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Fourier transform spectroscopy
About: Fourier transform spectroscopy is a research topic. Over the lifetime, 5418 publications have been published within this topic receiving 134133 citations.
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TL;DR: In this paper, the solid phases of indigo, thioindigo, selenoindigo and N,N'-dimethylindigo were recorded with excitation using Nd:YAG laser radiation at 1064 nm.
Abstract: Near-infrared Fourier transform Raman spectra (3400 to — 20 cm−1) for the solid phases of indigo, thioindigo, selenoindigo and N,N'-dimethylindigo were recorded with excitation using Nd:YAG laser radiation at 1064 nm. A complete vibrational assignment is proposed supported by the results of ab initio calculations. The spectra are complementary to the IR spectra recorded earlier. They also complete earlier results of resonance Raman spectroscopy. The spectra support a molecular structure of the indigo chromophore with a cross-conjugated vinylogue amide system.
98 citations
TL;DR: In this article, the spectra of DNA samples have been measured in liquid phase (gel) over the spectral range 10 − 25 cm−1 and compared with spectra obtained from solid films.
Abstract: Significant progress has been achieved during the last several years relating to experimental and theoretical aspects of terahertz (or submillimetre wave) Fourier transform spectroscopy of biological macromolecules. However, previous research in this spectral range has been focused on bio-materials in solid state since it was common opinion that high water absorption will obscure the spectral signatures of the bio-molecules in solutions. At the same time, the biological functions of DNA and proteins take place in water solutions. In this work, the spectra of DNA samples have been measured in liquid phase (gel) over the spectral range 10–25 cm−1 and compared with spectra obtained from solid films. The results demonstrate that there is very little interference between the spectral features of the material under test and the water background except for the band around 18.6 cm−1. Multiple resonances due to low frequency vibrational modes within biological macromolecules in solutions are unambiguously demonstrated. Higher level of sensitivity and higher sharpness of vibrational modes are observed in the liquid environment in comparison with the solid phase, with the width of spectral lines 0.3–0.5 cm−1. Gel sample spectra are found to be polarization-dependent. The ability of THz spectroscopy to characterize samples in liquid phase could be very important since it permits examination of DNA interactions in real (wet) samples. One demonstrated example of practical importance is the ability to discriminate between spectral patterns for native and denaturated DNA.
98 citations
TL;DR: In this paper, a commercial Fourier transform ion cyclotron resonance mass spectrometer utilizing two different configurations: heated filament electron source and an indirectly heated hollow dispenser cathode electron source.
Abstract: Electron detachment dissociation (EDD), recently introduced by Zubarev and co-workers for the dissociation of multiply charged biomolecular anions via a radical ion intermediate, has been shown to be analogous to electron capture dissociation (ECD) in several respects, including more random peptide fragmentation and retention of labile posttranslational modifications. We have previously demonstrated unique fragmentation behavior in ECD compared to vibrational excitation for oligodeoxynucleotide cations. However, that approach is limited by the poor sensitivity for oligonucleotide ionization in positive ion mode. Here, we show implementation of EDD on a commercial Fourier transform ion cyclotron resonance mass spectrometer utilizing two different configurations: a heated filament electron source and an indirectly heated hollow dispenser cathode electron source. The dispenser cathode configuration provides higher EDD efficiency and additional fragmentation channels for hexamer oligodeoxynucleotides. As in ...
98 citations
TL;DR: In this article, a two-dimensional Fourier transform was used to detect the relative sign of the spin coupling constants in high-resolution NMR spectra, which can be used as a diagnostic tool in the recognition of geminal and vicinal proton-proton couplings.
97 citations
TL;DR: The successful development and implementation of a laser ablation source-equipped Fourier transform microwave spectrometer capable of observing 4 GHz regions of spectra in a single data acquisition event is described.
Abstract: The ablation of metal surfaces in the presence of a precursor gas produces reaction products which are often difficult to predict and highly dependent on ablation conditions. This article describes the successful development and implementation of a laser ablation source-equipped Fourier transform microwave spectrometer capable of observing 4GHz regions of spectra in a single data acquisition event. The dramatically increased speed with which regions may be searched, when compared to other high resolution microwave techniques, allows the source conditions to be the prime variable in laser ablation microwave spectroscopic studies. A second feature of the technique is that observed spectral features have correct relative intensities. This is advantageous when assigning observed spectra. The study of two metal chlorides, AgCl and AuCl, illustrate the instrument’s benefits.
96 citations