Topic
Esterase
About: Esterase is a research topic. Over the lifetime, 7622 publications have been published within this topic receiving 168270 citations.
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TL;DR: A new esterolytic enzyme identified by screening a metagenomic sample collected from a hot spring in Kamchatka, Russia, which is likely an Acetothermia enzyme that belongs to a new family of bacterial esterases, for which the index XV is proposed.
Abstract: Biocatalysts exerting activity against ester bonds have a broad range of applications in modern biotechnology. Here, we have identified a new esterolytic enzyme by screening a metagenomic sample collected from a hot spring in Kamchatka, Russia. Biochemical characterization of the new esterase, termed EstDZ2, revealed that it is highly active against medium chain fatty acid esters at temperatures between 25 and 60 °C and at pH values 7–8. The new enzyme is moderately thermostable with a half-life of more than six hours at 60 °C, but exhibits exquisite stability against high concentrations of organic solvents. Phylogenetic analysis indicated that EstDZ2 is likely an Acetothermia enzyme that belongs to a new family of bacterial esterases, for which we propose the index XV. One distinctive feature of this new family, is the presence of a conserved GHSAG catalytic motif. Multiple sequence alignment, coupled with computational modelling of the three-dimensional structure of EstDZ2, revealed that the enzyme lacks the largest part of the “cap” domain, whose extended structure is characteristic for the closely related Family IV esterases. Thus, EstDZ2 appears to be distinct from known related esterolytic enzymes, both in terms of sequence characteristics, as well as in terms of three-dimensional structure.
55 citations
TL;DR: The thermal and pH stability profiles for the enzyme showed that the thermophilic bacterium A. gonensis G2 secretes an extracellular thermoalkalophilic PHB depolymerase active at 60 degrees C, and stable at this temperature for 120 min at pH 7.5 and for 24 h at pH7.5 range at 4 degrees C by retaining over 75% of its initial activities.
55 citations
TL;DR: The need to optimize various parameters when selecting a substrate for flow cytoenzymological assay or when loading other reporter fluorochromes into cells via lipophilic esters is emphasized.
55 citations
TL;DR: The results suggest that the effect of esterase 6 on the rate of sperm loss in females, which extends to 4 days after mating, is indirect since activity in the female falls rapidly after insemination.
55 citations
TL;DR: Starch-gel electrophoresis of an extract of mouse kidney disclosed that an esterase is present in mature males but absent in females and immature males, suggesting that the enzyme may be a hormone-induced one.
Abstract: Starch-gel electrophoresis of an extract of mouse kidney disclosed that an esterase is present in mature males but absent in females and immature males. When testosterone was injected for 7 days the enzyme could be detected in kidney extracts obtained from both female and young animals. Hence the enzyme may be a hormone-induced one.
55 citations