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Growth medium

About: Growth medium is a research topic. Over the lifetime, 1889 publications have been published within this topic receiving 59171 citations. The topic is also known as: culture medium & culture media.


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Journal ArticleDOI
TL;DR: Growth in high salt likely leads to reduced phosphate uptake in A. doliolum, and NaCl effects on protein and chlorophyll concentrations were greater in P-deficient medium, while presence of P in the medium had little effect on cellular carbohydrate concentrations.
Abstract: In the present study, we have investigated the effects of NaCl concentrations on the growth and phosphate metabolism of an Anabaena doliolum strain isolated from a paddy field, in order to determine the possible effects of salinization. Growth rate, chlorophyll content, and protein content decreased with increasing salt concentration in the growth medium, while carbohydrate concentration increased. Phosphate content and phosphate uptake rate decreased. There was an increase in total alkaline phosphatase activity, with an approximately 7-fold increase in extracellular activity compensating for an approximately 3-fold decrease in cell-bound activity. NaCl effects on protein and chlorophyll concentrations were greater in P-deficient medium, while presence or absence of P in the medium had little effect on cellular carbohydrate concentrations. It is concluded that growth in high salt likely leads to reduced phosphate uptake in A. doliolum.

32 citations

Journal ArticleDOI
TL;DR: Results suggest that the detection of C. fetus subsp.
Abstract: The combination of medium and growth conditions, including transport enrichment medium (TEM), transport time, TEM incubation time, and growth medium, that best support Campylobacter fetus subsp. venerealis while inhibiting contaminants was studied. The 3 TEMs evaluated, Weybridge, Cary-Blair, and 0.85% saline solution, were inoculated with preputial smegma spiked with C. fetus subsp. venerealis and held in the laboratory for 4 or 24 hours before inoculation onto growth medium. The effect of overnight incubation at 37 C of the TEM was also evaluated. Median scores of C. fetus subsp. venerealis growth and microbial contaminant inhibition were compared within TEM, transport time, overnight incubation, and growth medium groups using the Mann-Whitney U-test and the Kruskal-Wallis test. The proportion of samples with any growth or contamination within each group was also compared using the chi-square test. Results suggest that the growth of C. fetus subsp. venerealis was influenced by 3 of the 4 criteria evaluated. Weybridge TEM more effectively maintained the organism than did either Cary-Blair or 0.85% saline solution (P < 0.001). Transport time of 4 hours rather than 24 hours (P < 0.001) and avoiding overnight incubation of TEM at 37 C (P < 0.001) were associated with improved growth. Significant differences were not identified among growth media; however, Skirrow Campylobacter agar appeared to yield slightly better growth than did either blood agar or Greenbriar Plus agar. Contaminant growth was also influenced by 3 of the 4 variables. Weybridge TEM inhibited contaminant growth more effectively than did either Cary-Blair or 0.85% saline solution (P < 0.001). Transport time was not associated with contaminant growth. Eliminating overnight incubation of TEM reduced contamination (P < 0.01). Skirrow agar was preferred to both blood agar and Greenbriar Plus agar for suppression of contaminants on solid medium (P < 0.001). These results suggest that the detection of C. fetus subsp. venerealis is enhanced when preputial smegma samples arrive at the diagnostic laboratory within 4 hours after inoculation into Weybridge TEM and are transferred to Skirrow agar the same day they arrive in the laboratory.

32 citations

Patent
05 May 2009
TL;DR: In this article, a reduced general control response to amino acid starvation was found to have increased tolerance to butanol in the growth medium and the reduced response was engineered by genetic modification of a gene involved in the response, a GCN gene.
Abstract: Yeast cells with a reduced general control response to amino acid starvation were found to have increased tolerance to butanol in the growth medium. The reduced response was engineered by genetic modification of a gene involved in the response, a GCN gene, to eliminate activity of the encoded protein. Yeast strains with an engineered butanol biosynthetic pathway and a genetic modification in a gene involved in the general control response to amino acid starvation, which have increased butanol tolerance, are useful for production of butanol.

32 citations

Journal ArticleDOI
TL;DR: Analysis of the metabolism of radiolabelled mannose and sucrose in the presence and absence of glucose demonstrated that glucose functions as the preferred carbon source, inhibiting the uptake and oxidation of the sugars within 15 min of its addition.
Abstract: The formation of aerial mycelia and spores by Streptomyces alboniger has been observed to be inhibited by glucose supplied in the growth medium as the sole carbon source or supplied in combination with other utilizable carbon sources. Analysis of the metabolism of radiolabelled mannose and sucrose in the presence and absence of glucose demonstrated that glucose functions as the preferred carbon source, inhibiting the uptake and oxidation of the sugars within 15 min of its addition. The inhibition of aerial mycelium formation was shown to result from the excretion of an acidic metabolite, and could be overcome by the addition of a buffering system. The acid metabolite was identified as pyruvic acid by high-performance liquid chromatography and by paper chromatography. Acid was not produced in substantial quantities in dextrin broth or in glucose broth supplemented with 5 mM adenine. Analysis of the pathway of pyruvate overproduction demonstrated that growth on glucose resulted in increased glycolytic activ...

32 citations

Journal ArticleDOI
TL;DR: The proteolytic activity of Lactobacillus bulgaricus LBB grown in skim milk was determined at 42°C as a function of growth temperature and TCA-soluble N in the growth medium as discussed by the authors.

32 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20233
20226
202126
202032
201926
201829