Topic
Gibberellic acid
About: Gibberellic acid is a research topic. Over the lifetime, 6597 publications have been published within this topic receiving 109294 citations. The topic is also known as: GIBBERELLIN A3.
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TL;DR: For interspecific hybrids, a 5-day pre-incubation of the embryos on a liquid formulation was better than the solid formulation as it promoted better growth and vigour of the normally abortive interspecific hybrid embryos.
Abstract: An improved embryo-rescue protocol was developed for embryos (90 days old) of Carica papaya L. (Clone 2001), and subsequently was utilised for efficient production of interspecific hybrids of C. papaya × C. cauliflora Jacq. from 90- to 120-day-old embryos. Pre-incubation of C. papaya embryos for 7 days on a germination medium containing half-strength De Fossard nutrients supplemented with gibberellic acid (10 μM), 6-benzylamino-purine (0.25 μM), alpha-naphthalene-acetic acid (0.25 μM). sucrose (58 mM) and agar (8 g L-1) supported 100% germination. Subsequent transfer of germinated embryos to a nutrient medium that was identical, except that it was free of plant growth regulator, allowed good growth but induced shoot etiolation and callus production. Reducing the pre-incubation of C. papaya embryos on this medium to 5 days before transfer to the medium free of plant growth regulator produced similarly high germination (96%), but allowed for the production of good quality seedlings that were unetiolated and free of unwanted callus. For interspecific hybrids, a 5-day pre-incubation of the embryos on a liquid formulation was better than the solid formulation as it promoted better growth and vigour of the normally abortive interspecific hybrid embryos. Using the improved protocol, 1981 of 2100 (94%) interspecific hybrid embryos consisting of single and multiple forms were germinated. In all cases, the germinating multiple embryos underwent further embryogenesis that allowed for the production of 485 (25%) morphologically normal hybrid plants grown in soil in the glasshouse.
33 citations
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TL;DR: Gibberellic acid antagonism of the growth inhibitory and stress protective effects of paclobutrazol was investigated in wheat seedlings and changes in protein expression were investigated in an attempt to understand the basis of P-induced stress protection.
32 citations
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TL;DR: The regenerated plants were generally shorter and less vigorous than the original donor material, but the majority of those examined had the expected euploid chromosome number indicating that their altered morphology had a physiological rather than a genetic basis.
32 citations
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TL;DR: The formation of gibberellic acid in single‐stage continuous cultures was studied and the rate of formation, calculated on the basis of mycelial nitrogen, was comparable with the highest values reported for batch cultures.
Abstract: The formation of gibberellic acid in single-stage continuous cultures was studied. Stirred fermenters of 3 and 20-l. working volumes were used. Maximum output of gibberellic acid was obtained at a holding time of 200 hr. The rate of formation, calculated on the basis of mycelial nitrogen, was comparable with the highest values reported for batch cultures.
32 citations