Topic
Gel electrophoresis
About: Gel electrophoresis is a research topic. Over the lifetime, 26026 publications have been published within this topic receiving 1113565 citations.
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TL;DR: The purified phosphoinositide 3-kinase preparation contained an 85-kDa protein and a protein doublet of approximately 110 kDa, which was the same protein previously shown to associate with polyoma virus middle T antigen and the platelet-derived growth factor receptor.
597 citations
TL;DR: Gel electrophoresis of restriction fragments of deoxyribonucleic acid (DNA) in agarose or polyacrylamide gels is discussed, which is often useful to identify a particular sequence in the DNA fragments separated by gel electrophoreis.
Abstract: Publisher Summary This chapter discusses gel electrophoresis of restriction fragments. There are many designs of an apparatus that can be used for the electrophoresis of deoxyribonucleic acid (DNA) in agarose or polyacrylamide gels. Some of them are very easy to make from glass or perspex, and a simple apparatus can give excellent results. It is often useful to identify a particular sequence in the DNA fragments separated by gel electrophoresis. At present, there are a number of methods available to achieve this purpose. Restriction fragments that bind to a protein may be separated as a complex by filtration through a membrane filter. Radioactive restriction fragments that hybridize to a particular ribonucleic acid (RNA) molecule can be measured by gel electrophoresis after nonhybridized parts of the fragments are digested with the single-strand-specific nuclease S1. Alternatively, nonradioactive restriction fragments, after they are separated by agarose gel electrophoresis, can be probed with radioactive RNA or DNA to mark the positions of specific sequences.
590 citations
TL;DR: The brain contains two distinct molecular forms of the (Na,K)-ATPase (sodium and potassium ion-stimulated adenosine triphosphatase) that differ in at least one kinetic parameter: the affinity for the specific inhibitor strophanthidin.
588 citations
TL;DR: The feasibility of high-speed, high-throughput DNA sequencing using capillary array electrophoresis chips is established and the prospects for enhancing the resolution and sensitivity of these chip separations are discussed.
Abstract: DNA sequencing has been performed on microfabricated capillary electrophoresis chips. DNA separations were achieved in 50 x 8 microns cross-section channels microfabricated in a 2 in. x 3 in. glass sandwich structure using a denaturing 9% T, 0% C polyacrylamide sieving medium. DNA sequencing fragment ladders were produced and fluorescently labeled using the recently developed energy transfer dye-labeled primers. Sequencing extension fragments were separated to approximately 433 bases in only 10 min using a one-color detection system and an effective separation distance of only 3.5 cm. Using a four-color labeling and detection format, DNA sequencing with 97% accuracy and single-base resolution to approximately 150 bases was achieved in only 540 s. A resolution of greater than 0.5 was obtained out to 200 bases for both the one- and four-color separations. The prospects for enhancing the resolution and sensitivity of these chip separations are discussed. This work establishes the feasibility of high-speed, high-throughput DNA sequencing using capillary array electrophoresis chips.
580 citations
TL;DR: Recovery of activity could only be achieved when the required components were mixed prior to removal of urea, suggesting that one or more of these proteins may refold improperly on removal of Urea unless the other components are present.
580 citations