Topic
Genomic library
About: Genomic library is a research topic. Over the lifetime, 7977 publications have been published within this topic receiving 481143 citations.
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TL;DR: The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
Abstract: Unique DNA sequences can be determined directly from mouse genomic DNA. A denaturing gel separates by size mixtures of unlabeled DNA fragments from complete restriction and partial chemical cleavages of the entire genome. These lanes of DNA are transferred and UV-crosslinked to nylon membranes. Hybridization with a short 32P-labeled single-stranded probe produces the image of a DNA sequence "ladder" extending from the 3' or 5' end of one restriction site in the genome. Numerous different sequences can be obtained from a single membrane by reprobing. Each band in these sequences represents 3 fg of DNA complementary to the probe. Sequence data from mouse immunoglobulin heavy chain genes from several cell types are presented. The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
7,858 citations
Book•
01 Jan 1986
TL;DR: General methods bacterial strains and cloning vectors enzymes that modify DNA and RNA in vitro amplification of DNA using the polymerase chain reaction (PCR) and the thermostable Taq DNA polymerase, introduction DNA restriction fragment analysis and preparation.
Abstract: General methods bacterial strains and cloning vectors enzymes that modify DNA and RNA in vitro amplification of DNA using - the polymerase chain reaction (PCR) and the thermostable Taq DNA polymerase, introduction DNA restriction fragment analysis and preparation, introduction in vitro labeling of probes and filter hybridization plasmid DNA preparation for E. Coli hosts preparation of DNA from Lambda bacteriophage clones subcloning fragments into plasmid vectors and plasmid construction preparation of genomic DNA preparation and analysis of RNA from eukaryotic cells - overview geonomic cloning generatino of cDNA libaries preparation of subtractive cDNA, introduction chain termination sequencing transfection of mammalian cells, introduction basic methods of protein analysis in situ hybridization transgenic mouse preparation detection and in vitro generation of specific mutations in genes and cDNAs, introduction appendices.
4,526 citations
TL;DR: A method is described for the rapid generation and cloning of deletion derivatives well-suited for the sequencing of long stretches of DNA based on two useful features of exonuclease III: processive digestion at a very uniform rate and failure to initiate digestion at DNA ends with four-base 3'-protrusions.
3,849 citations
TL;DR: An approach to DNA sequencing using chain-terminating inhibitors (Sanger et al., 1977) combined with cloning of small fragments of DNA in a single-stranded DNA bacteriophage is described, determining the 2771-nucleotide sequence of the largest MboI restriction enzyme fragment from human mitochondrial DNA.
2,902 citations
TL;DR: The results suggest that a single large phage display library can be used to isolate human antibodies against any antigen, by-passing both hybridoma technology and immunization.
2,678 citations