Friend leukemia

About: Friend leukemia is a research topic. Over the lifetime, 319 publications have been published within this topic receiving 7463 citations.

Specific inducibility of globin mRNA in Friend leukemia cells in low serum concentration.

Abstract: Friend leukemia cells were adapted to grow in a medium containing 0.5% serum by gradually decreasing concentration of the serum from 15%. Upon treatment of the cells with dimethyl sulfoxide, appearance of benzidine-positive cells was suppressed in the cells cultivated in a 0.5% serum medium, whereas globin mRNA sequences, as determined by hybridization of cytoplasmic RNA with globin cDNA, increased to the same order as in the cells grown in a medium containing 15% serum. A small but significant amount of globin was synthesized in cells treated with dimethyl sulfoxide in 0.5% serum medium, as determined by dodecyl sulfate-polyacrylamide gel electrophoresis of 3H-labeled cellular proteins.

Hemopoietic stem cells in the liver of mice with friend leukemia

Abstract: Hemopoietic stem cells were studied in the marrow, spleen and liver of individual, FV-P infected DBA/2 mice. At more than 17 days after infection there was a tendency for CFU-EI numbers in the spleen to be reduced compared to earlier intervals after virus infection; CFU-EI numbers in the marrow were low. At the same time all hemopoietic stem cells (CFU-S, CFU-C, BFU-E and CFU-EI) could be found in the enlarged liver. The migration of stem cells from the marrow to the spleen and further to the liver is discussed.

In vitro and in vivo studies of poly-L-lysine as inducer of friend leukemic cells differentiation

Abstract: Poly-L-lysine, a synthetic cationic polypeptide known for its ability to bind to cell membranes, was found to induce differentiation of Friend leukemia cells "in vitro". Studies were extended to the same "in vitro" model, in order to examine the therapeutic potential of this new differentiating agent. The i.p. administration of the polymer (Mw 2700) at the maximal tolerated dose resulted in major alterations of disease-related parameters. In particular, a multiple treatment schedule on the advanced disease resulted in a successful reduction of target organ weight and peripheral white blood cell count and appreciable differentiation of spleen and bone marrow cells. Apparently, the effects of poly-L-lysine were superior to those produced by N-methyl-acetamide, a potent inducer of differentiation "in vitro".

Preparations on development of friend leukemia in mice.

Abstract: Summary Administration of concentrated preparations of interferon of high titer (extracted from either mouse brain or serum and spleen) inhibited the development of splenomegaly induced by Friend virus in Swiss and DBA2 mice. Interferon was effective only when treatment was continued daily throughout the duration of the experiment, and when inoculated mtraperitoneally (rather than intravenously). We acknowledge with gratitude the excellent assistance of Mile. Chantal Bourali and Mile. Marie-Thé rèse Thomas. Dr. Raymond Latarjet kindly supplied us with the Friend virus and with a large number of Swiss mice which permitted us to start a colony of these mice. We are indebted to Dr. Charles Chany and to Dr. Rebecca Falcoff for discussion and help in preparation of this report, and to Mr. Philippe Lazar and Mrs. Suzanne Gué guen for analyzing our experimental results and aiding us in their presentation. We should like to thank Dr. John F. Enders for reviewing this manuscript and Dr. Sidney Farber for constant encouragement and advice.