Topic
Friend leukemia
About: Friend leukemia is a research topic. Over the lifetime, 319 publications have been published within this topic receiving 7463 citations.
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TL;DR: The incorporation of bromodeoxyuridine into DNA resulted in a lowered induction of hemoglobin synthesis in dimethyl sulfoxide-treated cells, and the possibility that this fraction of DNA, which is low in globin gene content, carries genes regulating the erythroid differentiation or the expression of globin genes is discussed.
6 citations
Journal Article•
TL;DR: Ditercalinium, rather than stabilizing DNA as do monointercalators, increased the sensitivity of DNA in situ to denaturation induced by acid, suggesting that the cytokinetic effects and interaction with chromatin of an agent that has the ability to bisintercalate into DNA are qualitatively different from those induced by classical Monointercalating drugs.
Abstract: Ditercalinium (NSC 335153) is a novel 7H-pyridocarbazole dimer in which the two monomers are joined by a rigid bis(ethylpiperidinyl)-linking chain producing a molecule capable of bisintercalation into DNA with extremely high affinity. The effect of ditercalinium on cell proliferation and its interaction with DNA in situ has been investigated in the Friend leukemia cell system. Ditercalinium caused an inhibition of cell growth at 0.5 microM and cell death at 2.5 microM. However, both the cytokinetic and cytotoxic effects became evident only after 1-2 days of continuous drug exposure. In contrast, monointercalators generally affect cell growth within several hours of administration. Furthermore, whereas most intercalators arrest cells in G2 phase, ditercalinium demonstrated no cell cycle phase specificity. In fact, a stathmokinetic experiment, in which vinblastine was used to prevent cell division in exponentially growing Friend leukemia cell cultures, demonstrated that ditercalinium effectively "froze" cells in position throughout the cell cycle, in a dose-dependent fashion. By determining the sensitivity of DNA in situ in fixed Friend leukemia cells to acid-induced denaturation, it was apparent that ditercalinium, rather than stabilizing DNA as do monointercalators, increased the sensitivity of DNA in situ to denaturation induced by acid. It appears, therefore, that the cytokinetic effects and interaction with chromatin of an agent that has the ability to bisintercalate into DNA are qualitatively different from those induced by classical monointercalating drugs.
6 citations
TL;DR: The bone marrow cells from lead-poisoned rats exhibited a lag in onset of maturation, which was consistent with the effect of lead on Friend cells, while synthesis of this enzyme increased in the presence of lead.
6 citations
TL;DR: In this article, the authors present a method for purification and analyses physicochimiques de l'anticancereux produit par streptomyces sioyaensis.
Abstract: Purification et analyses physicochimiques de l'anticancereux produit par streptomyces sioyaensis
6 citations
TL;DR: This non‐virus‐producing, mouse erythroleukemia cell line will be useful for the study of mutated p53 function during the induction of erystrodifferentiation or apoptotic change.
Abstract: Two different erythroleukemia cell lines have been established from the splenic lesions of transgenic mice possessing the Friend spleen focus-forming virus (F-SFFV) gp55 gene. One showed a neardiploid karyotype and a temperature-sensitive (ts) p53 mutation, and the other, a hyper-triploid karyotype with double p53 mutations found by single-strand conformation polymorphism (SSCP) analysis. The cell lines both retained No.11 chromosomes on which p53 genes are localized. Another p53 allele in the cell line with the ts-p53 mutation appeared intact in the SSCP analysis of the genomic exon 5. The cells with the ts-mutant p53 gene showed no apparent change with temperature shift in their growth or dimethylsulfoxide-induced differentiation, although the wild-type p53 gene on the other allele was not expressing. This ts-p53val-135 gene made p53-deficient fibroblasts anchorageindependent at 37°C but not at 32°C. This non-virus-producing, mouse erythroleukemia cell line will be useful for the study of mutated p53 function during the induction of erythrodifferentiation or apoptotic change.
6 citations