Showing papers on "Gelatin published in 1973"

Bone Morphogenesis in Implants of Insoluble Bone Gelatin

Abstract: Insoluble bone gelatin with inclusions of insoluble noncollagenous protein produces new bone when implanted in muscle in allogeneic rats. The implanted residue provides the milieu for expression of bone morphogenetic potential of migratory mesenchymal cells. Neutral buffer solutions activate endogenous enzymes that degrade components essential for cell interactions and differentiation of bone. Chloroform-methanol either denatures or extracts constituents responsible for degradation. Insoluble bone gelatin produces new bone after extraction at 2° with neutral salts, 0.5 M EDTA, 0.1 M Tris·HCl, 4 M urea, 0.5 M hydroxylamine, and 10 M KCNS, as well as after limited digestion with pepsin or collagenase, but not after extraction with 5 M guanidine, 7 M urea, water saturated with phenol, or after alkali hydrolysis with 0.1 N NaOH. The specific activity of cell populations interacting with insoluble bone gelatin suggests that a chemical bond between collagen and a noncollagenous protein or part of a protein, cleaved by a neutral proteinase, controls the bone morphogenetic reaction.

Gelatin: A Poor Substrate for a Mammalian Collagenase

Abstract: A rabbit tumor collagenase was purified more than 5000-fold. In this form it degrades native collagen in helical conformation at 37°C, p H 7.6, into two fragments, but it had little capacity to cleave gelatin, an indication of the importance of higher-order structure of substrate for this enzyme in pure form. It is likely that, in vivo, enzymes other than collagenase degrade gelatin polypeptides produced by primary collagenolysis.

Dishwashing compositions containing gel forming gelatin

Abstract: Liquid detergent compositions containing gel-forming gelatin, said compositions being especially suitable for use on kitchen utensils. Said compositions minimize filming and spotting of utensil surfaces upon drying. A method for treating kitchen utensil surfaces with aqueous solutions of gel-forming gelatin is also disclosed.

Preparation of water soluble gelatin

Abstract: Cold water soluble gelatin is prepared by co-drying gelatin with suitable edible acids, wherein the edible acid is present in amounts of from about 30% to 200% by weight of the gelatin.

The reaction of gelatin/gum arabic coacervate gels with glutaraldehyde

Abstract: The consumption of glut by various gelatin/gum arabic (GGA) coacervate gels has been examined over a range of conditions. Both acid- and alkali-precursor gelatins were used to form the coacervates. Glut consumptions by several gelatin gels were also determined. All gels examined consumed 0.3 to 1.6 mmole glut/g gelatin. Acid and alkali GGA gels had similar glut uptakes at 4°C. Glut consumption by acid-precursor GGA gels increased significantly with increasing gelation temperature (4–28°C) due to temperature-dependent changes in gel structure. The use of commercial versus redistilled glut had no detectable effect upon glut uptakes. Reaction of a gel with glut caused a high degree of insolubilization as a result of intermolecular crosslink formation. Most treated gels were at least 85 wt% insoluble at 55°C in phosphate buffer after 4–28 days extraction. That fraction of a gel solubilized by the extraction process contained both gelatin and gum arabic. Thus, despite the fact that gum arabic has little tendency to react with glut, it is effectively entrapped in the crosslinked GGA gel structure. Gels with lowest glut uptakes were the most soluble ones. They also were the only ones with solubilities that increased upon treatment with NaHSO 3 before the gel fraction extraction process. Insolubilization of GGA gels by glut causes their swelling behavior and composition to become essentially independent of pH. Composition and swelling behavior of uncrosslinked gels vary markedly with pH due to the reversible nature of the intermolecular bridges responsible for gelation.

Separation of platelet rich plasma and red cells with modified gelatin.

Abstract: . The l'reparation of platelet rich plasma (PRP) by acceleration of red cell sedimentation with modified gelatin is evaluated. The mean recordings in the supernatants of 25 blood units after a sedimentation time of 92 min are a platelet yield of 88% and less than 1% of red cells in 60% of the phlebotomy volume. The addition of modified gelatin to freshly drawn blood did not alter the platelet function or viability as measured by in vitro aggregation to ADP, adhesion to glass bead columns, bleeding time following a load of 1,500 ml in surgical patients and survival in normal recipients after storage at 22 °C to 72 h. PRP preparation with modified gelatin necessitates a minimal mechanical equipment and may therefore prove particularly valuable for platelet substitution in non-hospitalized patients.

Printing method and system by gelatin coagulation

Abstract: A method and a system adapted for high speed image reproduction or surface coating by electro-osmosis drying of a colloid. A thin layer containing a colloid (gelatin or albumin), water and a salt selected from chlorides and sulfates, is sandwiched between at least one pair of opposite electrodes, and an electrical bias on the latter causes the colloid to coagulate and adhere to one electrode, thereby forming a coating or an imprint. A coating of highly uniform thickness can be obtained and which may be varied by adjusting the quantity of current passed through the layer. An image may be electrically produced by sandwiching such layer between an electrode means, and a plurality of electricallyinsulated juxtaposed electrodes. The temperature of the layer is controlled so that the gelatin remains liquid and spatially selected ones of the plurality of electrodes are electrically biased with respect to the electrode means to pass electric pulses through the layer, whereby coagulation adherence of gelatin is produced at points adjacent to each biased electrode. The coagulated gelatin adheres to the positive electrodes and the remaining non-coagulated liquid layer is thereafter removed, thus forming a printing plate. The pulsations of the electrical signal may be of different amplitudes from one electrode to the other, in accordance with the shades or light of the image to be reproduced, whereby to produce a corresponding variation in the amount of coagulated gelatin resulting in a printing plate which reproduces the half-tones of the image. A very thin coating can be obtained of the order of several thousand Angstroem units; thus, polychromatic reproduction can be obtained using the principle of interferential light reflection.

Adhesion of hydrophilic layers on polyester film

Abstract: The adhesion of hydrophilic layers on a dimensionally stable polyester film support is improved by applying after longitudinal stretching of the polyester film a single adhesive layer thereon. The thus covered polyester film is stretched in transverse direction and heat-setted at 180 DEG -220 DEG C. The adhesive layer comprises 30 to 80 % by weight of a chlorine-containing copolymer, 5 to 30 % by weight of gelatin, 5 to 40 % by weight of a plasticizer for the gelatin and 0 to 30 % by weight of a metal-complexing antistatic agent.

Method for the preparation of medicinal capsules

Abstract: A method for the preparation of gelatin capsules having a good disintegrating property, in which a material selected from the group consisting of a protein, an amino acid, a substance having at least one amino group, an antioxidant, monobasic sodium phosphate, and the mixture thereof is added to the encapsulated contents.

The effects of gelatin on aggregation of protoplasts from higher plants.

Abstract: Aggregation of protoplasts of Allium fistulosum L., Brassica chinensis L. and Daucus carota L. was induced in great frequency with gelatin and early products of gelatin degradation. The optimal gelatin concentration was 2–5%. The aggregated protoplasts fused into groups and cell walls were formed around them in culture.

Dielectric relaxation of collagen and gelatin

Abstract: Dielectric dispersions of reconstituted collagens and gelatin were measured from 0.1 to 10 kHz and −160 to +160°C. At 0.1 kHz there is a γ transition at −80°C which is attributed to the H2O-coupled local modes. The process has an activation energy of 7.5 kcal. A devitrification process is observed at 10–20°C. Both of these processes have their counterparts in the dynamic mechanical measurements. The tan δ values are up to 3 times as great for the dynamic mechanical dispersions. There is an additional hightemperature dielectric loss transition which does not correspond to any seen with the mechanical experiments. A probable mechanism for this absorption is the Maxwell-Wagner-Sillars effect.

Improvement in viscosity of gelatin solutions for photosensitive materials

Abstract: Photographic elements having a protective coating or photosensitive layer with improved viscosity characteristics and improved conductivity are provided by incorporating into a gelatin containing coating selected hydrophilic polymers and a soluble inorganic fluoride salt. The elements thus improved conventionally contain a support, one or more photographic silver halide layers and one or more protective layers. This improved composition may be, for example, a conventional silver halide emulsion formulated with gelatin and including the sodium salt of polystyrene sulfonate and ammonium fluoride, or a conventional protective overcoat composition with gelatin, the usual coating aids and hardeners and including the potassium salt of polystyrene sulfonate and ammonium silico fluoride.

Cold water-soluble gelatin composition and process of preparing same

Abstract: A cold water-soluble gelatin composition is prepared by subjecting a sugar/gelatin admixture to heat and pressure while applying shearing forces to the admixture in a closed system.

The Mammalian Intervertebral Disc. The Collagen of Whale Fetal Nucleus Pulposus

Abstract: The hydroxyproline content and total amount of imino acids of whale nucleus collagen are in the range of variability usually observed in other mammalian collagen and about the same as the values reported for the collagen isolated from human intervertebral disc.Salt and acid extracted collagen of fetal whale nucleus pulposus was obtained with the aid of pronase. Soluble collagen preparations were also obtained without the use of pronase. Insoluble collagen was purified as gelatin. The amino acid and carbohydrate composition of these preparations was essentially the same. The salt-soluble collagen of the fetal nucleus contains 2% neutral carbohydrate, present as galactose and glucose in 1.5:1 ratio, trace amounts of hexosamine and no uronic acid. The galactose to glucose ratio is, however, higher in gelatin preparations.Carboxymethylcellulose chromatography of neutral salt-extracted collagen (after pronase) primarily showed two chromatographic components corresponding to αl and β11 chains of rat tendon solu...

Glass fiber size and resulting product

Abstract: A method of producing an improved sized glass fiber chopped strand suitable for the manufacture of articles formed from aqueous dispersion is shown wherein glass fiber filaments are treated with an aqueous sizing solution comprising gelatin, an nalkyl N-amido-alkyl imidazoline and a fatty acid amide.

Method of preparing silver catalyst

Abstract: Silver catalysts of uniform fine particle size, having improved activity in the oxidation of ethylene to ethylene oxide, have been prepared by depositing silver or a silver compound on a carrier in the presence of dispersing agents selected from certain natural gums, e.g. karaya or gelatin.

Texture of SS Cross-linked Gelatin Gels

Abstract: Relations among sensory properties, physical characteristics and the kinds of force supporting gel structure were studied by using thiolated gelatin.Breaking stress of the gelatin gel was greatly influenced by contents of disulfide bonds in the gel. Young’s modulus of the gel was affected by its temperature. As to sensory properties of the gelatin gel, “hardness” highly correlated with Young’s modulus and “brittleness” correlated with breaking stress.Therefore, it could be concluded that in the case of the gelatin gel, “hardness” was mainly attributed to hydrogen bonds and “brittleness” was to chemical bonds like disulfide linkages.

Process for preparing low calorie gelatin dessert with bromelain

Abstract: Novel low calorie gelatin desserts and gelatin based puddings which contain small amounts of bromelain and/or papain to provide a smooth texture and yield desserts which do not harden, become rubbery, or crack when stored over extended periods of time.

Hydrophilic colloid silver halide emulsion hardened with a bis(vinyl-sulfonylmethyl) ether and an acrylic compound

Abstract: Certain blends of A. ACRYLIC HARDENER, PLUS B. BIS(VINYLSULFONYLMETHYL)ETHER EXHIBIT SYNERGISM WITH RESPECT TO CONTROLLING THE "AFTERHARDENING" THAT IS USUALLY OBSERVED WHEN, FOR EXAMPLE, GELATIN-CONTAINING COMPOSITIONS ARE CHEMICALLY HARDENED. The hardened gelatin compositions are particularly useful as emulsion layers in photographic elements.

Process for reducing sticking of blood cells to glass in blood testing procedures

Abstract: Blood Cells are suspended in a physiological saline solution modified by the addition of a small amount of gelatin. The presence of gelatin serves to reduce attractive forces between the blood cells and glass surfaces in testing procedures.

Process for preparing microcapsules containing hydrophobic oil drops

Abstract: In a process for producing microcapsules containing hydrophobic oil drops by complex coacervation including a conventional prehardening treatment in which an aldehyde and an alkali material are added to the system, the improvement which comprises adding gelatin having an isoelectric point lower than that of the gelatin employed for forming the walls of the coacervates to the system at a temperature lower than the gelling point of the gelatin employed for forming the walls of the coacervates, but prior to the simultaneous presence in the system of said aldehyde and alkali material, in an amount sufficient to prevent an increase in viscosity due to the reaction of gelatin and aldehyde during said prehardening step.

Low calorie, cold water soluble quick set gelatin dessert

Abstract: A cold water soluble, heat-reversible quick setting gelatin is made by co-drying gelatin with polyglucose along with other ingredients of a gelatin dessert type composition.