Growth medium

About: Growth medium is a research topic. Over the lifetime, 1889 publications have been published within this topic receiving 59171 citations. The topic is also known as: culture medium & culture media.

Enhanced production of napthoquinone metabolite (shikonin) from cell suspension culture of Arnebia sp. and its up-scaling through bioreactor

Abstract: Cell culture in shake flask and air-lift bioreactor was carried out to exploit the potential of Arnebia sp. for napthoquinone metabolite production. Cell suspension cultures of Arnebia were established from friable callus in liquid MS medium supplemented with 6-benzylaminopurine (BAP) (10 μM) and indole-3-butyric acid (IBA) (5 μM). Growth kinetic studies were done by using settled cell volume and fresh/dry cell weight method. Suspension cultures were maintained by sub-culturing at 10 days interval. A two-stage culture system is employed using growth medium (GM) and modified M9 medium (production medium) for cell biomass and naphthoquinone pigment production, respectively. Results showed that cultivation of cells under dark conditions at room temperature (25 ± 2 °C) enhanced the cell biomass from 100 to 625 g l−1. The pigment production was also found to be increased in dark conditions at room temperature. Alkaline pH found to have positive effect on pigment yield. In case of M9 medium constituents, absence of Na2SO4 does not affect the pigment yield. The current approaches have the cumulative effect to meet an increased level of (25.5 μg/ml) metabolite production in air-lift bioreactor.

Growth media and saprophytic use for pichia anomala

Abstract: A biologically pure culture of a yeast of the species Pichia anomala (WRL-076). The yeast is identified as NRRL Y-30842 and is applied to a site containing a deleterious microorganism. Further disclosed is a growth medium for increasing the viablility of yeast organisms.

Requirement of nickel as an essential micronutrient for the utilization of urea in the marine cyanobacterium Synechococcus sp. PCC 7002

Abstract: Synechococcus sp. PCC 7002 cells appeared to be nitrogen limited when grown on urea without nickel in the growth medium; the amounts of chlorophyll a and phycobiliproteins decreased and glycogen accumulated to high levels. When nickel was added to the urea medium, the diagnostics of nitrogen limitation was not observed. The activity of urease was dependent on the presence of nickel no matter what nitrogen source was used for cell growth. The ureC gene encoding the α-subunit of urease was constitutively transcribed in cells grown on all nitrogen sources tested. The addition of nickel to the growth medium rapidly led to a 15-fold increase in urease activity in 2 hours. These results indicate that cells of Synechococcus sp. PCC 7002 require a nickel supplement (5 μM NiSO4 in the growth medium) to utilize urea efficiently, and that Synechococcus sp. PCC 7002 cells are nickel-starved under the laboratory conditions regularly used for this strain. In two fresh water strains of cyanobacteria, Synechocystis sp. PCC 6803 and Anabaena sp. PCC 7120, high levels of urease activity were detected without addition of nickel to the growth medium, suggesting that these fresh water strains have a high-affinity uptake system for nickel or that their nickel requirements are lower than Synechococcus sp. PCC 7002.

A defined, glucose-limited mineral medium for the cultivation of Listeria spp.

Abstract: Members of the genus Listeria are fastidious bacteria with respect to their nutritional requirements, and several minimal media described in the literature fail to support growth of all Listeria spp. Furthermore, strict limitation by a single nutrient, e.g., the carbon source, has not been demonstrated for any of the published minimal media. This is an important prerequisite for defined studies of growth and physiology, including "omics." Based on a theoretical analysis of previously published mineral media for Listeria, an improved, well-balanced growth medium was designed. It supports the growth, not only of all tested Listeria monocytogenes strains, but of all other Listeria species, with the exception of L. ivanovii. The growth performance of L. monocytogenes strain Scott A was tested in the newly designed medium; glucose served as the only carbon and energy source for growth, whereas neither the supplied amino acids nor the buffering and complexing components (MOPS [morpholinepropanesulfonic acid] and EDTA) supported growth. Omission of amino acids, trace elements, or vitamins, alone or in combination, resulted in considerably reduced biomass yields. Furthermore, we monitored the specific growth rates of various Listeria strains cultivated in the designed mineral medium and compared them to growth in complex medium (brain heart infusion broth [BHI]). The novel mineral medium was optimized for the commonly used strain L. monocytogenes Scott A to achieve optimum cell yields and maximum specific growth rates. This mineral medium is the first published synthetic medium for Listeria that has been shown to be strictly carbon (glucose) limited.