Topic
Growth medium
About: Growth medium is a research topic. Over the lifetime, 1889 publications have been published within this topic receiving 59171 citations. The topic is also known as: culture medium & culture media.
Papers published on a yearly basis
Papers
More filters
TL;DR: The presence of 2,3-dihydroxy benzoic acid (DHBA) and 3,4-DHBA in the siderophore extract induced growth ofRhizobium in a medium containing EDTA and addition of lysine and alanine in the growth medium led to a fourfold increase in siderophile production.
Abstract: In an iron-depleted broth culture of cowpeaRhizobium (a peanut isolate), phenolate type of compounds were detected. Chemical characterization showed the presence of 2,3-dihydroxy benzoic acid (DHBA) and 3,4-DHBA in the siderophore extract. Lysine and alanine were identified as conjugated amino acids of the siderophore. Maximum concentration of the siderophore in the culture supernatant was found after 24 h of growth. The compounds in the extracted siderophore induced growth ofRhizobium in a medium containing EDTA. Addition of lysine and alanine in the growth medium (20 mM each) led to a fourfold increase in siderophore production.
31 citations
TL;DR: The phenomenon of drug adaptation in Tetrahymena, at least with respect to cycloheximide, is associated with this ribosome phosphorylation, and enzymatic removal of this phosphate converts "starved cell" ribosomes into "growing cell" Ribosomes.
31 citations
TL;DR: Maximal phosphatidylinositol:ceramide phosphoinositol transferase activity was measured in yeast cells harvested during the exponential phase of growth and enzyme activity was not regulated in yeast inositol biosynthesis regulatory mutants by the addition of inositols to the growth medium.
Abstract: Maximal phosphatidylinositol:ceramide phosphoinositol transferase activity was measured in yeast cells harvested during the exponential phase of growth. The addition of inositol to the growth medium resulted in a twofold increase in IPC synthase activity in cells grown in the presence or absence of exogenous choline. Enzyme activity was not regulated in yeast inositol biosynthesis regulatory mutants by the addition of inositol to the growth medium.
31 citations
TL;DR: Evidence is presented to support the idea that the growth processes for Euglena utilizing acetate, malate, and possibly fumarate, are more sensitive to pH than are the respiration processes, and between differential permeability of ions and free acids are discussed.
31 citations
TL;DR: Mn(2+) may play a role in the inducement of the heat-shock requirement and the formation of spores with increased heat resistance in Bacillus fastidiosus, which requires uric acid or allantoin on a simple medium.
Abstract: Bacillus fastidiosus, which requires uric acid or allantoin, grows and sporulates on a simple medium containing 59.5 mM uric acid, 5.7 mM K(2)HPO(4), and 2% agar in distilled water. Seventy to ninety percent sporulation was achieved in 96 h. Spores obtained on this medium do not need a heat shock prior to germination. The necessary germination conditions for this organism are 30 C, phosphate or this(hydroxymethyl)aminomethane buffer at pH 7.0, and 5.95 mM uric acid. Sporulation occurred earlier (48 h) and with higher frequency (greater than 99%) when Mn(2+) was added to the growth medium. However, these spores germinated only after heat activation (70 C, 30 min). The effectiveness of heat activation was directly dependent upon the concentration of Mn(2+) in the growth medium; 10(-5) M Mn(2+) was the minimal concentration for the effect. This phenomenon was not found upon addition of Ca(2+), Mg(2+), Fe(2+), Zn(2+), or Cu(2+) to the medium. The Mn(2+) content of the spores depended upon the concentration of Mn(2+) in the sporulation medium. There was a significant difference in heat resistance between spores harvested from unsupplemented medium and those harvested from medium supplemented with 5 x 10(-5) M Mn(2+). A D(85 C) value of 6.5 min was determined with the former, whereas the latter had a value of 17.0 min. Very little change in either Ca(2+) or dipicolinic acid content was detected in spores harvested from various Mn(2+)-supplemented media. Thus Mn(2+) may play a role in the inducement of the heat-shock requirement and the formation of spores with increased heat resistance.
31 citations