Topic
Gel electrophoresis
About: Gel electrophoresis is a research topic. Over the lifetime, 26026 publications have been published within this topic receiving 1113565 citations.
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TL;DR: The potential of capillary gel electrophoresis with laser induced fluorescence detection as a tool for DNA sequence determination with synthetic oligonucleotides and single-stranded phage DNA as templates is demonstrated.
172 citations
Patent•
08 Jul 1985
TL;DR: In this paper, two proteins that are found in bone and that have in vivo chondrogenic/osteogenic activity in combination with a co-factor are described, which indicate that the proteins are probably homodimers.
Abstract: Two proteins that are found in bone and that have in vivo chondrogenic/osteogenic activity in combination with a co-factor are described. Both proteins also were active in combination with EGF in the in vitro TGF-β assay. Each has a molecular weight of approximately 26,000 daltons by SDS-PAGE. Each is reduced to a single polypeptide indicating that the proteins are probably homodimers. One has an N-terminal sequence identical to that of human placenta-derived TGF-β whereas the other has an N-terminal sequence that is different from that of TGF-β derived from human placenta. The two proteins may be purified to homogeneity using RP-HPLC or acetic acid-urea gel electrophoresis.
172 citations
TL;DR: It is shown that dialysis permits the analysis of multi-protein complexes of whole cellular lysates by BN-PAGE, and protein complexes of various proteins including the tumor suppressor p53 and c-Myc are identified via mass spectrometry showing that the method has a wide potential for functional proteomics.
172 citations
TL;DR: Following optimization of both translation in the wheat germ system and the conditions during in vitro uptake, the post-translational transport of over 100 polypeptides is observed; many remain in the soluble phase of the organelle while others integrate into the thylakoid membranes.
172 citations
TL;DR: Mouse sperm contain a major phosphotyrosine-containing protein which has been implicated as a sperm membrane receptor for the egg zona pellucida glycoprotein, ZP3 and this protein was purified and subjected to limited tryptic digestion and subsequent amino acid analysis, suggesting that the germ cell component of the testis possesses a unique tyrosine -phosphorylated form of hexokinase.
172 citations