Topic
Gibberellic acid
About: Gibberellic acid is a research topic. Over the lifetime, 6597 publications have been published within this topic receiving 109294 citations. The topic is also known as: GIBBERELLIN A3.
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TL;DR: The role of endosymbiont Piriformospora indica in counteracting salinity stress to Pakchoi plants was determined by studying physiological, biochemical, and molecular mechanisms.
35 citations
TL;DR: The xyloglucan fragment was found to inhibit gibberellic acid-induced elongation of etiolated pea epicotyls with maximum inhibition at concentrations ranging from 10 -11 to 10 -9 M.
Abstract: The biological activity of a cell wall-derived xyloglucan nonasaccharide (XG9) was investigated using a bioassay with entire pea epicotyls (Pisum sativum cv. Progress). The xyloglucan fragment was found to inhibit gibberellic acid-induced elongation of etiolated pea epicotyls with maximum inhibition at concentrations ranging from 10 -11 to 10 -9 M. Growth of etiolated epicotyls in the absence of exogenously applied GA 3 was also inhibited by XG9 in the same concentration range
35 citations
TL;DR: Results indicate that gibberellin A3, applied during the induction as well as the differentiation stage, reduced the weight of callus and increased the number ofomatic embryos in Medicago sativa L tissue cultures.
Abstract: The influence of exogenous gibberellic acid (GA3) andpaclobutrazol, an inhibitor of gibberellin biosynthesis, on growth of callusandsomatic embryogenesis in petiole-derived tissue cultures of Medicagosativa L. has been investigated. GA3 (0.5–500μM) or paclobutrazol(5–100 μM) were added to either an induction (with 2,4 Dand kinetin) or a differentiation medium (without plant growth regulators).Gibberellin A3, applied during the induction as well as thedifferentiation stage, reduced the weight of callus and increased the number ofsomatic embryos in Medicago sativa L. tissue cultures.Somatic embryo production was increased more by the presence of exogenousGA3 in the differentiation than induction medium. The inclusion ofpaclobutrazol in the induction or differentiation medium caused the inhibitionof callus growth and embryo production. Callus growth was much less affectedthan embryogenesis. These results indicate that gibberellins are beneficial forboth embryoinduction and formation. The level of endogenous gibberellins is presumablysufficient for callus induction and growth. However, it seems not optimal forthe induction and particularly for the differentiation of embryos.
35 citations
TL;DR: In excised Avena leaves, kinetin and benzyladenine decreased, while abscisic acid and benzimidazole increased the over-all nuclease level, and the level of an alkaline phosphodiesterase, an exo-nuclease, was not affected by any of the growth regulators tested.
Abstract: In excised Avena leaves, kinetin and benzyladenine decreased, while abscisic acid and benzimidazole increased the over-all nuclease level. Significant effects were observed as early as 2 h after treatment. Not all the nucleases of the Avena leaf were affected by the growth regulators. Changes in over-all nuclease activity were accounted for almost entirely by changes in the amount of a relatively purine-specific endo-ribonuclease, which produces 2', 3'-cyclic phosphates as breakdown products. Slight changes induced by the growth regulators were also detected in the amount of a sugar non-specific endo-nuclease which produces 5'-nucleotides and has a relative specificity for adenylic acid. The level of an alkaline phosphodiesterase, an exo-nuclease which produces 5'-nucleotides, was not affected by any of the growth regulators tested. Gibberellic acid and indol-3yl-acetic acid did not influence the level of Avena nucleases.
35 citations
TL;DR: Callus cultures of Datura innoxia grew rapidly (100-fold in 4 weeks) on a slightly modified Murashige and Skoog medium and did not grow significantly better when BAP was combined with one of the auxins or with GA.
Abstract: Datura innoxia Mill. callus cultures formed shoots in 2–4 weeks on media containing; a) gibberellic acid, b) indoleacetic acid, c) low concentrations of naphthylacetic acid, d) low concentrations of 2,4-dichlorophenoxyacetic acid, e) benzylaminopurine, f) no growth substance.
Benzylaminopurine promoted shoot differentiation. Gibberellic acid inhibited shoot formation weakly, but inhibited proper leaf blade formation.
Root differentiation was rare.
The callus cultures of Datura innoxia grew rapidly (100-fold in 4 weeks) on a slightly modified Murashige and Skoog medium (0.5 mg/l thiamin · HCl, pH 5.5, no glycine) in light at 30°C. Callus grew well on any single one of the growth substances NAA (10−5M), 2,4-D (10−6M) or BAP (3 × 10−6M). Growth was less and more erratic on GA or IAA. The callus cultures did not grow significantly better when BAP was combined with one of the auxins or with GA.
35 citations