the two-cell embryo (34 +/- 1.80 ng). In the absence of serum, the triglyceride content remained relatively constant from the two-cell to the hatched blastocyst stage. The triglyceride content of blastocysts produced in vivo was similar (33 +/- 0.70 ng) to that of blastocysts produced in vitro in the absence of serum. In contrast, the triglyceride content of embryos grown with 10% fetal calf serum increased steadily from the 9-16-cell stage to a value in hatched blastocysts (62 +/- 1.14 ng) almost double that in serum-free conditions. These results indicate that triglyceride may act as energy source during bovine oocyte maturation and fertilization and that the presence of serum causes excessive synthesis or accumulation of triglyceride in early embryos.

De novo lipogenesis in humans: metabolic and regulatory aspects

Abstract: The enzymatic pathway for converting dietary carbohydrate (CHO) into fat, or de novo lipogenesis (DNL), is present in humans, whereas the capacity to convert fats into CHO does not exist. Here, the quantitative importance of DNL in humans is reviewed, focusing on the response to increased intake of dietary CHO. Eucaloric replacement of dietary fat by CHO does not induce hepatic DNL to any substantial degree. Similarly, addition of CHO to a mixed diet does not increase hepatic DNL to quantitatively important levels, as long as CHO energy intake remains less than total energy expenditure (TEE). Instead, dietary CHO replaces fat in the whole-body fuel mixture, even in the post-absorptive state. Body fat is thereby accrued, but the pathway of DNL is not traversed; instead, a coordinated set of metabolic adaptations, including resistance of hepatic glucose production to suppression by insulin, occurs that allows CHO oxidation to increase and match CHO intake. Only when CHO energy intake exceeds TEE does DNL in liver or adipose tissue contribute significantly to the whole-body energy economy. It is concluded that DNL is not the pathway of first resort for added dietary CHO, in humans. Under most dietary conditions, the two major macronutrient energy sources (CHO and fat) are therefore not interconvertible currencies; CHO and fat have independent, though interacting, economies and independent regulation. The metabolic mechanisms and physiologic implications of the functional block between CHO and fat in humans are discussed, but require further investigation.

Thermal securing anastomosis systems

Abstract: This invention is a non-suture anastomosis system for securing a bypass graft (16) to a host vessel (38) or other tubular structure including a bypass graft (16), and a fitting (60). A compression mechanism may be used with the system for attachment of the bypass graft (16) to the fitting (60). An electrode (44) is connected to the fitting (60), and an energy source. The energy source transmits energy to the electrode which causes the adjacent tissue to rise in temperature, and bond to a vessel or fitting (60).

Biodegradation of Methyl tert-Butyl Ether by a Bacterial Pure Culture

Abstract: A bacterial strain, PM1, which is able to utilize methyl tert-butyl ether (MTBE) as its sole carbon and energy source, was isolated from a mixed microbial consortium in a compost biofilter capable of degrading MTBE. Initial linear rates of MTBE degradation by 2 x 10(6) cells ml(-1) were 0.07, 1.17, and 3.56 microg ml(-1) h(-1) for initial concentrations of 5, 50, and 500 microg MTBE ml(-1), respectively. When incubated with 20 microg of uniformly labeled [(14)C]MTBE ml(-1), strain PM1 converted 46% to (14)CO(2) and 19% to (14)C-labeled cells within 120 h. This yield is consistent with the measurement of protein accumulation at different MTBE concentrations from which was estimated a biomass yield of 0.18 mg of cells mg MTBE(-1). Strain PM1 was inoculated into sediment core material collected from a contaminated groundwater plume at Port Hueneme, California, in which there was no evidence of MTBE degradation. Strain PM1 readily degraded 20 microg of MTBE ml(-1) added to the core material. The rate of MTBE removal increased with additional inputs of 20 microg of MTBE ml(-1). These results suggest that PM1 has potential for use in the remediation of MTBE-contaminated environments.

Differential Rotation and Turbulence in Extended H I Disks

Abstract: When present, extended disks of neutral hydrogen around spiral galaxies show a remarkably uniform velocity dispersion of ~6 km s-1. Since stellar winds and supernovae are largely absent in such regions, neither the magnitude nor the constancy of this number can be accounted for in the classical picture in which interstellar turbulence is driven by stellar energy sources. Here we suggest that magnetic fields with strengths of a few microgauss in these extended disks allow energy to be extracted from galactic differential rotation through MHD-driven turbulence. The magnitude and constancy of the observed velocity dispersion may be understood if its value is Alfvenic. Moreover, by providing a simple explanation for a lower bound to the gaseous velocity fluctuations, MHD processes may account for the sharp outer edge to star formation in galaxy disks.