Showing papers on "genomic DNA published in 1971"
TL;DR: The chapter presents a picture of the detailed physical structure of the ribosomal cistron and how it is integrated into the genomic DNA, both of which have important implications for cell differentiation and for evolutionary theory.
Abstract: Publisher Summary This chapter reviews that the ribosomal cistrons of both bacteria and eukaryotes, with their unique and well-defined characteristics, have increasingly attracted the attention of biochemists and geneticists. It discusses that the arrangement of the ribosomal cistrons on the chromosomal DNA, and they have been mapped in some detail by means of biophysical techniques. Ribosoinal cistrons were the first Mendelian genes isolated in pure form free from other DNA and it has now become possible to investigate their organization and composition and to study their biological activity in vitro . The chapter focuses on the organizational features and primary structure of DNA. It presents a picture of the detailed physical structure of the ribosomal cistron and how it is integrated into the genomic DNA. It has considered the divergence of ribosomes in different species and the variation of base sequence within the ribosomal cistrons of a single organism, both of which have important implications for cell differentiation and for evolutionary theory.
228 citations
TL;DR: A direct minimum estimate was obtained of the amount of non-repetitive sequence in the oocyte RNA, i.e. its genomic information content, which is 4.5 times the total genomic information present in the Escherichia coli chromosome.
136 citations
TL;DR: It is found that the DNA sequences to which chromosomal RNA binds are repetitive ones, and these sequences are required for sequence-specific interaction of chromosomal proteins with chromosome DNA.
Abstract: Chromosomal RNA, which is a component of the chromosomes of higher organisms, and whose participation is required for sequence-specific interaction of chromosomal proteins with chromosomal DNA, occurs in chromosomes bound to DNA. We have found that the DNA sequences to which chromosomal RNA binds are repetitive ones.
38 citations
TL;DR: Results of pulse-chase experiments indicate that the single-stranded DNA serves as unconserved template and precursor for the synthesis of double-Stranded DNA.
Abstract: The deoxyribonucleic acid (DNA) polymerase of Rous sarcoma virus synthesizes both single- and double-stranded DNA, utilizing the ribonucleic acid (RNA) of the viral genome as the initial template. Results of pulse-chase experiments indicate that the single-stranded DNA serves as unconserved template and precursor for the synthesis of double-stranded DNA. The latter reaction is apparently initiated in association with the viral RNA and may involve a partially double-stranded intermediate form.
21 citations