Topic
Gibberellic acid
About: Gibberellic acid is a research topic. Over the lifetime, 6597 publications have been published within this topic receiving 109294 citations. The topic is also known as: GIBBERELLIN A3.
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TL;DR: From a comparison of the effects of seven growth retardants and abscisic acid (ABA) on various growth systems, it was found that the gibberellin-regulated growth of lettuce hypocotyls was uniquely inhibited by the growth retardant, alpha-cyclopropyl-alpha-(4-methoxyphenyl)-5-pyrimidine methanol (EL-531).
Abstract: From a comparison of the effects of seven growth retardants and abscisic acid (ABA) on various growth systems, it was found that the gibberellin-regulated growth of lettuce hypocotyls was uniquely inhibited by the growth retardant, alpha-cyclopropyl-alpha-(4-methoxyphenyl)-5-pyrimidine methanol (EL-531). Auxin-regulated growth of coleoptile sections was inhibited by Phosfon and only slightly by EL-531 and Alar. Cytokinin-regulated growth of Xanthium cotyledons showed little or no inhibition by any of the retardants. ABA was inhibitory in all three types of tests. The distinctive effects of EL-531 against gibberellin-stimulated growth and the general ability of gibberellic acid to relieve EL-531 inhibition suggest that this retardant acts in part against the gibberellin-stimulated growth system, but at a locus which discriminates between growth and nongrowth functions of gibberellic acid. It shows little or no antagonism of gibberellin actions which do not involve growth: the barley endosperm test and the Rumex leaf senescence test.
59 citations
59 citations
TL;DR: In-depth root-specific gene expression analyses carried out in the wild type and the lateral root mutants lrt1 and rum1 suggest a role for ZmGSL genes in early lateral root development, which is likely regulated by gibberellic acid.
Abstract: In an approach to study lateral root development in monocots, genome-wide searches for homologs of the Gibberellic Acid Stimulated Transcript-like (GAST-like) gene family in rice (Oryza sativa) and maize (Zea mays) were carried out. Six novel GAST-like genes in rice and 10 members of the gene family in maize, which were designated ZmGSL (for Z. mays Gibberellic Acid Stimulated-Like), were identified. The ZmGSL family encodes small proteins of 75 to 128 amino acids, which are characterized by a conserved 59 to 64 amino acid C-terminal domain. Within this domain, 17 amino acids, including 12 cysteines, are perfectly conserved. The transcript of the ZmGSL1 gene is differentially spliced into the alternative variants ZmGSL1a and ZmGSL1b, the latter of which is translated into a premature protein that lacks the C-terminal domain. The presence of an additional N-terminal cleavable signal sequence in eight of the 10 ZmGSL proteins suggests that they are secreted into the extracellular matrix. In-depth root-specific gene expression analyses carried out in the wild type and the lateral root mutants lrt1 and rum1 suggest a role for ZmGSL genes in early lateral root development, which is likely regulated by gibberellic acid. Expression patterns of ZmGSL1a and ZmGSL1b propose antagonistic functions of these splice variants during early lateral root formation.
58 citations
TL;DR: Clonal micropropagation was established by shoot tip culture and was maintained on Murashige and Skoog medium supplemented with kinetin and gibberellic acid A 3 and callus that was induced and maintained on supplements of 2,4-dichlorophenoxyacetic acid and 6-benzylaminoputine in the absence of light.
Abstract: Callus tissue from petioles of Salvia miltiorrhiza was obtained on Murashige and Skoog medium supplemented with indole-3-butyric acid and 6-benzylaminopurine. When the calli were subcultured, adventitious shoots formed. These shoots developed into normal plantlets with roots when transferred to hormone-free Murashige and Skoog medium. Clonal micropropagation was established by shoot tip culture and was maintained on Murashige and Skoog medium supplemented with kinetin and gibberellic acid A 3 . Callus that was induced and maintained on supplements of 2,4-dichlorophenoxyacetic acid and 6-benzylaminoputine in the absence of light produced rosmarnic acid (1.24% dry wt) and lithospermic acid B (0.10% dry wt)
58 citations
TL;DR: It was found that ascorbate, at levels of 4−8×10-4M, enhanced shoot formation in both young and old callus, and treatment with ascorBate speeded up the shoot-forming process.
Abstract: The effect of ascorbic acid on growth and shoot formation in callus cultures of tobacco (Nicotiana tabacum L.) was investigated, using young (4–12 subcultures) and old (more than 30 subcultures) tissue. It was found that ascorbate, at levels of 4−8×10-4M, enhanced shoot formation in both young and old callus. Treatment with ascorbate also speeded up the shoot-forming process. In addition, ascorbate completely reversed the inhibition of shoot formation by gibberellic acid in young callus, but was less effective in old callus.
58 citations