Topic
Growth medium
About: Growth medium is a research topic. Over the lifetime, 1889 publications have been published within this topic receiving 59171 citations. The topic is also known as: culture medium & culture media.
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TL;DR: A variety of mammalian cells growing in tissue culture took up appreciable amounts of lipid from the serum used in the medium, and for the MB III strain of mouse lymphoblasts, triglycerides were most rapidly utilized followed by phospholipids and thea cholesterol.
Abstract: Summary1) A variety of mammalian cells growing in tissue culture took up appreciable amounts of lipid from the serum used in the medium. For the MB III strain of mouse lymphoblasts, triglycerides were most rapidly utilized followed by phospholipids and thea cholesterol. This preferential utilization of triglycerides was also shown by 4 other cell strains. 2) The MB III cell utilized saturated and unsaturated lipids equally readily, and there was little net breakdown of lipid to satisfy cell energy requirements. There was however considerable conversion of triglycerides to phospholipid following absorption. 3) Cholesterol uptake appears to be a general phenomenon, and appreciable amounts ranging from 0.6% to 2.8% of the dry weight were found in all strains of cultured cells examined. Cholesterol content of cells was related to the type of serum used in the growth medium. 4) The composition of the lipid taken up by the cells differed markedly from that in the medium. This is discussed from the point of view...
32 citations
TL;DR: Bean callus was induced to form roots (tissue differentiation) and vascular nodules (cell differentiation) by lowering the ratio of auxin to cytokinin in the growth medium and phenylalanine ammonia lyase activity of the calluses was closely correlated with the amount of cell differentiation which had occurred.
Abstract: Bean callus was induced to form roots (tissue differentiation) and vascular nodules (cell differentiation) by lowering the ratio of auxin to cytokinin in the growth medium. Both types of differentiation were inhibited by the addition of abscisic acid (at concentrations greater than I muM) to induction medium. Initiation of differentiation was inhibited, but its subsequent development was not, and the inhibition was not affected by the addition of gibberellic acid. Addition of gibberellic acid (GA) alone to induction medium stimulated tissue differentiation, although cell differentiation was unaffected (30 muM GA) or inhibited (45 muM GA) and its onset was delayed at both concentrations. Root initiation was also stimulated by gibberellic acid (0.I-45 muM) at an auxin-to-kinin ratio 10 times that normally optimal for cell differentiation. The phenylalanine ammonia lyase (PAL) activity of the calluses was closely correlated with the amount of cell differentiation which had occurred, and measurement of this confirmed that gibberellic acid delayed the initiation of cell differentiation. The increase and subsequent decline of PAL and betaI leads to 3 glucan synthetase activities, normally induced by transfer to induction medium, was abolished by abscisic acid. Addition of gibberellic acid did not affect the betaI leads to 3 glucan synthetase activity.
32 citations
TL;DR: The agar overlay method proved to be the more sensitive in detecting low concentrations of the cytotoxic agent in the plastics, while the histochemical method demonstrated cytot toxic changes in the cells much earlier than did the loss of neutral red.
32 citations
TL;DR: Three fungal strains; namely, Aspergillus terreus, Penicillium janthinellum and Penicills duclauxii were cultured on different growth media including yeast extract, malt extract, yeast-malt extract, Czapek's Dox, Sabourod's, Harrlod's, and potato dextrose.
Abstract: Three fungal strains; namely, Aspergillus terreus, Penicillium janthinellum and Penicillium duclauxii were cultured on different growth media including yeast extract, malt extract, yeast-malt extract, Czapek's Dox, Sabourod's, Harrlod's, and potato dextrose. The growth and secondary metabolites of the three fungal strains were greatly affected by the growth medium. The colour of the culture and secondary metabolites were noticeably altered and changed according to the growth medium used.
32 citations
TL;DR: An anaerobic gram-positive coccus was isolated from the cecal contents of adult chickens and Cultures of the bacteria inhibited the growth of Salmonella typhimurium and Escherichia coli 0157:H7 on media containing either 0.25 or 2.5% lactose.
32 citations