scispace - formally typeset
Search or ask a question
Topic

Fatty acid-binding protein

About: Fatty acid-binding protein is a research topic. Over the lifetime, 1721 publications have been published within this topic receiving 81530 citations. The topic is also known as: FABP.


Papers
More filters
Journal ArticleDOI
TL;DR: In this article, the effect of age on the expression of the cytosolic fatty acid binding proteins (FABPs) or their content in brain sub-fractions was examined and it was shown that FABP expression was significantly reduced in aged mouse brain.
Abstract: Brain membrane lipid fatty acid composition and consequently membrane fluidity change with increasing age. Intracellular fatty acid binding proteins (FABPs) such as heart H-FABP and the brain specific B-FABP, detected by immunoblotting of brain tissue, are thought to be involved in fatty acid uptake, metabolism, and differentiation in brain. Yet, almost nothing is known regarding the effect of age on the expression of the cytosolic fatty acid binding proteins (FABPs) or their content in brain subfractions. Electrophoresis and quantitative immunoblotting were used to examine the content of these FABPs in synaptosomes in brains from 4, 15, and 25 month old C57BL/6NNia male mice. Brain H-FABP and B-FABP were differentially expressed in mouse brain subcellular fractions. Brain H-FABP was highly concentrated in synaptosomal cytosol. The level of brain H-FABP in synaptosomes, synaptosomal cytosol, and intrasynaptosomal membranes was decreased 33, 35, and 43%, respectively, in 25 month old mice. B-FABP was detected in lower quantity than H-FABP. More important, B-FABP decreased in synaptosomes, synaptic plasma membranes, and synaptosomal cytosol from brains of 25 month old mice. In contrast to H-FABP, B-FABP was not detectable in the intrasynaptosomal membranes in any of the three age groups of mice. In conclusion, expression of both H-FABP and B-FABP was markedly reduced in aged mouse brain. Age differences in brain H-FABP and B-FABP levels in synaptosomal plasma membranes and synaptosomal cytosol may be important factors modulating neuronal differentiation and function.

51 citations

Journal ArticleDOI
TL;DR: Intact proteins were detected in characteristically low charge states, indicating that the proteins remain folded in mouse brain and liver tissue, and native LESA ion mobility spectrometry is presented, showing that the collision cross sections of proteins extracted from tissue may be determined by travelling wave ion mobilitySpectrometry.

51 citations

Journal ArticleDOI
TL;DR: It is demonstrated that the FABP5 gene is upregulated in colorectal cancer cells compared to normal colon cells in a manner that correlates with disease stage and that FABp5 significantly promotes coloreCTal cancer cell growth and metastatic potential.

51 citations

Book ChapterDOI
TL;DR: Titration calorimetry has been evaluated as a method for obtaining binding constants and thermodynamic parameters for the cytosolic fatty acid- and lipid-binding proteins and distinguished affinity classes whose dissociation constants differed by an order of magnitude or less.
Abstract: Titration calorimetry has been evaluated as a method for obtaining binding constants and thermodynamic parameters for the cytosolic fatty acid- and lipid-binding proteins An important feature of this method was its ability to accurately determine binding constants in a non-perturbing manner The equilibrium was not perturbed, since there was no requirement to separate bound and free ligand in order to obtain binding parameters Also, the structure of the lipidprotein complex was not perturbed, since native ligands were used rather than non-native analogues As illustrated for liver fatty acid-binding protein, the method distinguished affinity classes whose dissociation constants differed by an order of magnitude or less It also distinguished endothermic from exothermic binding reactions, as illustrated for the binding of two closely related bile salts to ileal lipid-binding protein The main limitations of the method were its relatively low sensitivity and the difficulty working with highly insoluble ligands, such as cholesterol or saturated longchain fatty acids However, the signal-to-noise ratio was improved by manipulating the buffer conditions, as illustrated for oleate binding to rat intestinal fatty acid binding protein Binding parameters are reported for oleate interactions with several wild-type and mutant lipid-binding proteins from intestine Where possible, the binding parameters obtained from calorimetry were compared with results obtained from fluorescence and Lipidex binding assays of comparable systems

51 citations

Journal ArticleDOI
TL;DR: H-FABP in brain participates in neurite formation and synapse maturation, and may be related to the similar pattern of expression of GABA related markers.

51 citations


Network Information
Related Topics (5)
Signal transduction
122.6K papers, 8.2M citations
83% related
Gene expression
113.3K papers, 5.5M citations
83% related
Inflammation
76.4K papers, 4M citations
83% related
Receptor
159.3K papers, 8.2M citations
82% related
Apoptosis
115.4K papers, 4.8M citations
82% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202368
202272
202142
202044
201950
201851