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Enzyme assay

About: Enzyme assay is a research topic. Over the lifetime, 25406 publications have been published within this topic receiving 690706 citations.


Papers
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Journal ArticleDOI
TL;DR: A photometric method for determining acetylcholinesterase activity of tissue extracts, homogenates, cell suspensions, etc., has been described and Kinetic constants determined by this system for erythrocyte eholinesterases are presented.

23,015 citations

Journal ArticleDOI
TL;DR: A sensitive, fixed-time, spectrophotometric assay for angiotensin-converting enzyme measures the rate of production of hippuric acid from hippuryl-L -histidyl- L -leucine (HHL).

2,493 citations

Book
01 Jan 1979
TL;DR: Basic Principles of Chemical Kinetics Introduction to Enzyme Kinetics "Alternative" Enzymes Practical Aspects of Kinetics Deriving Steady-state Rate Equations Reversible Inhibition and Activation Tight-binding and Irreversible Inhibitors
Abstract: Basic Principles of Chemical Kinetics Introduction to Enzyme Kinetics "Alternative" Enzymes Practical Aspects of Kinetics Deriving Steady-state Rate Equations Reversible Inhibition and Activation Tight-binding and Irreversible Inhibitors Reactions of More than One Substrate Use of Isotopes for Studying Enzyme Mechanisms Effect of pH on Enzyme Activity Temperature Effects on Enzyme Activity Regulation of Enzyme Activity Multienzyme Systems Fast Reactions Estimation of Kinetic Constants Standards for Reporting Enzymology Data Solutions and Notes to Problems Index

2,220 citations

Journal ArticleDOI
02 Dec 1999-Nature
TL;DR: A membrane-bound enzyme activity that cleaves full-length APP at the β-secretase cleavage site is described and found to be the predominant β-cleavage activity in human brain, and it is found that human brain β- secretase is a new membrane- bound aspartic proteinase.
Abstract: Proteolytic processing of the amyloid precursor protein (APP) generates amyloid β (Aβ) peptide, which is thought to be causal for the pathology and subsequent cognitive decline in Alzheimer's disease Cleavage by β-secretase at the amino terminus of the Aβ peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of β-cleaved soluble APP1, and a corresponding cell-associated carboxy-terminal fragment Cleavage of the C-terminal fragment by γ-secretase(s) leads to the formation of Aβ The pathogenic mutation K670M671 → N670L671 at the β-secretase cleavage site in APP2, which was discovered in a Swedish family with familial Alzheimer's disease, leads to increased β-secretase cleavage of the mutant substrate3 Here we describe a membrane-bound enzyme activity that cleaves full-length APP at the β-secretase cleavage site, and find it to be the predominant β-cleavage activity in human brain We have purified this enzyme activity to homogeneity from human brain using a new substrate analogue inhibitor of the enzyme activity, and show that the purified enzyme has all the properties predicted for β-secretase Cloning and expression of the enzyme reveals that human brain β-secretase is a new membrane-bound aspartic proteinase

1,777 citations

Journal ArticleDOI
TL;DR: The spectrophotofluorometric determination of hydroxylated benzo[a]pyrene products is sufficiently sensitive to detect 10-12 mole per ml and has great utility in measuring the hydroxymatic activity of cells grown in culture.

1,664 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023118
2022232
2021239
2020330
2019392
2018415