Friend leukemia

About: Friend leukemia is a research topic. Over the lifetime, 319 publications have been published within this topic receiving 7463 citations.

Translation level control in normal and leukemic cells.

Abstract: A useful model for consideration of the biochemical aspects of cancer holds the primary lesion to be a block in the normal processes of differentiation. Erythroleukemia induced in susceptible mice by Friend leukemia virus appears to fit this model which is illustrated in Figure 1. This virus blocks the differentiation of erythroid cells at the proerythroblast stage (1, 2) before any appreciable synthesis of hemoglobin takes place (3). Friend virus transformed proerythroblasts from leukemic mice can be carried as permanent lines and are easily propagated in suspension culture. Differentiation as reflected by hemoglobin synthesis may be induced in vitro by the simple expedient of adding certain aprotonic solvents, such as dimethylsulfoxide (DMSO), to the tissue culture nutrient in which the Friend leukemia cells (FLC) are grown (3–5). Thus, these cell lines provide an excellent system for the study of both transcriptional and translational control mechanisms involved in differentiation leading to the synthesis of hemoglobin. A better understanding of these controls eventually could lead to the manipulation of the regulatory elements involved in leukemia.

Possible Role of the Friend Virus Life Cycle in Differentiating Friend Leukemia Cells Treated with Interferon

Abstract: Friend erythroleukemia cells (FLC) are proerythroblasts chronically infected with the Friend virus complex (FLV, composed by LLV and SFFV). They undergo erythroid differentiation accompanied by synthesis of heme and hemoglobin (Hb), accumulation of globin mRNA and erythrocyte-specific membrane changes upon treatment with DMSO and other polar solvents [6].

Differentiation of Friend virus-induced leukemia cells.

Abstract: A long-term cultured Friend leukemia cell is able to differentiate along the erythrocytic series following treatment with some substances and that can be demonstrated by a conjugated erythrocyte membrane-specific antibody technique. Differentiation is induced by inhibition of DNA synthesis and by dimethylsulfoxide (DMSO), erythropoietin (EP) and Vitamin B12 (B12), and other agents. The effective substances were divided into 2 groups by the mode of differentiation of Friend cells. A differentiated state continues for several days when cells are re-cultured in the medium without substances. The decreased tumorigenecity of differentiated cells is proved by back transplantation to mice.

Characteristics of four Friend leukemia cell sublines resistant to adriamycin.

Abstract: Resistance of Friend murine erythroleukemia cells was induced or selected by continuous stepwise exposure to adriamycin (ADM). The resistance index (R.I.) varied with different "mdr type" drugs, even when the compounds were closely related as ADM and daunorubicin (DNR). The cell uptake of anthracycline, evaluated by flow cytometry (FCM), was better correlated with the R.I. than the HPLC assessment. The quantitative cytology showed nuclear changes (size and color distribution); all the modifications were in part dependent on the degree of resistance. This study showed that the graded resistant sublines differed in their resistant phenotypes apart from their different degree of resistance.