Topic
genomic DNA
About: genomic DNA is a research topic. Over the lifetime, 15046 publications have been published within this topic receiving 663636 citations. The topic is also known as: genomic deoxyribonucleic acid & gDNA.
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TL;DR: The nucleotide sequence of the major subgenomic RNA of the calicivirus rabbit hemorrhagic disease virus which codes for the viral capsid protein has been cloned as cDNA was shown to be identical to the 3′ terminal region of the genomic RNA.
183 citations
Journal Article•
TL;DR: The observations indicate that this DNA sequence, although naturally carried by a bacterial plasmid, is programmed as a functional plant gene.
Abstract: We have determined the complete nucleotide sequence of the gene for the crown gall enzyme, octopine synthase The sequence was derived from cloned fragments of the Agrobacterium tumefaciens Ti plasmid Ach5 It displayed a continuous open reading frame encoding a polypeptide chain of 358 amino acids The nucleotide positions corresponding to the 5' end and poly(A) addition site of the mature octopine synthase mRNA from a tobacco tumor cell line were determined by S1 nuclease mapping Two sequences closely resembling transcriptional control regions found in eukaryotic genes transcribed by RNA polymerase II were identified in the flanking genomic DNA: a sequence 5'-TATTTAAA-3' was located 32 base pairs upstream from the initiation site of transcription, and a hexanucleotide 5'-AATAAT-3' occurred 17 base pairs in front of the poly(A) addition site No Shine-Dalgarno sequence was present in the untranslated 5' leader sequence The observations indicate that this DNA sequence, although naturally carried by a bacterial plasmid, is programmed as a functional plant gene
183 citations
TL;DR: It is demonstrated that a current ABE generates low but detectable levels of widespread adenosine-to-inosine editing in cellular RNAs, as well as lower off-target DNA editing activity and reduced indel by-product formation, in three mammalian cell lines.
Abstract: Adenine base editors (ABEs) enable precise and efficient conversion of target A•T base pairs to G•C base pairs in genomic DNA with a minimum of by-products. While ABEs have been reported to exhibit minimal off-target DNA editing, off-target editing of cellular RNA by ABEs has not been examined in depth. Here, we demonstrate that a current ABE generates low but detectable levels of widespread adenosine-to-inosine editing in cellular RNAs. Using structure-guided principles to design mutations in both deaminase domains, we developed new ABE variants that retain their ability to edit DNA efficiently but show greatly reduced RNA editing activity, as well as lower off-target DNA editing activity and reduced indel by-product formation, in three mammalian cell lines. By decoupling DNA and RNA editing activities, these ABE variants increase the precision of adenine base editing by minimizing both RNA and DNA off-target editing activity.
183 citations
TL;DR: These results demonstrate, for the first time, the possibility of targeting episomal viral DNA genomes using ZFNs and decrease levels of the hepatitis C virus pregenomic RNA by 29%.
181 citations
TL;DR: The entire human pepsinogen C gene has been isolated from a cosmid genomic library and the nucleotide sequences of all the exons and the 5'- and 3'-flanking regions of the gene are presented, allowing us to conclude that the genes of these aspartic proteinases including peps inogen C are derived from a common ancestral gene.
181 citations