Showing papers on "Gelatin published in 1977"

Binding of soluble form of fibroblast surface protein, fibronectin, to collagen.

Abstract: Fibronectin, a plasma protein immunologically identical with a major surface protein of normal fibroblasts, was found to bind to collagen and gelatin. A solid phase enzyme immunoassay was used for the binding tests. Collagen, gelatin or various control proteins were adsorbed to a plastic surface. Binding of fibronectin was detected using purified fibronectin antibodies conjugated to alkaline phosphatase. Circulating fibronectin and fibronectin obtained from fibroblast cultures both showed specific binding to collagen and gelatin. Preparative affinity chromatography of plasma on gelatin coupled to Sepharose gave electrophoretically and immunologically pure fibronectin in high yields. Malignantly transformed fibroblasts lack surface fibronectin. Our findings suggest the possibility that this results in a lack of anchorage to the surrounding intercellular matrix, which could contribute to the malignant growth behavior.

Corrective agent for the covering and/or filling of bone defects, method for the preparation of same and method of using the same

Abstract: A corrective agent used for the covering and/or filling of bone defects, comprising a solution forming a gel at body temperature which comprises a physiological saline solution containing at least one polysaccharide and/or gelatin. Other additives may also be present. The corrective agent can be prepared by boiling the at least one polysaccharide and/or gelatin in the physiological saline solution to dissolve and sterilize. The corrective agent is then generally maintained above the temperature at which solidification to a gel occurs until use, though it may be gelled and once reheated. Other additives may also be present, with time of addition depending upon the thermal susceptibility of the additives. The corrective agent is used by coating the same on the area to be covered and/or filled.

Immobilization of microorganisms in a hydrophilic complex gel

Abstract: Immobilization of microorganisms is carried out by mixing a water soluble-polymer selected from polyvinylalcohol, gelatin and carboxymethylcellulose with a tetraalkoxysilane, hydrolyzing the resulting mixture by the addition of acid to form a homogeneous complex sol, dispersing microbial cells homogeneously in the sol and gelling the mixture of the sol and microbial cells.

Crosslinkable polymers having vinylsulfonyl groups or styrylsulfonyl groups and their use as hardeners for gelatin

Abstract: Polymers containing pendent substituted ethylsulfonyl, vinylsulfonyl or styrylsulfonyl groups are useful as gelatin hardeners or can be crosslinked by bis-nucleophiles or by exposure to ultraviolet light.

Thickened gelatinous edible alcoholic medicated carrier

Abstract: Beverage alcoholic liquid is mixed as a carrier with compatible medicating substances to be taken orally, and the mixture is thickened to a heavy fluid or solid gel by addition of an edible gelating agent such as gelatin, low methoxyl pectin, alkali pectate, carboxy methyl cellulose and methyl cellulose.

Peptide mixtures derived from collagenous material or gelatin

Abstract: Peptide mixtures having relatively narrow average molecular weights and characteristic amino acid compositions and a process for preparing the same from a collagenous material or gelatin are disclosed. The peptide mixtures have unique properties and are useful as a water- or moisture-retaining agent for cosmetic articles or foods, a foaming agent for foods, a bitterness-blocking agent for medicines or a nutritional component for soft drinks.

The coagulation of particles in suspension by freezing-thawing

Abstract: Freeze-thaw coagulation can be prevented by adding gelatin or polymers to the suspension. The effectiveness is due to the prevention of the growth of ice crystals and immobilization of suspended particles by the gel structure or networks caused by gelatin or polymers. Coagulation is also prevented by adjusting the salt concentration of a dispersion medium to an eutectic concentration. Some practical improvements for preventing the freeze-thaw coagulation were given.

Fabrication, characterization, and modification of the texture of calcium alginate gels

Abstract: A simulated fruit gel suitable for freeze dehydration has been developed. The fabricated gel system consists primarily of alginate molecules crosslinked by calcium ions. Static compression tests or compression tests using the Instron Universal Testing Machine were used to characterize the mechanical properties of crosslinked gels having different compositions. It has been observed that incorporation of additional components such as pectin, gelatin, or sucrose to the calcium alginate system modified the textural characteristics of the crosslinked gels. Some hypotheses explaining the effect of added components on gel structure and texture are presented. Comparison of the results obtained from sensory and instrumental methods of texture measurement showed that if the instrumentally measured mechanical properties of two gel samples are different by 20% or more, sensory panelists could differentiate between the two samples at a high level of statistical significance.

Insoluble enzymatically active particles

Abstract: Insoluble enzymatically active particles are produced by forming an intimate mixture of a jellifying protein such as gelatin and microbial cells containing an enzyme such as glucose isomerase, passing the mixture through a die to produce threads, passing the threads into cold water to jellify the threads, contacting the jellified threads with a crosslinking agent and cutting the resultant crosslinked threads into pieces.

Method of hardening gelatin and photographic light-sensitive material

Abstract: A photographic silver halide light-sensitive material having at least one hydrophilic colloid layer containing gelatin and/or a gelatin derivative hardened with a compound represented by the following general formula (I): ##STR1## wherein a and d each represents 1 or 2; b and c each represents 0, 1 or 2 with the proviso that both b and c are not simultaneously 0; R represents a divalent group; and n is 0 or 1, which compound provides a preferred hardening rate and does not adversely affect the photographic properties of photographic light-sensitive materials, and a method of hardening gelatin and/or a gelatin derivative comprising treating the gelatin and/or the gelatin derivative with a compound represented by the general formula (I) above.

Transparent liq. gels e.g. of hydrophilic acrylic! polymer and gelatin - useful as wound dressings, esp. for burns and ulcers, and as cell culture media

Abstract: Transparent liq. dressing, esp. for wounds, comprises a hydrophilic organic transparent gel in sheet or tape form, swollen with an aq. soln. The dressing may be reinforced with a string or net-like reinforcing agent. The aq. soln. used to swell the gel may contain buffers, wound treatment agents, nutrients and/or growth agents. Healing progress of the wound visually without removing the dressing. When it becomes necessary to change it, the old dressing can be removed easily without damage to the new tissue forming underneath. The gels are also useful as cell culture media. Pref. the gel consists of a mixt. of a hydrophilic polymer (I) and >=1 high molecular gel-forming substance. Esp. useful gels are comprised of a gel-forming polysaccharide and/or a protein or polypeptide, and a polymer of a hydrophilic (meth)acrylic acid deriv. made by polymerisation in the presence of the gel-forming substance. Suitable gel-forming substances are e.g., agarose and gelatin.

Stabilized soft gelatin capsule composition of 1 α-hydroxy-vitamin D

Abstract: A soft gelatin capsule preparation of a 1α-hydroxy-vitamin D which contains sorbic acid or its salt and a process for preparing the preparation are disclosed Sorbic acid or its salt which is incorporated in a soft gelatin capsule and/or an oily diluent acts as a preservative for gelatin and as a stabilizer for the vitamin D

Some factors affecting gelatin gel texture evaluation by penetration testing

Abstract: A mechanical tester with punches of different sizes and shapes was used to determine the effect of penetration speed, penetration depth, and gelatin concentration on the resistance of gelatin gels to penetration Penetration speed in the range of 004–012 cm s-1had no significant effect on force readings A straight line relationship existed between the square of gelatin concentration and penetration force Depth of penetration was found to be an important factor in the measurement of the mechanical behaviour of the material Coefficients of determination showed a high correlation between punch shape, circumference, depth of penetration, gelatin concentration and force readings

Collagenase-released non-collagenous proteins of cortical bone matrix.

Abstract: Two distinct groups of non-collagenous components were isolated from rat cortical bone gelatin which had previously been digested with purified bacterial col-lagenase. One component was disulfide-bonded, strongly acidic, trypsin-labile glycoprotein aggregate with a molecular mass of more than 100, 000 daltons. When reduced with β-mercaptoethanol this protein disaggregated into subunits with a molecular mass of about 60, 000 daltons. The other components consisted of a group of polypeptides with a molecular mass of about 5, 000 daltons. The latter group was present in collagenase digests prepared from normal bone gelatin but was hardly detectable or absent in digests of gelatin prepared from either autolyzed, trypsinized or lathyritic bone, or from the residue of neutral salt extracted rat tail tendon. A recently discovered group of non-collagenous proteins is tightly bound to the highly crosslinked insoluble structure of collagen fibrils of bone and dentin. Dische et al 1 predicted the existence ...

Adhesion preventing method for silver halide photographic material

Abstract: PURPOSE: To prevent adhesion without impairing physical properties, such a-stransparency or dimentional resistance after processing and photographic properties, by specifying the gelatin coating amount on a surface layer and the means size of fine particles contained COPYRIGHT: (C)1978,JPO&Japio

Hardening agent for gelatin and method for hardening gelatin

Abstract: A hardening agent for gelatin comprising a compound represented by the formula (I): ##STR1## wherein R represents an n-valent organic group, Z represents an atomic group necessary to complete a 5- or 6-membered ring and n represents 1, 2 or 3; and a method for hardening gelatin comprising treating gelatin with a compound represented by the formula (I) above.

Effect of formulation additives on the dissolution behavior of tetracycline antibiotics.

Abstract: The effect of various formulation additives on the dissolution behavior of three tetracycline antibiotics from compressed tablets (prepared by direct compression) was studied. In general, the dissolution of the antibiotics was found to be retarded by the addition of binding agents such as gelatin and acacia, when they were incorporated in compressed tablets. A study was also undertaken to determine the influence of gelatin and acacia dissolved in the test media. In dissolution experiments on compressed tablets and crystalline powders, a significant dissolution inhibition by the action of the dissolved additives was present, as in the study where additives were incorporated in compressed tablets. It was thought that the possible mechanisms for the retardation effect included the formation of a poor soluble complex between the drug and the additives and the increased viscosity of the medium. A blood level study was also conducted in rats using CTC-HCl and TC-HCl as test materials to determine whether gelatin and acacia affect the membrane permeability of drugs from solutions. Blood concentrations after intraduodenal administration of these two drug solutions with gelatin and acacia were compared to each control level produced by the drug given alone. These two additives resulted in lower blood levels compared to control values.

Photographic binder mixture of three polymers

Abstract: A photographic binder prepared by heating a mixed aqueous solution of styrene-maleic anhydride copolymer, isobutylene-maleic anhydride copolymer and polyvinyl alcohol in acidic state makes it possible to coat a photographic coating liquid for forming photographic layers in the same manner as in the case of using gelatin and furthermore gives excellent physical properties to the resultant photographic layers.

Increasing of photographic coating fluid viscosity

Abstract: PURPOSE:To raise photographic characteristic without gelling gelatine in mixing a coating fluid with gelatin by adding a specified maleic acid copolymer.

Process for obtaining gelatin

Abstract: The invention relates to the manufacture of gelatin, whereby the gelatin is obtained from fresh derms removed from scraps of hides, sinews and tendons within the three hours following the slaughter of the animal; these materials are collected together and washed in cold water then in a solution of dilute sodium hydroxide, subjected to a mechanical separation of fibres until they are reduced to paste, they are then washed several times in cold water in order to eliminate the albumin of the blood, the paste thus washed and rinsed is filtered by pressing until a cake is obtained which is formed of pure collagen; said latter may be stored after dehydration for an indefinite length of time; it may then be subjected to the final cooking, producing a gelatin broth subjected to gelling on a conveyor belt circulating in a chamber maintained at 5° C. and associated with a drying chamber provided with a blower and in which the film of gelatin is conveyed on conveyor belts, the film being wound on a reel on leaving the drying chamber.

Revised production method of substantially linear polyoihalophosphazine solution

Abstract: Dihalophosphazene polymers are solubilized and stabilized whereby their tendency for gelatin or crosslinking is inhibited or overcome, by the addition of oxygen containing organic compounds such as alcohols, ketones, esters, ethers, or polyethers to hydrocarbon solvents for the polymer

Epoxy Resin Embedding in Gelatin Capsules: Improved Orientation of Thin Specimens

Abstract: The hemispherical bottom of ordinary gelatin capsules can be softened by partial soaking in cold water. Pressing the moistened part with a smooth, flatsurfaced object will modify the original shape of the capsule to a flat-bottomed one. Plastic Petri dishes can be used for this work with excellent results. This new shape becomes stable after drying and the capsules thus obtained can be used for orientated embedment of most kinds of samples. Using these modified capsules is particularly helpful for embedding thin specimens such as cells monolayers or fungal mycelia.

Immobilized enzyme and method of preparing the same

Abstract: PURPOSE:Immobilizing enzymes by the use of gelatin as carrier with no reduction in its activity

Low-calorie appetite-satisfying fat or milk foods - contain hollow microspheres having thin pref. crosslinked gelatin walls

Abstract: The food, e.g., a fat- or milk prod., contains hollow microspheres, the thin walls of which consist, at least in part, of gelatin enclosing a single-cell central cavity. The presence of the hollow gelatin microspheres is hardly noticed on chewing soft foods, e.g. spreads on bread. Hollow gelatin microspheres, having dia. 0.2 mm, are softer than microsphere of synthetic plastic. The microspheres form a unit with surrounding food, w.r.t. taste and appearance. The microspheres can enclose up to 50 vol.% air. Gelatin is safely used in foods. The gelatin microspheres resist fats. Water-solubility is reduced by crosslinking, e.g., with HCHO. Crosslinking also increases resistance to digestive juices.

Influence of Physiological Saline, Dextran 70, Hydroxyethyl Starch, Degraded Gelatin, and Fat Emulsion Solutions on Screen Filtration Pressure

Abstract: The effect of 0.9% NaCl solution, dextran 70, hydroxyethyl starch, degraded gelatin and fat emulsion on Hb, Hct, platelet count and screen filtration pressure has been studied in the rabbit. Dextran 70, hydroxyethyl starch and degraded gelatin caused hemodilution and decreased platelet count. Screen filtration pressure increased after infusion of degraded gelatin and tended to decrease after dextran 70 and hydroxyethyl starch. 0.9% NaCl and fat emulsion caused less pronounced changes. Intraportal infusion of 7 ml/kg body weight of the same solutions gave results which at present are difficult to interpret.

Utilization of a fish protein isolate in whippep gelatin desserts

Abstract: A fish protein isolate developed for its whippability was evaluated in a whipped gelatin dessert. The isolate was substituted for 30, 60 or 100% of egg albumin. No significant differences in the desserts were found for appearance, texture, tenderness, mouthfeel, and flavor. Increased specific gravity and rigidity, decreased index of volume and pH accompanied increased levels of the isolate. However, these differences are of relatively little importance in terms of an acceptable product. Panel members indicated a preference for the product with higher levels of the isolate which had a more intense fruit flavor. The isolate can be used as a substitute for egg albumin in production of foams.