Fabiana

About: Fabiana is a research topic. Over the lifetime, 10 publications have been published within this topic receiving 190 citations.

Antimicrobial activity of selected plant species from “the Argentine Puna” against sensitive and multi-resistant bacteria

Abstract: Aim The plant species reported here are traditionally used in the “Puna” or “Altiplano” of Argentina for ailments related to bacterial infections. The aim of this study was to evaluate their antimicrobial properties against a panel of sensitive and multi-resistant Gram-positive and Gram-negative bacteria. Materials and methods The antimicrobial activity of tinctures and aqueous extracts ( Baccharis boliviensis , Chiliotrichiopsis keidelii , Chuquiraga atacamensis , Fabiana bryoides , Fabiana densa , Fabiana punensis , Frankenia triandra , Parastrephia lucida , Parastrephia lepidophylla , Parastrephia phyliciformis , Tetraglochin cristatum ) was determined using the agar macrodilution and broth microdilution methods recommended by the Clinical and Laboratory Standards Institute (CLSI, formerly NCCLS). The antibiotic resistant clinical strains were isolated from nosocomial infection in human lesions of skin and soft parts. Results The ethanolic extracts of 11 plant species inhibited the growth of one or more of the following strains: Staphylococcus aureus , Enterococcus faecalis , Escherichia coli , Klebsiella pneumoniae , Proteus mirabilis , Enterobacter cloacae , Morganella morganii , Pseudomonas aeruginosa . Ethanol extracts (tinctures) of aerial parts of Baccharis , Fabiana and Parastrephia showed the highest levels of antibacterial activity on methicillin, oxacillin and gentamicin resistant Staphylococcus with MIC values from 20 to 150 μg/ml. Baccharis boliviensis and Fabiana bryoides were more active than the other plant species on Enterococcus faecalis with different phenotype. The most interesting activity on multi-resistant Gram-negative strains was obtained from Chuquiraga atacamensis . Parastrephia species showed activity against Enterobacter cloacae , Pseudomonas aeruginosa and Proteus mirabilis . The ethanolic extracts exhibited stronger activity and broader spectrum of action than aqueous extracts. The extracts were bactericidal in most cases. Conclusions The presence of antibacterial activity in Puna plant extracts against multi-resistant bacteria give support to their traditional use for treating conditions associated with microorganisms in humans and animals and consequently seems promising for the treatment of multi-resistant bacteria.

Comparative study of antioxidant and anti-inflammatory activities and genotoxicity of alcoholic and aqueous extracts of four Fabiana species that grow in mountainous area of Argentina.

Abstract: Ethnopharmacological relevance Fabiana species (Solanaceae family) extracts have long been used in Argentinean traditional medicine as anti-inflammatories, antiseptic, bone fractures and others diseases, but there is no scientific evidence which supports their use. Aim of the study The present study was conducted to evaluate the ability of aqueous and ethanolic extracts of four Fabiana species ( Fabiana bryoides Phil., Fabiana punensis A.C. Arroyo, Fabiana densa J. Remy and Fabiana patagonica Speg.) to inhibit key enzymes in inflammatory processes, free radical scavenging properties and genotoxic effects. Materials and methods HPLC-DAD of aqueous and ethanolic extracts from four Fabiana species was established. All Fabiana extracts were evaluated on their ability to inhibit hyaluronidase and lipoxygenase enzymes to assess their activity against inflammatory mediators. Antioxidant capacity was determined using the 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assays and β-carotene-linolenic acid assay. Genotoxicity was evaluated by the Ames assay. Results The results indicated that the chromatographic patterns of four Fabiana species were different in quantity and absorption intensity of peaks. The alcoholic extract of Fabiana punensis was the most active scavenger of DPPH and ABTS + radicals (SC 50 values of 3.85 ± 0.24 and 2.56 ± 0.10 μg GAE/mL, respectively). Fabiana patagonica extracts exhibited the highest peroxyl radical scavenging activity compared with the other three taxa (IC 50 values between 1.00 ± 0.04 and 4.46 ± 0.40 μg GAE/mL for all extracts) and anti-lipoxygenase activity with IC 50 values between 12.5 and 15.5 μg GAE/mL. The absence of mutagenicity indicates that the DNA does not seem to be a relevant target for these extracts. Fabiana bryoides ethanolic extract showed an interesting effect: it inhibited spontaneous mutagenesis, which could be considered as an antimutagenic effect in the TA98 (+S9) and TA100 (+S9/−S9) strains. The potency differences found between the species could be consequence of the different phytochemical pattern observed by HPLC. Conclusions The inhibitory effects on lipoxygenase and hyaluronidase, free radical scavenging activities and lack of genotoxicity of Fabiana extracts may support the folk use of Fabiana punensis , Fabiana patagonica , Fabiana bryoides and Fabiana densa as inhibitor of inflammatory mediators.

Antibacterial diterpenoids from Fabiana densa var. ramulosa.

Abstract: A biologically monitored fractionation of the resinous exudate of Fabiana densa Remy var. ramulosa Wedd, led to the isolation of the two new diterpenes: ent-beyer-15-en-18-O-succinate and ent-beyer-15-en-18-0-oxalate as the unique compounds responsible for the observed antibacterial activity of this extract. Their structures were determined by 1 D and 2D NMR spectroscopy.

Fabianane, an unusual secoamorphane from Fabiana imbricata

Abstract: A novel seco-amorphane sesquiterene incorporating a fully saturared furo[2,3 d]1,3-dioxole system was isolated from the aerial parts of Fabiana imbricata.Such functionality is unique within the sesquiterpene family, but does have one close relative in artemisinin (the antimalarial principle from Artemisia annua), which contains a saturated pyrano[3,2-e]1,2,4-trioxin system. The structure of fabianane [1] was elucidated by nmr spectroscopy and confirmed by comparison with nmr data for artemisinin

Phylogenetic relationships of Petunia patagonica (Solanaceae) revealed by molecular and biogeographical evidence

Abstract: Petunia patagonica is restricted to the Patagonian region of Argentina and its identity is controversial. The species was described in the genus Nierembergia , and subsequently transferred to the genus Petunia . However, several morphological characteristics of P. patagonica as well as its geographical distribution differ from other Petunia species, and it has been repeatedly considered an exception in the genus. Using one nuclear and two cpDNA markers for 22 species representing seven genera of the tribe Petunieae, we analyzed phylogenetic and biogeographic evidence to clarify the phylogenetic position of P. patagonica . Our results suggest that P. patagonica is not a member of the genus Petunia and is closer to Fabiana imbricata. In addition, Calibrachoa appears basal within the Petunia , Calibrachoa , and Fabiana clades, and Fabiana and Petunia are sister genera. This result led us to reconstruct an ancestral region for this clade within the subtropical grasslands of South America. Subsequent dispersion to the Patagonian and Andean regions was inferred in the divergence of Fabiana and P. patagonica . Our work suggests a need for more studies towards a new generic placement. Ancestral area reconstruction suggests that the origin of the Calibrachoa , Fabiana and Petunia lineages was located in the subtropical grasslands of South America, and the colonization of the Andes and Patagonia seems to be divergent and was achieved only for species belonging to the Fabiana and P. patagonica clades.