Friend leukemia

About: Friend leukemia is a research topic. Over the lifetime, 319 publications have been published within this topic receiving 7463 citations.

H-2 Antigen Variants in a Cultured Heterozygous Mouse Leukemia Cell Line. IV. Cell=Mediated Cytolysis of Wild Type and Variant Cells

Abstract: H-2 antigen variants, derived from a heterozygous mouse Friend leukemia cell line by selection with anti-H-2 antisera and complement, were tested for susceptibility to cell-mediated cytolysis, using T-lymphocytes directed against individual H-2 antigens. The cytotoxic cells were generated in the BALB and B 10 backgrounds by a combination of in vivo and in vitro immunizations. The phenotypes of the variants inferred from the patterns of resistance or susceptibility to CML were consistent with those presumed from earlier assays using antisera. The one exception was the variant cell line which was H-2D d- in assays using antisera, but was H-2D d+ by CML.

Isozyme patterns of pyruvate kinase and differentiation of friend leukemia cells

Abstract: The isozyme patterns of glycolytic enzymes of Friend leukemia cells (FLC) were compared with those of erythrocytes and erythroblasts. Erythrocyte-specific R types of pyruvate kinase (PK) were clearly observed in phenylhydrazine-induced mouse erythroblast, and much less amount of them was also observed in Friend leukemia cells. When FLC were induced to differentiate by hexamethylene-bisacetamide (HMBA), the R types were slightly reduced. When the induction of differentiation was inhibited by 12-O-tetradecanoylphorbol 13-acetate (TPA), the R types and M2-R hybrids rather increased. These results are reverse of those obtained when hemoglobin production is used as a marker of differentiation. Isozyme patterns of lactic dehydrogenase and aldolase did not change during differentiation of FLC induced by HMBA, and were the same as those of mouse erythroblasts and erythrocytes.