Topic
Glutaraldehyde
About: Glutaraldehyde is a research topic. Over the lifetime, 4818 publications have been published within this topic receiving 153274 citations. The topic is also known as: Glutaric dialdehyde & 1,5-Pentanedial.
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TL;DR: Aldehydes are the most commonly used fixative for microscopy and serve to stabilize the fine structural details of cells and tissues prior to examination by light or electron microscopy.
Abstract: Aldehydes are the most commonly used fixatives. They serve to stabilize the fine structural details of cells and tissues prior to examination by light or electron microscopy. Research workers, technicians, pathologists and others who regularly use aldehyde fixatives frequently do not appreciate the nature and properties of these compounds or the reasons for choosing to fix a specimen in formaldehyde, glutaraldehyde or a mixture of the two. Misconceptions are widespread also about formalin and paraformaldehyde, the commercial products from which formaldehyde-containing solutions are made.
440 citations
TL;DR: In this paper, the development and characterization of novel polymer blends based on chitosan and polyvinyl alcohol and chemically crosslinked by glutaraldehyde for possible use in a variety of biomedical applications is reported.
432 citations
TL;DR: Poly(carboxylic acids) that can crosslink in both dry and wet conditions have been shown to provide the desired improvements in tensile properties, increase in stability under aqueous conditions, and also promote cell attachment and proliferation.
430 citations
TL;DR: This work enhances crosslinking through bridging of activated carboxyl groups with diamines and using glutaraldehyde to crosslink the epsilon-NH2 groups in collagen and the unreacted amines introduced by aliphatic diamines, which reduces tissue degradation and nearly eliminates humoral antibody induction.
Abstract: Glutaraldehyde crosslinking of native or reconstituted collagen fibrils and tissues rich in collagen significantly reduces biodegradation. Other aldehydes are less efficient than glutaraldehyde in generating chemically, biologically, and thermally stable crosslinks. Tissues crosslinked with glutaraldehyde retain many of the viscoelastic properties of the native collagen fibrillar network which render them suitable for bioprostheses. Implants of collagenous materials crosslinked with glutaraldehyde are subject long-term to calcification, biodegradation, and low-grade immune reactions. We have attempted to overcome these problems by enhancing crosslinking through bridging of activated carboxyl groups with diamines and using glutaraldehyde to crosslink the epsilon-NH2 groups in collagen and the unreacted amines introduced by aliphatic diamines. This crosslinking reduces tissue degradation and nearly eliminates humoral antibody induction. Covalent binding of diphosphonates, specifically 3-amino-1-hydroxypropane-1, 1-diphosphonic acid (3-APD), and chondroitin sulfate to collagen or to the crosslink-enhanced collagen network reduces its potential for calcification. Platelet aggregation is also reduced by glutaraldehyde crosslinking and nearly eliminated by the covalent binding of chondroitin sulfate to collagen. The cytotoxicity of residual glutaraldehyde--leaching through the interstices of the collagen fibrils or the tissue matrix--and of reactive aldehydes associated with the bound polymeric glutaraldehyde can be minimized by neutralization and thorough rinsing after crosslinking and storage in a nontoxic bacteriostatic solution.
413 citations
TL;DR: In this paper, the authors compared Glutaraldehyde, formaldehyde, and osmium tetroxide perfusion fixation of the central nervous system of rats and found that a very close cell-to-cell membrane relationship is universally found, restricting the extracellular space to considerably less than that previously reported.
412 citations