scispace - formally typeset
Search or ask a question

Showing papers on "genomic DNA published in 1972"


Journal ArticleDOI
Bo Lambert1
TL;DR: Cytological hybridization experiments with RNA from two additional Balbiani rings indicated that the three BR's differ with regard to information content, and that BR-1 like BR-2 contains repeated nucleotide sequences, likely to be involved in the genetic determination of secretory proteins.

56 citations


Journal ArticleDOI
TL;DR: Rous sarcoma virus (RSV)-specific ribonucleic acid (RNA) in virus-producing chicken cells and non-virus-producing rat cells infected with RSV was studied by hybridization with the endogenous deoxyribonuclear acid (DNA) product of the RSV virion DNA polymerase system.
Abstract: Rous sarcoma virus (RSV)-specific ribonucleic acid (RNA) in virus-producing chicken cells and non-virus-producing rat cells infected with RSV was studied by hybridization with the endogenous deoxyribonucleic acid (DNA) product of the RSV virion DNA polymerase system. By hybridizing the total DNA product with excess virion RNA, the product DNA was separated into hybridized (“minus”) and nonhybridized (“plus”) DNA. The “minus” DNA was complementary to at least 20% of the RNA from RSV which remained of high molecular weight after denaturation. A maximum of approximately 65% hybridization was observed between “minus” DNA and RSV RNA or RSV-infected chicken cell RNA. A maximum of about 60% hybridization was observed between “minus” DNA and RSV-infected rat cell RNA. RSV-infected chicken cells contained RSV-specific RNA equivalent to about 6,000 virions per cell. RSV-infected rat cells contained RSV-specific RNA equivalent to approximately 400 virions per cell. Neither cell type contained detectable RNA complementary to virion RNA. The RSV-specific RNA in RSV-infected rat cells did not appear to be qualitatively different from that in RSV-infected chicken cells.

48 citations


Journal ArticleDOI
TL;DR: These experiments now provide the technology required to perform similar examinations of human neoplasias and prove the proof that an RNA-instructed DNA polymerase has been identified.
Abstract: An experimental procedure is detailed that permits the detection of 70S RNA-directed DNA synthesis in mouse mammary carcinomas. The DNA synthesized is complementary to the RNA of the mouse mammary tumor virus by molecular hybridization, thus, completing the proof that an RNA-instructed DNA polymerase has been identified. Further, RNA-instructed DNA polymerase and its 70S RNA template are physically associated in a particle that has a density characteristic of oncornaviruses. These experiments now provide the technology required to perform similar examinations of human neoplasias.

43 citations


Journal ArticleDOI
Keiji Ohe1
01 Mar 1972-Virology
TL;DR: After infection with adenoviruses, KB cells synthesize a unique species of low molecular weight RNA (VA RNA), which is coded by the infecting viral genome.

27 citations


Book ChapterDOI
TL;DR: The study of the kinetics of DNA renaturation has yielded much information about the organization of the DNA in eukaryotes into families of nucleotide sequences, and it has been shown that the 800-fold redundant ribosomal RNA genes of Xenopus laevis form a family of closely related base sequence.
Abstract: The study of the kinetics of DNA renaturation has yielded much information about the organization of the DNA in eukaryotes into families of nucleotide sequences (Britten and Kohne, 1967, 1968; Wetmur and Davidson, 1968). With a few exceptions the entire assemblage of families of related base sequences of any one organism is treated as a single body of DNA. It is possible to study the renaturation behaviour of a family of genes coding for a specific RNA of genes within the total genomic DNA, but such experiments are tedious to carry out and so fax have required a combination of hydroxylapatite fractionation of the renaturing DNA (Flamm et al., 1969) and subsequent identification of the specific DNA segments by RNA/DNA hybridization. By this indirect approach it has been shown, for example, that the 800-fold redundant ribosomal RNA genes of Xenopus laevis form a family of closely related base sequence (Grunstein, unpublished) Similarly, after preselection of ribosomal DNA complements from bacteria by RNA/DNA hybridization the renaturation kinetics for these DNA segments indicate that bacterial ribosomal cistrons are similar in base sequence (Kohne, 1968).

6 citations