Showing papers on "Fagonia cretica published in 2020"

Protective Effects of Fagonia cretica L. Extract in Cafeteria Diet Induced Obesity in Wistar Rats

Abstract: Obesity is one of the major life style disorders which may lead to undesirable effects on cholesterol, triglycerides, blood pressure and insulin resistance and eventually increases the risk of various adverse conditions like ischemic heart disease, stroke, coronary artery disease & type 2 diabetes. The present investigation was undertaken to explore protective effects of aerial parts of Fagonia cretica L. extract in cafeteria diet induced obesity in Wistar rats. Female Wistar rats were provided with cafeteria diet (CD) for the period of 10 weeks to induce obesity. Fagonia cretica L. methanolic extracts (200 & 400 mg/kg) & standard drug orlistat (30 mg/kg) were administered for last 6 weeks along with the continuation of CD. Various primary metabolic indicators of obesity like daily food consumption, body weight, lipid profile, fecal fat content & fat pads were studied. Administration of methanolic extract of Fagonia cretica L. significantly stopped increase in daily food consumption & body weight gain as compared to obese control group. Improvement in lipid profile was also observed in the all treatment groups rats as compared to obese control group rats. Obtained results validate that supplementation of Fagonia cretica L. methanolic extracts in obese rats resulted in significant protection against various indicators of obesity.

Enhancement effects of ethanolic extract of Fagonia cretica on Bisphenol A (BPA)-induced genotoxicity and biochemical changes in rats

Abstract: Fagonia cretica L. was considered to be a medical plant that was used for the treatment of different diseases, so the current study was designed to clarify whether Fagonia cretica extract (FCE) can avoid Bisphenol A (BPA)-induced genotoxicity and biochemical alterations in rats. Sixty-three male rats were used in this experiment. These animals were distributed into nine groups (seven rats each): negative control, control of corn oil, positive control that were administrated BPA in corn oil (10 mg/kg. b.w.) for 3 weeks, three protection groups received the same dose of BPA in corn oil at the same period together with FCE (3.3, 4.2 and 5.0 g/kg) daily for 3 weeks, and three therapeutic groups received FCE alone at the same doses for 10 days after cessation of BPA treatment. Genetic and biochemical studies were conducted. Genetic studies involved DNA comet assay, micronucleus test, chromosome examination, and mitotic index analysis. Biochemical studies involved liver function (AST, ALT, ALph, and Bilirubin), kidney function (urea and creatinine contents), protein profile, MDA, and endogenous antioxidative system (SOD, CAT, and ACHE) as well as nucleic acid (DNA and RNA) contents in the liver, kidney, and brain tissues. The results demonstrated that the treatment with BPA induced a significant elevation in genetic abnormalities and deleterious effects in biochemical parameters in relation to untreated control. FCE treatment was found to be significantly diminished the massive damage in the genetic constituents and dangerous alterations in biochemical parameters with respect to BPA treatment alone. These enhancements were increased by increasing the dose level of FCE. Moreover, better results were clarified by utilizing FCE as a protective agent than its utilization as a therapeutic agent especially by using the high dose (5.0 g/kg), in which mostly genetic and biochemical alterations were observed to be restored towards natural levels. These findings clarified a new insight into the protective effect of FCE in minimizing BPA-induced genotoxicity and biochemical changes in rats detecting the capability of such medicinal plant for alleviating the adverse effect of BPA.

Molecular docking and in-vitro analysis of Fagonia cretica and Berberis lyceum extract against Brucella melitensis.

Abstract: Background Brucellosis is an economically important zoonotic disease caused by the gram negative bacteria belonging to the genus Brucella. Medicinal plants are well known for a wide variety of potential antimicrobial agents that can be used as anti-microbial drugs. Method In the present study, crude ethanol and methanol extracts of local plants (Berberies lyceum and Fagonia cretica) were tested in vitro against Brucella melitensis via well diffusion method for their antibacterial activity. In in-silico study, phytochemicals previously identified in the selected plants were docked with homology model of the cytotoxic factor malate synthase G (MSG) highly conserved among Brucella spp., in Molecular Operating Environment (MOE) to predict a potential drug against B. melitensis. Molecular dynamic simulation was performed to predict the stability of MSG through MOE. Result Ethanolic crude extracts of B. lyceum showed maximum zone of inhibition (32.5 mm) followed by methanolic extracts (30 mm), while ethanolic extracts of F. cretica showed zone of inhibition (29 mm) followed by methanolic extracts (27.5 mm). In silico screening predicted phytic acid as the most potent inhibitor followed by jehlumine, barbamine, oxyberberine and sindamine. Conclusion The synergistic utilization of phytochemicals derived from B. lyceum may potentially provide protection against B. melitensis.