Topic
Esterase
About: Esterase is a research topic. Over the lifetime, 7622 publications have been published within this topic receiving 168270 citations.
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TL;DR: Photographs and densitometer tracings of gels showing changes during germination and growth of wheat indicate changes in soluble proteins and the isoenzyme bands of the following enzymes.
72 citations
TL;DR: Among microalgae species, R. salina exhibited the highest sensitivity, and the inhibitor effect was higher for the C(13) LAS homologue than for C(11), in both responses analyzed.
72 citations
TL;DR: Enzyme inhibition studies and analysis of substitution mutants of the carboxylesterase revealed the typical catalytic mechanism of a serine hydrolase with a catalytic triad composed of serine, glutamic acid, and histidine.
Abstract: We have identified a carboxylesterase produced in liquid cultures of the thermophilic actinomycete Thermobifida fusca KW3 that were supplemented with poly(ethylene terephthalate) fibers. The enzyme hydrolyzed highly hydrophobic, synthetic cyclic poly(ethylene terephthalate) trimers with an optimal activity at 60°C and a pH of 6. V
max and K
m values for the hydrolysis were 9.3 µmol−1 min−1 mg−1 and 0.5 mM, respectively. The esterase showed high specificity towards short and middle chain-length fatty acyl esters of p-nitrophenol. The enzyme retained 37% of its activity after 96 h of incubation at 50°C and a pH of 8. Enzyme inhibition studies and analysis of substitution mutants of the carboxylesterase revealed the typical catalytic mechanism of a serine hydrolase with a catalytic triad composed of serine, glutamic acid, and histidine.
71 citations
TL;DR: Acetylcholinesterase and pseudocholinesTERase activities were demonstrated in adult Schistosoma haematobium, S. mansoni, and S. rodhaini with wide-spectrum substrates associated with specific inhibitors with results confirmed by using specific substrates.
71 citations
TL;DR: Two polyester polyurethane (PU)-degrading enzymes from Pseudomonaschlororaphis were purified to electrophoretic homogeneity as indicated by sodium dodecyl-polyacrylamide gelelectrophoresis (SDS–PAGE) indicating both are serine-hydrolases.
71 citations