Topic
Fourier transform spectroscopy
About: Fourier transform spectroscopy is a research topic. Over the lifetime, 5418 publications have been published within this topic receiving 134133 citations.
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TL;DR: Experimental conditions that preserve the original distribution of bound and unbound protein in solution into the gas phase, such that the nanoES mass spectrum provides a quantitative measure of the solution composition, were identified.
Abstract: The influence of solution pH, analyte concentration and in-source dissociation on the measurement of the association constant for a single chain variable fragment of a monoclonal antibody (scFv) and its native trisaccharide ligand by nanoelectrospray-Fourier transform ion cyclotron resonance mass spectrometery has been systematically investigated. From the results of this study, experimental conditions that preserve the original distribution of bound and unbound protein in solution into the gas phase, such that the nanoES mass spectrum provides a quantitative measure of the solution composition, were identified. These include the use of short spray durations (<10 min) to minimize pH changes, equimolar concentrations of protein and ligand to minimize the formation of nonspecific complexes, and short accumulation times (<2 s) in the hexapole of the ion source to avoid collisional heating and dissociation of the gaseous complex. Application of this methodology to the scFv and a series of carbohydrate ligands...
147 citations
TL;DR: In this article, the absorption spectrum of natural water vapour around 1.5μm has been recorded with a typical sensitivity of 5 × 10−10−10 cm−1 by using a CW-cavity ring down spectroscopy set up based on fibred DFB lasers.
147 citations
TL;DR: In this article, an ellipsometry technique based upon on-line Fourier analysis of the transmitted light intensity of a rotating-analyzer ellipsometer by means of a minicomputer is presented and discussed.
146 citations
TL;DR: Extension de la gamme dynamique par ejection selective des ions les plus abondants suivie par excitation normale and detection des ions restants.
Abstract: Extension de la gamme dynamique par ejection selective des ions les plus abondants suivie par excitation normale et detection des ions restants
145 citations
145 citations