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genomic DNA

About: genomic DNA is a research topic. Over the lifetime, 15046 publications have been published within this topic receiving 663636 citations. The topic is also known as: genomic deoxyribonucleic acid & gDNA.


Papers
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Journal ArticleDOI
TL;DR: An unusually long repeated DNA sequence was identified in cloned DNA, three kb 3' to the human beta-globin gene, which does not appear to be composed of any smaller tandemly repeated subunits, nor is it expressed at a high level in bone marrow cell RNA.
Abstract: An unusually long repeated DNA sequence was identified in cloned DNA, three kb 3' to the human beta-globin gene. Other members of this repeated sequence family were isolated from a human genomic DNA library and characterized by Southern blotting techniques, electron microscopy, and solution hybridization. The copy located next to the beta-globin gene was found to be 6.4 +/- 0.2 kb long and continuous over that length. This repeated sequence family comprises about 1% of the human genome and contains 3000-4800 copies of moderate sequence divergence which are interspersed with other less-highly repeated DNA. The 6.4 kb repeated unit does not appear to be composed of any smaller tandemly repeated subunits, nor is it expressed at a high level in bone marrow cell RNA.

127 citations

Journal ArticleDOI
TL;DR: Domain L was found to be rich in basic amino acid residues, especially for exon 3, and its N-terminal half was highly conserved among species, and the multiple isoforms are implicated for specific physiological roles.

127 citations

Journal Article
TL;DR: DNA walking on plant genomic DNA is an extremely efficient alternative to inverse PCR for the characterization of T-DNA flanking regions and eliminates the requirement of screening libraries for the isolation of genomic regions flanking coding sequences, ESTs, STSs or any short, known sequence.

127 citations

Journal ArticleDOI
TL;DR: A novel member of the family of G protein‐coupled receptors has been isolated from a mouse brain cDNA library by screening with polymerase chain reaction (PCR) generated fragment of mouse genomic DNA amplified using degenerated primers, demonstrating that the encoded protein sequence shows the highest homology to the 5‐HT2 family of receptors.

127 citations

Journal ArticleDOI
TL;DR: Specific DNA restriction endonuclease fragments can be identified after electrophoresis in agarose gels by hybridization in the gel (in situ) to radioactive homologous RNA by comparison of band patterns of the autoradiogram and the ethidium bromide stained gel.
Abstract: Specific DNA restriction endonuclease fragments can be identified after electrophoresis in agarose gels by hybridization in the gel (in situ) to radioactive homologous RNA. RNA-DNA hybrids are detected by autoradiography of the gel. Comparison of band patterns of the autoradiogram and the ethidium bromide stained gel allows the identification of the DNA fragment which is complementary to the RNA probe. The technique is rapid, easy and inexpensive. It is sensitive enough to detect individual genes in a mixture of fragments produced by restriction enzyme digestion of complex cellular DNA. We have used this technique to determine which of the Hin III and Eco R1 fragments of phi80d3ilv+su+7 and E. coli DNAs contain the 5S, 16S and 23S ribosomal RNA (rRNA) genes of E. coli.

127 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023258
2022431
2021232
2020261
2019273
2018339