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genomic DNA

About: genomic DNA is a research topic. Over the lifetime, 15046 publications have been published within this topic receiving 663636 citations. The topic is also known as: genomic deoxyribonucleic acid & gDNA.


Papers
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Journal ArticleDOI
TL;DR: The cloning and characterization of human and mouse genes for VEGF-C, a newly isolated member of the vascular endothelial growth factor/platelet-derived growth factor (VEGF/PDGF) family, shows both similarities and distinct differences in comparison with other members of the V EGF/ PDGF gene family.

214 citations

Journal ArticleDOI
01 Mar 1982-Cell
TL;DR: The results suggest that specific double-stranded DNA sequences are recognized by the oviduct progesterone-receptor complex in vitro, and are relevant to the question of whether specificDNA sequences are directly involved as genomic binding sites for steroid receptors.

212 citations

Journal ArticleDOI
TL;DR: The authors have isolated human genomic DNA clones encompassing 30 kbp of the histo-blood group ABO locus and the locations of the exons have been mapped and the nucleotide sequences of theExon-intron boundaries have been determined.
Abstract: We have isolated human genomic DNA clones encompassing 30 kbp of the histo-blood group ABO locus. The locations of the exons have been mapped and the nucleotide sequences of the exon-intron boundaries have been determined. The human ABO genes consist of at least seven exons, and the coding sequence in the seven coding exons spans over 18 kb of the genomic DNA. The exons range in size from 28 to 688 bp, with most of the coding sequence lying in exon 7.

212 citations

Journal ArticleDOI
TL;DR: Real-time 5' nuclease PCR assays to rapidly distinguish single-base polymorphism using a battery-powered miniature analytical thermal cycling instrument (MATCI) to demonstrate the feasibility of using the MATCI for diagnosis of infectious diseases and genetic disorders.
Abstract: This report describes real-time 5' nuclease PCR assays to rapidly distinguish single-base polymorphism using a battery-powered miniature analytical thermal cycling instrument (MATCI). Orthopoxviruses and the human complement component C6 gene served as targets to demonstrate the feasibility of using the MATCI for diagnosis of infectious diseases and genetic disorders. In the Orthopoxvirus assay, consensus Orthopoxvirus PCR primers were designed to amplify 266-281 base-pair (bp) segments of the hemagglutinin (HA) gene in camelpox, cowpox, monkeypox, and vaccinia viruses. A vaccinia virus-specific fluorogenic (TaqMan) probe was designed to detect a single-base (A/G) substitution within the HA gene. In the C6 gene assay, a 73-bp segment of the C6 gene was PCR-amplified from human genomic DNA, and TaqMan probes were used to detect a single-base (A/C) polymorphism in the second position of codon 98. The MATCI correctly identified the nucleotide differences in both viral DNA and human genomic DNA. In addition, using a rapid DNA preparation method, it was possible to achieve sample, preparation of human genomic DNA, DNA amplification, and real-time detection in less than 1 h.

211 citations

Journal ArticleDOI
TL;DR: DNA prepared by this new method is an excellent substrate for restriction endonuclease digestion and Southern hybridization analysis and sodium perchlorate and chloroform instead of phenol with a significant time savings realized as well as fewer hazards to the technician.

211 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023258
2022431
2021232
2020261
2019273
2018339