Flavanone

About: Flavanone is a research topic. Over the lifetime, 1965 publications have been published within this topic receiving 54729 citations. The topic is also known as: flavanones.

Naringin levels in citrus tissues : I. Comparison of different antibodies and tracers for the radioimmunossay of naringin.

Abstract: The preparation of a tritiated radiotracer that was used in the radioimmunoassay of naringin (naringenin-7-O-α-rhamnosyl- (1-2)-β-d-glucopyranoside) and which was synthesized by reduction of the carbonyl group of the flavanone is reported. The resulting assay has a detection limit of 0.5 picomole per 0.1 milliliter, is specific for the 7-neohesperidoside substitution on flavanones, and can measure naringin in crude extracts of plant tissues. This radioimmunoassay is compared with three other naringin immunoassays which use antibodies raised against two different haptens and different tracers labeled with 125I or 3H. The applicability of the methods to the quantification of naringin and other flavanone neohesperidosides in citrus tissue is discussed.

Salicylic acid-induced changes in physiological parameters and genes of the flavonoid biosynthesis pathway in Artemisia vulgaris and Dendranthema nankingense during aphid feeding.

Abstract: Phloem-feeding aphids cause serious damage to plants. The mechanisms of plant-aphid interactions are only partially understood and involve multiple pathways, including phytohormones. In order to investigate whether salicylic acid (SA) is involved and how it plays a part in the defense response to the aphid Macrosiphoniella sanbourni, physiological changes and gene expression profiles in response to aphid inoculation with or without SA pretreatment were compared between the aphid-resistant Artemisia vulgaris 'Variegata' and the susceptible chrysanthemum, Dendranthema nankingense. Changes in levels of reactive oxygen species, malondialdehyde (MDA), and flavonoids, and in the expression of genes involved in flavonoid biosynthesis, including PAL (phenylalanine ammonia-lyase), CHS (chalcone synthase), CHI (chalcone isomerase), F3H (flavanone 3-hydroxylase), F3'H (flavanone 3'-hydroxylase), and DFR (dihydroflavonol reductase), were investigated. Levels of hydrogen peroxide, superoxide anions, MDA, and flavonoids, and their related gene expression, increased after aphid infestation and SA pretreatment followed by aphid infestation; the aphid-resistant A. vulgaris exhibited a more rapid response than the aphid-susceptible D. nankingense to SA treatment and aphid infestation. Taken together, our results suggest that SA could be used to increase aphid resistance in the chrysanthemum.

CCC Sample Cutting for Isolation of Prenylated Phenolics from Hops

Abstract: One of the most potent estrogenic substances from the plant kingdom is the prenylated flavanone, 8‐prenylnaringenin (8PN), present in hops (Humulus lupulus L., Cannabaceae) in low (<10 ppm) concentrations. The prenylated chalcone desmethylxanthohumol (DMX), which is also present in this plant material, serves as a precursor to 8PN. A method for the preparative isolation of these and related bioactive phytochemicals has been developed that involves the use of multiple chromatographic techniques including complementary countercurrent chromatography (CCC) solvent systems. This paper describes the isolation of 8PN and DMX employing “sample‐cutting CCC”. The Hexanes‐EtOAc‐MeOH‐H2O (HEMWat) solvent systems found to be effective for isolation of prenylated phenolics from hops range from HEMWat 8‐2‐8‐2 for diprenylxanthohumol and 6,8‐diprenylnaringenin, to HEMWat 6‐4‐6‐4 for xanthohumol (XH), 6PN and 8PN, to HEMWat 5‐5‐5‐5 for DMX, hydroxylated derivatives of XH, and humulinones.