Topic
Gibberellic acid
About: Gibberellic acid is a research topic. Over the lifetime, 6597 publications have been published within this topic receiving 109294 citations. The topic is also known as: GIBBERELLIN A3.
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TL;DR: Pulse-labeling of barley and Immunoprecipitation of translation products showed that the RNA for α-amylase synthesized in Ca2+-deprived aleurone layers was translatable.
Abstract: Pulse-labeling of barley (Hordeum vulgare L. cv Himalaya) aleurone layers incubated for 13 hours in 2.5 micromolar gibberellic acid (GA3) with or without 5 millimolar CaCl2 shows that α-amylase isozymes 3 and 4 are not synthesized in vivo in the absence of Ca2+. A cDNA clone for α-amylase was isolated and used to measure α-amylase mRNA levels in aleurone layers incubated in the presence and absence of Ca2+. No difference was observed in α-amylase mRNA levels between layers incubated for 12 hours in 2.5 micromolar GA3 with 5 millimolar CaCl2 and layers incubated in GA3 alone. RNA isolated from layers incubated for 12 hours in GA3 with and without Ca2+ was translated in vitro and was found to produce the same complement of translation products regardless of the presence of Ca2+ in the incubation medium. Immunoprecipitation of translation products showed that the RNA for α-amylase synthesized in Ca2+-deprived aleurone layers was translatable. Ca2+ is required for the synthesis of α-amylase isozymes 3 and 4 at a step after mRNA accumulation and processing.
72 citations
Journal Article•
TL;DR: Irrespective of its concentration, spray of gibberellic acid proved beneficial for most parameters, especially in the case of Hyb-SC-3.
Abstract: A pot experiment was performed according to a factorial randomized design at Aligarh to study the effect of 4 levels of gibberellic acid spray (0, 10 -8 , 10 -6 and 10 -4 M GA3) on the growth, leaf-NPK content, yield and quality parameters of 2 tomato cultivars (Lycopersicon esculentum Mill.), namely Hyb-SC-3 and Hyb-Himalata. Irrespective of its concentration, spray of gibberellic acid proved beneficial for most parameters, especially in the case of Hyb-SC-3.
72 citations
TL;DR: The maturation of embryos was significantly affected by pH, photoperiod, abscisic acid and genotype, and all regenerated plants showed normal morphological characteristics.
Abstract: Somatic embryogenesis and plant regeneration were obtained from immature leaflet callus of chickpea. Numerous globular embryos developed on the surface of callus on Murashige and Skoog's (1962) medium containing 25 μM 2,4-dichlorophenoxyacetic acid. These globular embryos differentiated into mature somatic embryos upon removal of 2,4-dichlorophenoxyacetic acid. The maturation of embryos was significantly affected by pH, photoperiod, abscisic acid and genotype. Callus continued to produce somatic embryos for over 8 subcultures at 4 week intervals. Two per cent of the embryos formed plants on medium containing 15 μM gibberellic acid and 1 μM indole-3-butyric acid. Desiccation of embryos for a period of 3 d increased their rate of conversion into plants from 0.9 to 2.8%. All regenerated plants showed normal morphological characteristics.
72 citations
TL;DR: It is shown here that for one of those cDNA clones from mRNAs which are produced in increased amounts when aleurone layers of wheat are treated with gibberellic acid, the change in level of mRNA in aleur one parallels the changeIn level of alpha-amylase mRNA.
72 citations
TL;DR: Abscisic acid inhibits stomatal opening in isolated abaxial epidermal strips of Vicia faba and appears to act directly on the guard cells, rather than by influencing water potentials throughout the leaf.
Abstract: Abscisic acid inhibits stomatal opening in isolated abaxial epidermal strips of Vicia faba. Kinetin, benzyladenine, and gibberellic acid, which can enhance stomatal apertures in intact leaves, are ...
72 citations